Influence of duodenal infusion of L-methionine and L-methionine-dl-sulfoxide on amino acid concentration and duodenal electrical activity in Friesian calves

R Puchala, R Zabielski, V Lesniewska, P Kiela, W Barej

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3 Citations (Scopus)

Abstract

Four, 28-day-old Friesian calves (50 +/- 3 kg) fitted with duodenal, jugular catheters and duodenal electrodes were used to study absorption of duodenally infused L-methionine (Met) and L-methionine sulfoxide (MSO) and their effect on duodenal motor migrating complexes (MMC). Animals were fed milk replacer at 5% BW twice daily, but were starved overnight prior to experimental procedure. Animals received saline infusion for 2 h at 1 ml min(-1) prior to a 1-h infusion of 3.6 mmol of either Met or MSO. Infusion of saline was continued for another 2 h after cessation of amino acid infusion. Duodenal MMC were measured with the computer-based data acquisition system (MacLab, ADI, Australia). Initial plasma concentrations of Met were similar for both Met or MSO (19 mu M) infusion. Plasma Met concentrations increased 20 min after initiating Met infusion. The highest plasma Met concentration (62 mu M) occurred 15 min after Met infusion was ceased. Plasma MSO concentrations increased 40 min after the start of MSO infusion. The highest plasma MSO concentration occurred 45 min after cessation of MSO infusion (25 mu M) No change in plasma Met concentration (P > 0.1) occurred with MSO infusion. Absorption of MSO was 52% that of Met. Met did not change the duration of MMC but reduced the number of spikes during the phase of irregular spiking activity (305.5 vs 660.3 spikes min(-1) MSO; P < 0.05), MSO prolonged the duration of irregular spiking activity (30.2 vs 22.3 min Met; P < 0.05). The velocity of migration of regular spiking activity increased as a result of Met infusion (39.3 vs 21.2 cm min(-1) MSO; P < 0.001). Lack of change in Met concentration during MSO infusion indicates very little, if any, reduction of MSO in calves. Met at high concentrations may limit the absorption of nutrients through increased passage rate and decreased electrical activity during the absorption phase of MMC. (C) 1997 Elsevier Science B.V.
LanguageEnglish
Pages75-84
JournalAnimal Feed Science and Technology
Volume66
Issue number1-4
Publication statusPublished - May 1997

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Methionine
Amino Acids
Migrating Myoelectric Complexes
methionine sulfoxide
Information Systems
Electrodes
Milk
Neck
Catheters

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@article{42a5715aa66747238a1212575cf2e6d4,
title = "Influence of duodenal infusion of L-methionine and L-methionine-dl-sulfoxide on amino acid concentration and duodenal electrical activity in Friesian calves",
abstract = "Four, 28-day-old Friesian calves (50 +/- 3 kg) fitted with duodenal, jugular catheters and duodenal electrodes were used to study absorption of duodenally infused L-methionine (Met) and L-methionine sulfoxide (MSO) and their effect on duodenal motor migrating complexes (MMC). Animals were fed milk replacer at 5{\%} BW twice daily, but were starved overnight prior to experimental procedure. Animals received saline infusion for 2 h at 1 ml min(-1) prior to a 1-h infusion of 3.6 mmol of either Met or MSO. Infusion of saline was continued for another 2 h after cessation of amino acid infusion. Duodenal MMC were measured with the computer-based data acquisition system (MacLab, ADI, Australia). Initial plasma concentrations of Met were similar for both Met or MSO (19 mu M) infusion. Plasma Met concentrations increased 20 min after initiating Met infusion. The highest plasma Met concentration (62 mu M) occurred 15 min after Met infusion was ceased. Plasma MSO concentrations increased 40 min after the start of MSO infusion. The highest plasma MSO concentration occurred 45 min after cessation of MSO infusion (25 mu M) No change in plasma Met concentration (P > 0.1) occurred with MSO infusion. Absorption of MSO was 52{\%} that of Met. Met did not change the duration of MMC but reduced the number of spikes during the phase of irregular spiking activity (305.5 vs 660.3 spikes min(-1) MSO; P < 0.05), MSO prolonged the duration of irregular spiking activity (30.2 vs 22.3 min Met; P < 0.05). The velocity of migration of regular spiking activity increased as a result of Met infusion (39.3 vs 21.2 cm min(-1) MSO; P < 0.001). Lack of change in Met concentration during MSO infusion indicates very little, if any, reduction of MSO in calves. Met at high concentrations may limit the absorption of nutrients through increased passage rate and decreased electrical activity during the absorption phase of MMC. (C) 1997 Elsevier Science B.V.",
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Influence of duodenal infusion of L-methionine and L-methionine-dl-sulfoxide on amino acid concentration and duodenal electrical activity in Friesian calves. / Puchala, R; Zabielski, R; Lesniewska, V; Kiela, P; Barej, W.

In: Animal Feed Science and Technology, Vol. 66, No. 1-4, 05.1997, p. 75-84.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of duodenal infusion of L-methionine and L-methionine-dl-sulfoxide on amino acid concentration and duodenal electrical activity in Friesian calves

AU - Puchala, R

AU - Zabielski, R

AU - Lesniewska, V

AU - Kiela, P

AU - Barej, W

PY - 1997/5

Y1 - 1997/5

N2 - Four, 28-day-old Friesian calves (50 +/- 3 kg) fitted with duodenal, jugular catheters and duodenal electrodes were used to study absorption of duodenally infused L-methionine (Met) and L-methionine sulfoxide (MSO) and their effect on duodenal motor migrating complexes (MMC). Animals were fed milk replacer at 5% BW twice daily, but were starved overnight prior to experimental procedure. Animals received saline infusion for 2 h at 1 ml min(-1) prior to a 1-h infusion of 3.6 mmol of either Met or MSO. Infusion of saline was continued for another 2 h after cessation of amino acid infusion. Duodenal MMC were measured with the computer-based data acquisition system (MacLab, ADI, Australia). Initial plasma concentrations of Met were similar for both Met or MSO (19 mu M) infusion. Plasma Met concentrations increased 20 min after initiating Met infusion. The highest plasma Met concentration (62 mu M) occurred 15 min after Met infusion was ceased. Plasma MSO concentrations increased 40 min after the start of MSO infusion. The highest plasma MSO concentration occurred 45 min after cessation of MSO infusion (25 mu M) No change in plasma Met concentration (P > 0.1) occurred with MSO infusion. Absorption of MSO was 52% that of Met. Met did not change the duration of MMC but reduced the number of spikes during the phase of irregular spiking activity (305.5 vs 660.3 spikes min(-1) MSO; P < 0.05), MSO prolonged the duration of irregular spiking activity (30.2 vs 22.3 min Met; P < 0.05). The velocity of migration of regular spiking activity increased as a result of Met infusion (39.3 vs 21.2 cm min(-1) MSO; P < 0.001). Lack of change in Met concentration during MSO infusion indicates very little, if any, reduction of MSO in calves. Met at high concentrations may limit the absorption of nutrients through increased passage rate and decreased electrical activity during the absorption phase of MMC. (C) 1997 Elsevier Science B.V.

AB - Four, 28-day-old Friesian calves (50 +/- 3 kg) fitted with duodenal, jugular catheters and duodenal electrodes were used to study absorption of duodenally infused L-methionine (Met) and L-methionine sulfoxide (MSO) and their effect on duodenal motor migrating complexes (MMC). Animals were fed milk replacer at 5% BW twice daily, but were starved overnight prior to experimental procedure. Animals received saline infusion for 2 h at 1 ml min(-1) prior to a 1-h infusion of 3.6 mmol of either Met or MSO. Infusion of saline was continued for another 2 h after cessation of amino acid infusion. Duodenal MMC were measured with the computer-based data acquisition system (MacLab, ADI, Australia). Initial plasma concentrations of Met were similar for both Met or MSO (19 mu M) infusion. Plasma Met concentrations increased 20 min after initiating Met infusion. The highest plasma Met concentration (62 mu M) occurred 15 min after Met infusion was ceased. Plasma MSO concentrations increased 40 min after the start of MSO infusion. The highest plasma MSO concentration occurred 45 min after cessation of MSO infusion (25 mu M) No change in plasma Met concentration (P > 0.1) occurred with MSO infusion. Absorption of MSO was 52% that of Met. Met did not change the duration of MMC but reduced the number of spikes during the phase of irregular spiking activity (305.5 vs 660.3 spikes min(-1) MSO; P < 0.05), MSO prolonged the duration of irregular spiking activity (30.2 vs 22.3 min Met; P < 0.05). The velocity of migration of regular spiking activity increased as a result of Met infusion (39.3 vs 21.2 cm min(-1) MSO; P < 0.001). Lack of change in Met concentration during MSO infusion indicates very little, if any, reduction of MSO in calves. Met at high concentrations may limit the absorption of nutrients through increased passage rate and decreased electrical activity during the absorption phase of MMC. (C) 1997 Elsevier Science B.V.

M3 - Article

VL - 66

SP - 75

EP - 84

JO - Animal Feed Science and Technology

T2 - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

SN - 0377-8401

IS - 1-4

ER -