Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes

Antonino Fiorentino, Giovanna Ferro, María Castro Alferez, Maria Inmaculada Polo-López, Pilar Fernandez-Ibanez, Luigi Rizzo

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Solar disinfection and solar-driven advanced oxidation processes (AOPs) (namely H2O2/sunlight,TiO2/sunlight, H2O2/TiO2/sunlight, solar photo-Fenton) were evaluated in the inactivation of indigenousantibiotic-resistant bacteria (ARB) in real urban wastewater. A multidrug resistant (MDR) Escherichia colistrain isolated from the effluent of the biological process of an urban wastewater treatment plant was thetarget ARB. The higher inactivation rates (residual density under detection limit, 2 CFU mL�1) wereachieved with H2O2/TiO2/sunlight (cumulative energy per unit of volume (QUV) in the range 3–5 kJ L�1,depending on H2O2/TiO2 ratio) and H2O2/sunlight (QUV of 8 kJ L�1) processes. All investigated processesdid not affect antibiotic resistance of survived colonies. Moreover, H2O2/sunlight was compared with conventional chlorination process to evaluate bacterial regrowth potential and particularly the proportion ofindigenous MDR E. coli with respect to total indigenous E. coli population. Chlorination (1.0 mg Cl2 L�1)was more effective than H2O2/sunlight (50 mg H2O2 L�1) to achieve total inactivation of MDR E. coli(15 min Vs 90 min) but less effective in controlling their regrowth (24 h Vs 48 h). Interestingly, the percentageof MDR E. coli in H2O2/sunlight treated samples decreased as incubation time increased; theopposite was observed for chlorinated samples.
LanguageEnglish
Pages43-50
JournalJournal of Photochemistry and Photobiology B: Biology
Volume148
DOIs
Publication statusPublished - 4 Apr 2015

Fingerprint

chlorination
Sunlight
Halogenation
Disinfection
sunlight
Waste Water
deactivation
bacteria
Bacteria
Escherichia coli
Biological Phenomena
Escherichia
antibiotics
effluents
Microbial Drug Resistance
Limit of Detection
proportion
oxidation

Keywords

  • E. coli
  • Advanced Oxidation Processes
  • photocatalysis
  • photo-Fenton

Cite this

Fiorentino, Antonino ; Ferro, Giovanna ; Alferez, María Castro ; Polo-López, Maria Inmaculada ; Fernandez-Ibanez, Pilar ; Rizzo, Luigi. / Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes. 2015 ; Vol. 148. pp. 43-50.
@article{4ca0f12b2457454b94b0cb21668af07e,
title = "Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes",
abstract = "Solar disinfection and solar-driven advanced oxidation processes (AOPs) (namely H2O2/sunlight,TiO2/sunlight, H2O2/TiO2/sunlight, solar photo-Fenton) were evaluated in the inactivation of indigenousantibiotic-resistant bacteria (ARB) in real urban wastewater. A multidrug resistant (MDR) Escherichia colistrain isolated from the effluent of the biological process of an urban wastewater treatment plant was thetarget ARB. The higher inactivation rates (residual density under detection limit, 2 CFU mL�1) wereachieved with H2O2/TiO2/sunlight (cumulative energy per unit of volume (QUV) in the range 3–5 kJ L�1,depending on H2O2/TiO2 ratio) and H2O2/sunlight (QUV of 8 kJ L�1) processes. All investigated processesdid not affect antibiotic resistance of survived colonies. Moreover, H2O2/sunlight was compared with conventional chlorination process to evaluate bacterial regrowth potential and particularly the proportion ofindigenous MDR E. coli with respect to total indigenous E. coli population. Chlorination (1.0 mg Cl2 L�1)was more effective than H2O2/sunlight (50 mg H2O2 L�1) to achieve total inactivation of MDR E. coli(15 min Vs 90 min) but less effective in controlling their regrowth (24 h Vs 48 h). Interestingly, the percentageof MDR E. coli in H2O2/sunlight treated samples decreased as incubation time increased; theopposite was observed for chlorinated samples.",
keywords = "E. coli, Advanced Oxidation Processes, photocatalysis, photo-Fenton",
author = "Antonino Fiorentino and Giovanna Ferro and Alferez, {Mar{\'i}a Castro} and Polo-L{\'o}pez, {Maria Inmaculada} and Pilar Fernandez-Ibanez and Luigi Rizzo",
year = "2015",
month = "4",
day = "4",
doi = "10.1016/j.jphotobiol.2015.03.029",
language = "English",
volume = "148",
pages = "43--50",

}

Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes. / Fiorentino, Antonino; Ferro, Giovanna; Alferez, María Castro; Polo-López, Maria Inmaculada; Fernandez-Ibanez, Pilar; Rizzo, Luigi.

Vol. 148, 04.04.2015, p. 43-50.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Inactivation and regrowth of multidrug resistant bacteria in urban wastewater after disinfection by solar-driven and chlorination processes

AU - Fiorentino, Antonino

AU - Ferro, Giovanna

AU - Alferez, María Castro

AU - Polo-López, Maria Inmaculada

AU - Fernandez-Ibanez, Pilar

AU - Rizzo, Luigi

PY - 2015/4/4

Y1 - 2015/4/4

N2 - Solar disinfection and solar-driven advanced oxidation processes (AOPs) (namely H2O2/sunlight,TiO2/sunlight, H2O2/TiO2/sunlight, solar photo-Fenton) were evaluated in the inactivation of indigenousantibiotic-resistant bacteria (ARB) in real urban wastewater. A multidrug resistant (MDR) Escherichia colistrain isolated from the effluent of the biological process of an urban wastewater treatment plant was thetarget ARB. The higher inactivation rates (residual density under detection limit, 2 CFU mL�1) wereachieved with H2O2/TiO2/sunlight (cumulative energy per unit of volume (QUV) in the range 3–5 kJ L�1,depending on H2O2/TiO2 ratio) and H2O2/sunlight (QUV of 8 kJ L�1) processes. All investigated processesdid not affect antibiotic resistance of survived colonies. Moreover, H2O2/sunlight was compared with conventional chlorination process to evaluate bacterial regrowth potential and particularly the proportion ofindigenous MDR E. coli with respect to total indigenous E. coli population. Chlorination (1.0 mg Cl2 L�1)was more effective than H2O2/sunlight (50 mg H2O2 L�1) to achieve total inactivation of MDR E. coli(15 min Vs 90 min) but less effective in controlling their regrowth (24 h Vs 48 h). Interestingly, the percentageof MDR E. coli in H2O2/sunlight treated samples decreased as incubation time increased; theopposite was observed for chlorinated samples.

AB - Solar disinfection and solar-driven advanced oxidation processes (AOPs) (namely H2O2/sunlight,TiO2/sunlight, H2O2/TiO2/sunlight, solar photo-Fenton) were evaluated in the inactivation of indigenousantibiotic-resistant bacteria (ARB) in real urban wastewater. A multidrug resistant (MDR) Escherichia colistrain isolated from the effluent of the biological process of an urban wastewater treatment plant was thetarget ARB. The higher inactivation rates (residual density under detection limit, 2 CFU mL�1) wereachieved with H2O2/TiO2/sunlight (cumulative energy per unit of volume (QUV) in the range 3–5 kJ L�1,depending on H2O2/TiO2 ratio) and H2O2/sunlight (QUV of 8 kJ L�1) processes. All investigated processesdid not affect antibiotic resistance of survived colonies. Moreover, H2O2/sunlight was compared with conventional chlorination process to evaluate bacterial regrowth potential and particularly the proportion ofindigenous MDR E. coli with respect to total indigenous E. coli population. Chlorination (1.0 mg Cl2 L�1)was more effective than H2O2/sunlight (50 mg H2O2 L�1) to achieve total inactivation of MDR E. coli(15 min Vs 90 min) but less effective in controlling their regrowth (24 h Vs 48 h). Interestingly, the percentageof MDR E. coli in H2O2/sunlight treated samples decreased as incubation time increased; theopposite was observed for chlorinated samples.

KW - E. coli

KW - Advanced Oxidation Processes

KW - photocatalysis

KW - photo-Fenton

U2 - 10.1016/j.jphotobiol.2015.03.029

DO - 10.1016/j.jphotobiol.2015.03.029

M3 - Article

VL - 148

SP - 43

EP - 50

ER -