INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

J SIMPSON, D BRADY, A ROLLAN, N BARRON, L MCHALE, AP MCHALE

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.
LanguageEnglish
Pages757-760
JournalBiotechnology Letters
Volume17
Issue number7
DOIs
Publication statusPublished - Jul 1995

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Kluyveromyces marxianus
ethanol production
electric field
lactose
culture filtrates
beta-galactosidase
ethanol
yeasts

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@article{b9950d6f7ceb431bae2d932bc29342eb,
title = "INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C",
abstract = "Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35{\%} of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4{\%} [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.",
author = "J SIMPSON and D BRADY and A ROLLAN and N BARRON and L MCHALE and AP MCHALE",
year = "1995",
month = "7",
doi = "10.1007/BF00130364",
language = "English",
volume = "17",
pages = "757--760",
journal = "Biotechnology Letters",
issn = "0141-5492",
number = "7",

}

INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C. / SIMPSON, J; BRADY, D; ROLLAN, A; BARRON, N; MCHALE, L; MCHALE, AP.

In: Biotechnology Letters, Vol. 17, No. 7, 07.1995, p. 757-760.

Research output: Contribution to journalArticle

TY - JOUR

T1 - INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

AU - SIMPSON, J

AU - BRADY, D

AU - ROLLAN, A

AU - BARRON, N

AU - MCHALE, L

AU - MCHALE, AP

PY - 1995/7

Y1 - 1995/7

N2 - Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

AB - Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

U2 - 10.1007/BF00130364

DO - 10.1007/BF00130364

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JO - Biotechnology Letters

T2 - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

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