TY - JOUR
T1 - Independent and grouped 3D cell rotation in a microfluidic device for bioimaging applications
AU - Puttaswamy, Srinivasu
AU - Bhalla, Nikhil
AU - Kelsey, CP
AU - Lubarsky, Gennady
AU - Lee, Chengkuo
AU - McLaughlin, James
N1 - Funding Information:
This work was supported by funding under the Invest Northern Ireland - Connected Health Innovation Centre (CHIC) Competence Centre and the European Union's INTERREG VA Programme, managed by the Special EU Programmes Body (SEUPB) – the Eastern Corridor for Medical Engineering (ECME).
Publisher Copyright:
© 2020 Elsevier B.V.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/12/15
Y1 - 2020/12/15
N2 - Cell rotation reveals important information which facilitates identification and characterization of different cells. Markedly, achieving three dimensional (3D) rolling rotation of single cells within a larger group of cells is rare among existing cell rotation techniques. In this work we present a simple biochip which can be used to trap and rotate a single cell, or to rotate multiple cells relative to each other within a group of individual red blood cells (RBCs), which is crucial for imaging cells in 3D. To achieve single RBC trapping, we employ two parallel sidewall 3D electrodes to produce a dielectrophoretic force which traps cells inside the capturing chambers of the microfluidic device, where the hydrodynamic force then induces precise rotation of the cell inside the chamber. We have also demonstrated the possibility of using the developed biochip to preconcentrate and rotate RBC clusters in 3D. As our proposed cell trapping and rotation device reduces the intricacy of cell rotation, the developed technique may have important implications for high resolution 3D cell imaging in the investigation of complex cell dynamics and interactions in moving media.
AB - Cell rotation reveals important information which facilitates identification and characterization of different cells. Markedly, achieving three dimensional (3D) rolling rotation of single cells within a larger group of cells is rare among existing cell rotation techniques. In this work we present a simple biochip which can be used to trap and rotate a single cell, or to rotate multiple cells relative to each other within a group of individual red blood cells (RBCs), which is crucial for imaging cells in 3D. To achieve single RBC trapping, we employ two parallel sidewall 3D electrodes to produce a dielectrophoretic force which traps cells inside the capturing chambers of the microfluidic device, where the hydrodynamic force then induces precise rotation of the cell inside the chamber. We have also demonstrated the possibility of using the developed biochip to preconcentrate and rotate RBC clusters in 3D. As our proposed cell trapping and rotation device reduces the intricacy of cell rotation, the developed technique may have important implications for high resolution 3D cell imaging in the investigation of complex cell dynamics and interactions in moving media.
KW - 3D-rotation
KW - Cell-imaging
KW - Cell-trapping
KW - Dielectrophoresis
UR - http://www.scopus.com/inward/record.url?scp=85092052867&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2020.112661
DO - 10.1016/j.bios.2020.112661
M3 - Article
C2 - 33032194
VL - 170
SP - 1
EP - 7
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
M1 - 112661
ER -