Influence of duodenal infusion of betaine or choline on blood metabolites and duodenal electrical activity in Friesian calves

R Puchala, R Zabielski, Violetta Naughton (nee Lesniewska), M Gralak, P Kiela, W Barej

Research output: Contribution to journalArticle

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Abstract

Four, 4-week old Friesian calves (BW = 50 +/- 3 kg), fitted with duodenal, portal and jugular catheters and duodenal electrodes, were used to study the metabolism of duodenally infused betaine (Bet) or choline (Chol) and their effects on blood sulphur amino acids and duodenal myoelectrical migrating complexes (MMC). Animals were fed milk replacer at 5 % BW twice daily, but were starved overnight prior to the experimental procedure. Animals received a saline infusion for 2 h at l ml/min followed by a 1 h infusion of 1.2 or 3.6 mmol of either Bet or Chol. Infusion of saline was continued for another 2 h after the cessation of the amino acid infusion. Duodenal MMC were measured with a computer-based data acquisition system (MacLab, ADI, Australia). There were no differences in measured blood metabolites between the jugular and portal vein; therefore, only average values were presented. Plasma Met concentrations increased from 20 mu M, 20 min after initiating Bet infusion, whereas a lower dose of Chol decreased plasma Met and a higher one had no effect. The highest plasma methionine (Met) concentration (29 mu M) occurred 45 min after the onset of the Bet infusion (1.2 mmol). Compared to the 3.6 mmol Bet infusion, the intraduodenal infusion of 1 2 mmol of Bet resulted in a greater area (P <0.001) under the plasma Met concentration curve (281.6 v. 73.3 mmol). A similar pattern was observed for plasma cystine concentrations. Infusion of Bet or Chol did not change the duration of MMC but Bet increased the number of spikes during the phase of low spiking activity (37.5 v. 14.6 pre-infusion, spikes/min; P <0.01). Chol had the same effect but only after the infusion ceased (29.3 v. 11.5 spikes/min; P <0.01). The velocity of migration of regular spiking activity (RSA; related to digesta transport) increased as a result of infusion (16.4 pre-infusion v. 31.3 Bet, 25.2 Chol cm/min; P <0.01). Chol caused an immediate increase in the velocity of migration of RSA, whereas with the Bet infusion an increase was observed after cessation of infusion. Increased concentrations of sulphur amino acids during Bet infusion could indicate that labile methyl groups may be limited in calves. Postruminal Bet and Chol supplementation may cause a decrease in nutrient absorption in the small intestine by increasing digesta transport.
LanguageEnglish
Pages321-327
JournalJournal of Agricultural Science
Volume131
Issue numberPart 3
Publication statusPublished - Nov 1998

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betaine
choline
calves
metabolites
blood
methionine
sulfur amino acids
digesta
portal vein
milk replacer
jugular vein
cystine
catheters
electrodes
neck
small intestine
animals

Cite this

@article{3e7290212acf444a82488c1f79961c8c,
title = "Influence of duodenal infusion of betaine or choline on blood metabolites and duodenal electrical activity in Friesian calves",
abstract = "Four, 4-week old Friesian calves (BW = 50 +/- 3 kg), fitted with duodenal, portal and jugular catheters and duodenal electrodes, were used to study the metabolism of duodenally infused betaine (Bet) or choline (Chol) and their effects on blood sulphur amino acids and duodenal myoelectrical migrating complexes (MMC). Animals were fed milk replacer at 5 {\%} BW twice daily, but were starved overnight prior to the experimental procedure. Animals received a saline infusion for 2 h at l ml/min followed by a 1 h infusion of 1.2 or 3.6 mmol of either Bet or Chol. Infusion of saline was continued for another 2 h after the cessation of the amino acid infusion. Duodenal MMC were measured with a computer-based data acquisition system (MacLab, ADI, Australia). There were no differences in measured blood metabolites between the jugular and portal vein; therefore, only average values were presented. Plasma Met concentrations increased from 20 mu M, 20 min after initiating Bet infusion, whereas a lower dose of Chol decreased plasma Met and a higher one had no effect. The highest plasma methionine (Met) concentration (29 mu M) occurred 45 min after the onset of the Bet infusion (1.2 mmol). Compared to the 3.6 mmol Bet infusion, the intraduodenal infusion of 1 2 mmol of Bet resulted in a greater area (P <0.001) under the plasma Met concentration curve (281.6 v. 73.3 mmol). A similar pattern was observed for plasma cystine concentrations. Infusion of Bet or Chol did not change the duration of MMC but Bet increased the number of spikes during the phase of low spiking activity (37.5 v. 14.6 pre-infusion, spikes/min; P <0.01). Chol had the same effect but only after the infusion ceased (29.3 v. 11.5 spikes/min; P <0.01). The velocity of migration of regular spiking activity (RSA; related to digesta transport) increased as a result of infusion (16.4 pre-infusion v. 31.3 Bet, 25.2 Chol cm/min; P <0.01). Chol caused an immediate increase in the velocity of migration of RSA, whereas with the Bet infusion an increase was observed after cessation of infusion. Increased concentrations of sulphur amino acids during Bet infusion could indicate that labile methyl groups may be limited in calves. Postruminal Bet and Chol supplementation may cause a decrease in nutrient absorption in the small intestine by increasing digesta transport.",
author = "R Puchala and R Zabielski and {Naughton (nee Lesniewska)}, Violetta and M Gralak and P Kiela and W Barej",
year = "1998",
month = "11",
language = "English",
volume = "131",
pages = "321--327",
journal = "Journal of Agricultural Science",
issn = "0021-8596",
publisher = "Cambridge University Press",
number = "Part 3",

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Influence of duodenal infusion of betaine or choline on blood metabolites and duodenal electrical activity in Friesian calves. / Puchala, R; Zabielski, R; Naughton (nee Lesniewska), Violetta; Gralak, M; Kiela, P; Barej, W.

In: Journal of Agricultural Science, Vol. 131, No. Part 3, 11.1998, p. 321-327.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of duodenal infusion of betaine or choline on blood metabolites and duodenal electrical activity in Friesian calves

AU - Puchala, R

AU - Zabielski, R

AU - Naughton (nee Lesniewska), Violetta

AU - Gralak, M

AU - Kiela, P

AU - Barej, W

PY - 1998/11

Y1 - 1998/11

N2 - Four, 4-week old Friesian calves (BW = 50 +/- 3 kg), fitted with duodenal, portal and jugular catheters and duodenal electrodes, were used to study the metabolism of duodenally infused betaine (Bet) or choline (Chol) and their effects on blood sulphur amino acids and duodenal myoelectrical migrating complexes (MMC). Animals were fed milk replacer at 5 % BW twice daily, but were starved overnight prior to the experimental procedure. Animals received a saline infusion for 2 h at l ml/min followed by a 1 h infusion of 1.2 or 3.6 mmol of either Bet or Chol. Infusion of saline was continued for another 2 h after the cessation of the amino acid infusion. Duodenal MMC were measured with a computer-based data acquisition system (MacLab, ADI, Australia). There were no differences in measured blood metabolites between the jugular and portal vein; therefore, only average values were presented. Plasma Met concentrations increased from 20 mu M, 20 min after initiating Bet infusion, whereas a lower dose of Chol decreased plasma Met and a higher one had no effect. The highest plasma methionine (Met) concentration (29 mu M) occurred 45 min after the onset of the Bet infusion (1.2 mmol). Compared to the 3.6 mmol Bet infusion, the intraduodenal infusion of 1 2 mmol of Bet resulted in a greater area (P <0.001) under the plasma Met concentration curve (281.6 v. 73.3 mmol). A similar pattern was observed for plasma cystine concentrations. Infusion of Bet or Chol did not change the duration of MMC but Bet increased the number of spikes during the phase of low spiking activity (37.5 v. 14.6 pre-infusion, spikes/min; P <0.01). Chol had the same effect but only after the infusion ceased (29.3 v. 11.5 spikes/min; P <0.01). The velocity of migration of regular spiking activity (RSA; related to digesta transport) increased as a result of infusion (16.4 pre-infusion v. 31.3 Bet, 25.2 Chol cm/min; P <0.01). Chol caused an immediate increase in the velocity of migration of RSA, whereas with the Bet infusion an increase was observed after cessation of infusion. Increased concentrations of sulphur amino acids during Bet infusion could indicate that labile methyl groups may be limited in calves. Postruminal Bet and Chol supplementation may cause a decrease in nutrient absorption in the small intestine by increasing digesta transport.

AB - Four, 4-week old Friesian calves (BW = 50 +/- 3 kg), fitted with duodenal, portal and jugular catheters and duodenal electrodes, were used to study the metabolism of duodenally infused betaine (Bet) or choline (Chol) and their effects on blood sulphur amino acids and duodenal myoelectrical migrating complexes (MMC). Animals were fed milk replacer at 5 % BW twice daily, but were starved overnight prior to the experimental procedure. Animals received a saline infusion for 2 h at l ml/min followed by a 1 h infusion of 1.2 or 3.6 mmol of either Bet or Chol. Infusion of saline was continued for another 2 h after the cessation of the amino acid infusion. Duodenal MMC were measured with a computer-based data acquisition system (MacLab, ADI, Australia). There were no differences in measured blood metabolites between the jugular and portal vein; therefore, only average values were presented. Plasma Met concentrations increased from 20 mu M, 20 min after initiating Bet infusion, whereas a lower dose of Chol decreased plasma Met and a higher one had no effect. The highest plasma methionine (Met) concentration (29 mu M) occurred 45 min after the onset of the Bet infusion (1.2 mmol). Compared to the 3.6 mmol Bet infusion, the intraduodenal infusion of 1 2 mmol of Bet resulted in a greater area (P <0.001) under the plasma Met concentration curve (281.6 v. 73.3 mmol). A similar pattern was observed for plasma cystine concentrations. Infusion of Bet or Chol did not change the duration of MMC but Bet increased the number of spikes during the phase of low spiking activity (37.5 v. 14.6 pre-infusion, spikes/min; P <0.01). Chol had the same effect but only after the infusion ceased (29.3 v. 11.5 spikes/min; P <0.01). The velocity of migration of regular spiking activity (RSA; related to digesta transport) increased as a result of infusion (16.4 pre-infusion v. 31.3 Bet, 25.2 Chol cm/min; P <0.01). Chol caused an immediate increase in the velocity of migration of RSA, whereas with the Bet infusion an increase was observed after cessation of infusion. Increased concentrations of sulphur amino acids during Bet infusion could indicate that labile methyl groups may be limited in calves. Postruminal Bet and Chol supplementation may cause a decrease in nutrient absorption in the small intestine by increasing digesta transport.

M3 - Article

VL - 131

SP - 321

EP - 327

JO - Journal of Agricultural Science

T2 - Journal of Agricultural Science

JF - Journal of Agricultural Science

SN - 0021-8596

IS - Part 3

ER -