Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension

David Gibson, Laura Pascoli, Catherine McAllister, Catriona Scaife, Michael Dunn, Stephen Pennington, Madeleine Rooney

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Background: Juvenile idiopathic arthritis (JIA) comprises a poorlyunderstood group of chronic, childhood onset, autoimmune diseaseswith variable clinical presentations, outcomes and therapeuticresponses. Current laboratory tests are unable to flag those patientsat a higher risk of disease spread to multiple joints, who could benefitform earlier therapy to prevent joint damage. This study was focusedon profiling the synovial fluid (SF) proteome associated with diseaseextension from oligo- to polyarticular status by a difference gelelectrophoresis (DIGE) approach.Methods: To construct a discriminant model, SF samples from 55 JIApatients were analysed: 30 oligo-, 8 extended oligo- and 17polyarticular disease. Initial SF samples from each patient werelabeled with Cy dyes and subjected to protein separation by 2-DE. Theability to distinguish patients at risk of disease extension by a selectgroup of proteins was illustrated by multivariate analysis methods.Proteins over expressed with a two-fold difference between patientsubgroups were identified by MALDI-TOF. Specific antibodies wereused to validate putative biomarker expression in synovial fluid bywestern immunoblotting and in synovial membrane (SM) byimmunohistochemistry.Results: Samespots software analysis of SF gel scans was used tohighlight joint-specific proteins which were differentially expressedacross disease classifications. Hierarchical clustering based on theexpression levels of a previously selected set of 40 proteins matchedacross the three clinical subgroups segregates the extended oligoarticularpatients. Proteolytic fragments of apolipoprotein AII, complementcomponent C3c and vitamin D binding protein were identified(P
LanguageEnglish
Title of host publicationUnknown Host Publication
Pagesi86-i89
Number of pages4
Volume49
DOIs
Publication statusPublished - Jun 2012
EventBritish Society for Rheumatology Annual Meeting - Birmingham, UK
Duration: 1 Jun 2012 → …

Conference

ConferenceBritish Society for Rheumatology Annual Meeting
Period1/06/12 → …

Fingerprint

Vitamin D-Binding Protein
Juvenile Arthritis
Synovial Fluid
Joints
Proteins
Apolipoprotein A-II
Synovial Membrane
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Proteome
Secondary Prevention
Immunoblotting
Cluster Analysis
Coloring Agents
Software
Multivariate Analysis
Biomarkers
Gels
Antibodies

Keywords

  • Proteomics
  • Vitamin D binding protein
  • Juvenile arthritis
  • biomarker

Cite this

Gibson, D., Pascoli, L., McAllister, C., Scaife, C., Dunn, M., Pennington, S., & Rooney, M. (2012). Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension. In Unknown Host Publication (Vol. 49, pp. i86-i89) https://doi.org/10.1093/rheumatology/keq724
Gibson, David ; Pascoli, Laura ; McAllister, Catherine ; Scaife, Catriona ; Dunn, Michael ; Pennington, Stephen ; Rooney, Madeleine. / Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension. Unknown Host Publication. Vol. 49 2012. pp. i86-i89
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Gibson, D, Pascoli, L, McAllister, C, Scaife, C, Dunn, M, Pennington, S & Rooney, M 2012, Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension. in Unknown Host Publication. vol. 49, pp. i86-i89, British Society for Rheumatology Annual Meeting, 1/06/12. https://doi.org/10.1093/rheumatology/keq724

Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension. / Gibson, David; Pascoli, Laura; McAllister, Catherine; Scaife, Catriona; Dunn, Michael; Pennington, Stephen; Rooney, Madeleine.

Unknown Host Publication. Vol. 49 2012. p. i86-i89.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension

AU - Gibson, David

AU - Pascoli, Laura

AU - McAllister, Catherine

AU - Scaife, Catriona

AU - Dunn, Michael

AU - Pennington, Stephen

AU - Rooney, Madeleine

PY - 2012/6

Y1 - 2012/6

N2 - Background: Juvenile idiopathic arthritis (JIA) comprises a poorlyunderstood group of chronic, childhood onset, autoimmune diseaseswith variable clinical presentations, outcomes and therapeuticresponses. Current laboratory tests are unable to flag those patientsat a higher risk of disease spread to multiple joints, who could benefitform earlier therapy to prevent joint damage. This study was focusedon profiling the synovial fluid (SF) proteome associated with diseaseextension from oligo- to polyarticular status by a difference gelelectrophoresis (DIGE) approach.Methods: To construct a discriminant model, SF samples from 55 JIApatients were analysed: 30 oligo-, 8 extended oligo- and 17polyarticular disease. Initial SF samples from each patient werelabeled with Cy dyes and subjected to protein separation by 2-DE. Theability to distinguish patients at risk of disease extension by a selectgroup of proteins was illustrated by multivariate analysis methods.Proteins over expressed with a two-fold difference between patientsubgroups were identified by MALDI-TOF. Specific antibodies wereused to validate putative biomarker expression in synovial fluid bywestern immunoblotting and in synovial membrane (SM) byimmunohistochemistry.Results: Samespots software analysis of SF gel scans was used tohighlight joint-specific proteins which were differentially expressedacross disease classifications. Hierarchical clustering based on theexpression levels of a previously selected set of 40 proteins matchedacross the three clinical subgroups segregates the extended oligoarticularpatients. Proteolytic fragments of apolipoprotein AII, complementcomponent C3c and vitamin D binding protein were identified(P

AB - Background: Juvenile idiopathic arthritis (JIA) comprises a poorlyunderstood group of chronic, childhood onset, autoimmune diseaseswith variable clinical presentations, outcomes and therapeuticresponses. Current laboratory tests are unable to flag those patientsat a higher risk of disease spread to multiple joints, who could benefitform earlier therapy to prevent joint damage. This study was focusedon profiling the synovial fluid (SF) proteome associated with diseaseextension from oligo- to polyarticular status by a difference gelelectrophoresis (DIGE) approach.Methods: To construct a discriminant model, SF samples from 55 JIApatients were analysed: 30 oligo-, 8 extended oligo- and 17polyarticular disease. Initial SF samples from each patient werelabeled with Cy dyes and subjected to protein separation by 2-DE. Theability to distinguish patients at risk of disease extension by a selectgroup of proteins was illustrated by multivariate analysis methods.Proteins over expressed with a two-fold difference between patientsubgroups were identified by MALDI-TOF. Specific antibodies wereused to validate putative biomarker expression in synovial fluid bywestern immunoblotting and in synovial membrane (SM) byimmunohistochemistry.Results: Samespots software analysis of SF gel scans was used tohighlight joint-specific proteins which were differentially expressedacross disease classifications. Hierarchical clustering based on theexpression levels of a previously selected set of 40 proteins matchedacross the three clinical subgroups segregates the extended oligoarticularpatients. Proteolytic fragments of apolipoprotein AII, complementcomponent C3c and vitamin D binding protein were identified(P

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KW - Vitamin D binding protein

KW - Juvenile arthritis

KW - biomarker

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DO - 10.1093/rheumatology/keq724

M3 - Conference contribution

VL - 49

SP - i86-i89

BT - Unknown Host Publication

ER -

Gibson D, Pascoli L, McAllister C, Scaife C, Dunn M, Pennington S et al. Increased Vitamin D Binding Protein Expression in JIA Patients Suffering Disease Extension. In Unknown Host Publication. Vol. 49. 2012. p. i86-i89 https://doi.org/10.1093/rheumatology/keq724