TY - JOUR
T1 - Inexpensive and fast pathogenic bacteria screening using field-effect transistors
AU - Formisano, Nello
AU - Bhalla, Nikhil
AU - Heeran, Mel
AU - Reyes Martinez, Juana
AU - Sarkar, Amrita
AU - Laabei, Maisem
AU - Jolly, Pawan
AU - Bowen, Chris R.
AU - Taylor, John T.
AU - Flitsch, Sabine
AU - Estrela, Pedro
PY - 2016/11/15
Y1 - 2016/11/15
N2 - While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that often involve culturing which can be time-consuming. Therefore, innovative, simple, rapid and low-cost solutions to effectively reduce the burden of bacterial infections are urgently needed. Here we demonstrate a label-free sensor for fast bacterial detection based on metal-oxide-semiconductor field-effect transistors (MOSFETs). The electric charge of bacteria binding to the glycosylated gates of a MOSFET enables quantification in a straightforward manner. We show that the limit of quantitation is 1.9×105 CFU/mL with this simple device, which is more than 10,000-times lower than is achieved with electrochemical impedance spectroscopy (EIS) and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same modified surfaces. Moreover, the measurements are extremely fast and the sensor can be mass produced at trivial cost as a tool for initial screening of pathogens.
AB - While pathogenic bacteria contribute to a large number of globally important diseases and infections, current clinical diagnosis is based on processes that often involve culturing which can be time-consuming. Therefore, innovative, simple, rapid and low-cost solutions to effectively reduce the burden of bacterial infections are urgently needed. Here we demonstrate a label-free sensor for fast bacterial detection based on metal-oxide-semiconductor field-effect transistors (MOSFETs). The electric charge of bacteria binding to the glycosylated gates of a MOSFET enables quantification in a straightforward manner. We show that the limit of quantitation is 1.9×105 CFU/mL with this simple device, which is more than 10,000-times lower than is achieved with electrochemical impedance spectroscopy (EIS) and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-ToF) on the same modified surfaces. Moreover, the measurements are extremely fast and the sensor can be mass produced at trivial cost as a tool for initial screening of pathogens.
KW - Bacteria
KW - BioFET
KW - Biosensors
KW - Electrochemical impedance spectroscopy
KW - MALDI-ToF
UR - http://www.scopus.com/inward/record.url?scp=84964807239&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2016.04.063
DO - 10.1016/j.bios.2016.04.063
M3 - Article
C2 - 27156019
AN - SCOPUS:84964807239
SN - 0956-5663
VL - 85
SP - 103
EP - 109
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -