Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies

Krishnan Anand; Naeem Sheik Abdul; Terisha Ghazi; Muthusamy Ramesh; Gaurav Gupta; Murtaza M. Tambuwala; Harish Dureja; Sachin Kumar Singh; Dinesh Kumar Chellappan; Kamal Dua; Boomi Pandi; Muthupandian Saravanan; Anil Amichund Chuturgoon

doi:10.1021/acsomega.0c04461

Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies

Krishnan Anand, Naeem Sheik Abdul, Terisha Ghazi, Muthusamy Ramesh, Gaurav Gupta, Murtaza M. Tambuwala, Harish Dureja, Sachin Kumar Singh, Dinesh Kumar Chellappan, Kamal Dua, Boomi Pandi, Muthupandian Saravanan, Anil Amichund Chuturgoon

Research output: Contribution to journal › Article › peer-review

Abstract

In this study, novel self-assembled carbazole-thiooctanoic acid nanoparticles
(CTNs) were synthesized from amino carbazole (a mutagen) and thiooctanoic acid (an
antioxidant). The nanoparticles were characterized using hyperspectral techniques. Then, the antiproliferative potential of CTNs was determined in HepG2 liver carcinoma cells. This study employed a solvent−antisolvent interaction method to synthesize a spherical CTN of size less than 50 nm. Moreover, CT was subsequently capped to gold nanoparticles (AuNPs) in the additional comparative studies. The CT derivative was synthesized from carbazole and lipoic acid by the amide bond formation reaction using a coupling agent.
Furthermore, it was characterized using infrared (IR), 1 H nuclear magnetic resonance,
dynamic light scattering (DLS), and transmission electron microscopy techniques. The CTcapped gold nanoparticles (CTAuNPs) were prepared from CT, chloroauric acid, and
NaBH4. The CTAuNPs were characterized using ultraviolet−visible, high-resolution TEM, DLS, and Fourier transform IR techniques. The cytotoxicity and apoptosis-inducing ability of both nanoparticles were determined in HepG2 cells. The results demonstrate that CTNs exhibit antiproliferative activity in the cancerous HepG2 cells. Moreover, molecular docking and molecular dynamics studies were
conducted to explore the therapeutic potential of CT against human EGFR suppressor protein to gain more insights into the binding mode of the CT, which may show a significant role in anticancer therapy.

Original language	English
Number of pages	13
Journal	ACS Omega
Early online date	21 Dec 2020
DOIs	https://doi.org/10.1021/acsomega.0c04461
Publication status	E-pub ahead of print - 21 Dec 2020

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Anand, K., Abdul, N. S., Ghazi, T., Ramesh, M., Gupta, G., Tambuwala, M. M., Dureja, H., Singh, S. K., Chellappan, D. K., Dua, K., Pandi, B., Saravanan, M., & Chuturgoon, A. A. (2020). Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies. ACS Omega. https://doi.org/10.1021/acsomega.0c04461

Anand, Krishnan ; Abdul, Naeem Sheik ; Ghazi, Terisha ; Ramesh, Muthusamy ; Gupta, Gaurav ; Tambuwala, Murtaza M. ; Dureja, Harish ; Singh, Sachin Kumar ; Chellappan, Dinesh Kumar ; Dua, Kamal ; Pandi, Boomi ; Saravanan, Muthupandian ; Chuturgoon, Anil Amichund. / Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies. In: ACS Omega. 2020.

@article{6ac2545e22024c63bdfebddf9734ea39,

title = "Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies",

abstract = "In this study, novel self-assembled carbazole-thiooctanoic acid nanoparticles(CTNs) were synthesized from amino carbazole (a mutagen) and thiooctanoic acid (anantioxidant). The nanoparticles were characterized using hyperspectral techniques. Then, the antiproliferative potential of CTNs was determined in HepG2 liver carcinoma cells. This study employed a solvent−antisolvent interaction method to synthesize a spherical CTN of size less than 50 nm. Moreover, CT was subsequently capped to gold nanoparticles (AuNPs) in the additional comparative studies. The CT derivative was synthesized from carbazole and lipoic acid by the amide bond formation reaction using a coupling agent.Furthermore, it was characterized using infrared (IR), 1 H nuclear magnetic resonance,dynamic light scattering (DLS), and transmission electron microscopy techniques. The CTcapped gold nanoparticles (CTAuNPs) were prepared from CT, chloroauric acid, andNaBH4. The CTAuNPs were characterized using ultraviolet−visible, high-resolution TEM, DLS, and Fourier transform IR techniques. The cytotoxicity and apoptosis-inducing ability of both nanoparticles were determined in HepG2 cells. The results demonstrate that CTNs exhibit antiproliferative activity in the cancerous HepG2 cells. Moreover, molecular docking and molecular dynamics studies wereconducted to explore the therapeutic potential of CT against human EGFR suppressor protein to gain more insights into the binding mode of the CT, which may show a significant role in anticancer therapy.",

author = "Krishnan Anand and Abdul, {Naeem Sheik} and Terisha Ghazi and Muthusamy Ramesh and Gaurav Gupta and Tambuwala, {Murtaza M.} and Harish Dureja and Singh, {Sachin Kumar} and Chellappan, {Dinesh Kumar} and Kamal Dua and Boomi Pandi and Muthupandian Saravanan and Chuturgoon, {Anil Amichund}",

year = "2020",

month = dec,

day = "21",

doi = "10.1021/acsomega.0c04461",

language = "English",

journal = "ACS Omega",

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Anand, K, Abdul, NS, Ghazi, T, Ramesh, M, Gupta, G, Tambuwala, MM, Dureja, H, Singh, SK, Chellappan, DK, Dua, K, Pandi, B, Saravanan, M & Chuturgoon, AA 2020, 'Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies', ACS Omega. https://doi.org/10.1021/acsomega.0c04461

Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies. / Anand, Krishnan; Abdul, Naeem Sheik; Ghazi, Terisha; Ramesh, Muthusamy; Gupta, Gaurav; Tambuwala, Murtaza M.; Dureja, Harish; Singh, Sachin Kumar; Chellappan, Dinesh Kumar; Dua, Kamal; Pandi, Boomi; Saravanan, Muthupandian; Chuturgoon, Anil Amichund.

In: ACS Omega, 21.12.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Induction of Caspase-Mediated Apoptosis in HepG2 Liver Carcinoma Cells Using Mutagen–Antioxidant Conjugated Self-Assembled Novel Carbazole Nanoparticles and In Silico Modeling Studies

AU - Anand, Krishnan

AU - Abdul, Naeem Sheik

AU - Ghazi, Terisha

AU - Ramesh, Muthusamy

AU - Gupta, Gaurav

AU - Tambuwala, Murtaza M.

AU - Dureja, Harish

AU - Singh, Sachin Kumar

AU - Chellappan, Dinesh Kumar

AU - Dua, Kamal

AU - Pandi, Boomi

AU - Saravanan, Muthupandian

AU - Chuturgoon, Anil Amichund

PY - 2020/12/21

Y1 - 2020/12/21

N2 - In this study, novel self-assembled carbazole-thiooctanoic acid nanoparticles(CTNs) were synthesized from amino carbazole (a mutagen) and thiooctanoic acid (anantioxidant). The nanoparticles were characterized using hyperspectral techniques. Then, the antiproliferative potential of CTNs was determined in HepG2 liver carcinoma cells. This study employed a solvent−antisolvent interaction method to synthesize a spherical CTN of size less than 50 nm. Moreover, CT was subsequently capped to gold nanoparticles (AuNPs) in the additional comparative studies. The CT derivative was synthesized from carbazole and lipoic acid by the amide bond formation reaction using a coupling agent.Furthermore, it was characterized using infrared (IR), 1 H nuclear magnetic resonance,dynamic light scattering (DLS), and transmission electron microscopy techniques. The CTcapped gold nanoparticles (CTAuNPs) were prepared from CT, chloroauric acid, andNaBH4. The CTAuNPs were characterized using ultraviolet−visible, high-resolution TEM, DLS, and Fourier transform IR techniques. The cytotoxicity and apoptosis-inducing ability of both nanoparticles were determined in HepG2 cells. The results demonstrate that CTNs exhibit antiproliferative activity in the cancerous HepG2 cells. Moreover, molecular docking and molecular dynamics studies wereconducted to explore the therapeutic potential of CT against human EGFR suppressor protein to gain more insights into the binding mode of the CT, which may show a significant role in anticancer therapy.

AB - In this study, novel self-assembled carbazole-thiooctanoic acid nanoparticles(CTNs) were synthesized from amino carbazole (a mutagen) and thiooctanoic acid (anantioxidant). The nanoparticles were characterized using hyperspectral techniques. Then, the antiproliferative potential of CTNs was determined in HepG2 liver carcinoma cells. This study employed a solvent−antisolvent interaction method to synthesize a spherical CTN of size less than 50 nm. Moreover, CT was subsequently capped to gold nanoparticles (AuNPs) in the additional comparative studies. The CT derivative was synthesized from carbazole and lipoic acid by the amide bond formation reaction using a coupling agent.Furthermore, it was characterized using infrared (IR), 1 H nuclear magnetic resonance,dynamic light scattering (DLS), and transmission electron microscopy techniques. The CTcapped gold nanoparticles (CTAuNPs) were prepared from CT, chloroauric acid, andNaBH4. The CTAuNPs were characterized using ultraviolet−visible, high-resolution TEM, DLS, and Fourier transform IR techniques. The cytotoxicity and apoptosis-inducing ability of both nanoparticles were determined in HepG2 cells. The results demonstrate that CTNs exhibit antiproliferative activity in the cancerous HepG2 cells. Moreover, molecular docking and molecular dynamics studies wereconducted to explore the therapeutic potential of CT against human EGFR suppressor protein to gain more insights into the binding mode of the CT, which may show a significant role in anticancer therapy.

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DO - 10.1021/acsomega.0c04461

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JF - ACS Omega

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