INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

J SIMPSON; D BRADY; A ROLLAN; N BARRON; L MCHALE; AP MCHALE

doi:10.1007/BF00130364

INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

J SIMPSON, D BRADY, A ROLLAN, N BARRON, L MCHALE, AP MCHALE

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

Original language	English
Pages (from-to)	757-760
Journal	Biotechnology Letters
Volume	17
Issue number	7
DOIs	https://doi.org/10.1007/BF00130364
Publication status	Published (in print/issue) - Jul 1995

Access to Document

10.1007/BF00130364

Cite this

@article{b9950d6f7ceb431bae2d932bc29342eb,

title = "INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C",

abstract = "Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.",

author = "J SIMPSON and D BRADY and A ROLLAN and N BARRON and L MCHALE and AP MCHALE",

year = "1995",

month = jul,

doi = "10.1007/BF00130364",

language = "English",

volume = "17",

pages = "757--760",

journal = "Biotechnology Letters",

issn = "1573-6776",

publisher = "Springer",

number = "7",

}

TY - JOUR

T1 - INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

AU - SIMPSON, J

AU - BRADY, D

AU - ROLLAN, A

AU - BARRON, N

AU - MCHALE, L

AU - MCHALE, AP

PY - 1995/7

Y1 - 1995/7

N2 - Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

AB - Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4% [w/v]). Following treatment with electric fields and addition of 1mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.

U2 - 10.1007/BF00130364

DO - 10.1007/BF00130364

M3 - Article

SN - 1573-6776

VL - 17

SP - 757

EP - 760

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 7

ER -

INCREASED ETHANOL-PRODUCTION DURING GROWTH OF ELECTRIC-FIELD STIMULATED KLUYVEROMYCES-MARXIANUS IMB3 DURING GROWTH ON LACTOSE-CONTAINING MEDIA AT 45-DEGREES-C

Abstract

Access to Document

Fingerprint

Cite this