Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.
SIMPSON, J., BRADY, D., ROLLAN, A., BARRON, N., MCHALE, L., & MCHALE, AP. (1995). INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C. Biotechnology Letters, 17(7), 757-760. https://doi.org/10.1007/BF00130364