In vitro modulation of pancreatic insulin secretion, extrapancreatic insulin action and peptide glycation by Curcuma longa aqueous extracts

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Abstract

Objective: Medicinal, edible, and aromatic plants have been used as folk remedies in traditional treatments worldwide. This study investigates the antidiabetic efficacy and action mode of Curcuma longa Linn. (Zingiberaceae). Methods: Effects of aqueous extracts (AEs) of C. longa on insulin secretion and action were studied using the insulin-secreting BRIN-BD11 and the adipocyte-like 3T3-L1 cell lines, respectively. In vitro models were employed to evaluate effects on starch digestion using α-amylase/amyloglucosidase and protein glycation. Results: C. longa AEs stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic beta cell line, BRIN-BD11 (P <0.001). The insulin secretory activity of plant extract was abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake, diazoxide and verapamil (P <0.001). Furthermore, the extract increased insulin secretion in depolarized cells and augmented insulin secretion triggered by 3-isobutyl-1-methylxanthine, tolbutamide, and glibenclamide. C. longa AEs lacked insulin mimetic activity but enhanced insulin-stimulated glucose transport in 3T3-L1 adipocytes by 370% (P <0.001). Similar to aminoguanidine, C. longa AEs (1-50 mg/ml) effected concentration-dependent inhibition of protein glycation (24-70% inhibition, P <0.001) in vitro. In bioassays of enzymatic starch digestion, C. longa AEs lacked inhibitory effects on α-amylase and α-glucosidase, unlike acarbose, the classical reference drug. Conclusion: This study has revealed that water soluble bioactive principles in C. longa AEs stimulate basal- and potentiate glucose evoked-insulin secretion, enhance insulin action and inhibit insulin glycation, but not starch digestion. Future work assessing the use of C. longa AEs as dietary adjunct or as a source of active antidiabetic agents may provide new opportunities for the combinatorial treatment/prevention of diabetes.
LanguageEnglish
Pages187-193
JournalJournal of Experimental and Integrative Medicine
Volume4
Issue number3
DOIs
Publication statusPublished - 27 Jun 2014

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Curcuma
Insulin
Peptides
Starch
Digestion
alpha-Amylases
Hypoglycemic Agents
Adipocytes
Glucose
In Vitro Techniques
Zingiberaceae
Acarbose
3T3-L1 Cells
Glucan 1,4-alpha-Glucosidase
Diazoxide
1-Methyl-3-isobutylxanthine
Cell Line
Tolbutamide
Edible Plants
alpha-Glucosidases

Keywords

  • Curcuma longa Linn. (Zingiberaceae)
  • insulin action
  • insulin secretion
  • peptide glycation
  • starch digestion

Cite this

@article{e694098f279d42f5902e77a13d19e3f6,
title = "In vitro modulation of pancreatic insulin secretion, extrapancreatic insulin action and peptide glycation by Curcuma longa aqueous extracts",
abstract = "Objective: Medicinal, edible, and aromatic plants have been used as folk remedies in traditional treatments worldwide. This study investigates the antidiabetic efficacy and action mode of Curcuma longa Linn. (Zingiberaceae). Methods: Effects of aqueous extracts (AEs) of C. longa on insulin secretion and action were studied using the insulin-secreting BRIN-BD11 and the adipocyte-like 3T3-L1 cell lines, respectively. In vitro models were employed to evaluate effects on starch digestion using α-amylase/amyloglucosidase and protein glycation. Results: C. longa AEs stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic beta cell line, BRIN-BD11 (P <0.001). The insulin secretory activity of plant extract was abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake, diazoxide and verapamil (P <0.001). Furthermore, the extract increased insulin secretion in depolarized cells and augmented insulin secretion triggered by 3-isobutyl-1-methylxanthine, tolbutamide, and glibenclamide. C. longa AEs lacked insulin mimetic activity but enhanced insulin-stimulated glucose transport in 3T3-L1 adipocytes by 370{\%} (P <0.001). Similar to aminoguanidine, C. longa AEs (1-50 mg/ml) effected concentration-dependent inhibition of protein glycation (24-70{\%} inhibition, P <0.001) in vitro. In bioassays of enzymatic starch digestion, C. longa AEs lacked inhibitory effects on α-amylase and α-glucosidase, unlike acarbose, the classical reference drug. Conclusion: This study has revealed that water soluble bioactive principles in C. longa AEs stimulate basal- and potentiate glucose evoked-insulin secretion, enhance insulin action and inhibit insulin glycation, but not starch digestion. Future work assessing the use of C. longa AEs as dietary adjunct or as a source of active antidiabetic agents may provide new opportunities for the combinatorial treatment/prevention of diabetes.",
keywords = "Curcuma longa Linn. (Zingiberaceae), insulin action, insulin secretion, peptide glycation, starch digestion",
author = "V Kasabri and Peter Flatt and YHA Abdel-Wahab",
year = "2014",
month = "6",
day = "27",
doi = "10.5455/jeim.170414",
language = "English",
volume = "4",
pages = "187--193",
journal = "Journal of Experimental and Integrative Medicine",
issn = "1309-4572",
number = "3",

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TY - JOUR

T1 - In vitro modulation of pancreatic insulin secretion, extrapancreatic insulin action and peptide glycation by Curcuma longa aqueous extracts

AU - Kasabri, V

AU - Flatt, Peter

AU - Abdel-Wahab, YHA

PY - 2014/6/27

Y1 - 2014/6/27

N2 - Objective: Medicinal, edible, and aromatic plants have been used as folk remedies in traditional treatments worldwide. This study investigates the antidiabetic efficacy and action mode of Curcuma longa Linn. (Zingiberaceae). Methods: Effects of aqueous extracts (AEs) of C. longa on insulin secretion and action were studied using the insulin-secreting BRIN-BD11 and the adipocyte-like 3T3-L1 cell lines, respectively. In vitro models were employed to evaluate effects on starch digestion using α-amylase/amyloglucosidase and protein glycation. Results: C. longa AEs stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic beta cell line, BRIN-BD11 (P <0.001). The insulin secretory activity of plant extract was abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake, diazoxide and verapamil (P <0.001). Furthermore, the extract increased insulin secretion in depolarized cells and augmented insulin secretion triggered by 3-isobutyl-1-methylxanthine, tolbutamide, and glibenclamide. C. longa AEs lacked insulin mimetic activity but enhanced insulin-stimulated glucose transport in 3T3-L1 adipocytes by 370% (P <0.001). Similar to aminoguanidine, C. longa AEs (1-50 mg/ml) effected concentration-dependent inhibition of protein glycation (24-70% inhibition, P <0.001) in vitro. In bioassays of enzymatic starch digestion, C. longa AEs lacked inhibitory effects on α-amylase and α-glucosidase, unlike acarbose, the classical reference drug. Conclusion: This study has revealed that water soluble bioactive principles in C. longa AEs stimulate basal- and potentiate glucose evoked-insulin secretion, enhance insulin action and inhibit insulin glycation, but not starch digestion. Future work assessing the use of C. longa AEs as dietary adjunct or as a source of active antidiabetic agents may provide new opportunities for the combinatorial treatment/prevention of diabetes.

AB - Objective: Medicinal, edible, and aromatic plants have been used as folk remedies in traditional treatments worldwide. This study investigates the antidiabetic efficacy and action mode of Curcuma longa Linn. (Zingiberaceae). Methods: Effects of aqueous extracts (AEs) of C. longa on insulin secretion and action were studied using the insulin-secreting BRIN-BD11 and the adipocyte-like 3T3-L1 cell lines, respectively. In vitro models were employed to evaluate effects on starch digestion using α-amylase/amyloglucosidase and protein glycation. Results: C. longa AEs stimulated basal insulin output and potentiated glucose-stimulated insulin secretion concentration-dependently in the clonal pancreatic beta cell line, BRIN-BD11 (P <0.001). The insulin secretory activity of plant extract was abolished in the absence of extracellular Ca2+ and by inhibitors of cellular Ca2+ uptake, diazoxide and verapamil (P <0.001). Furthermore, the extract increased insulin secretion in depolarized cells and augmented insulin secretion triggered by 3-isobutyl-1-methylxanthine, tolbutamide, and glibenclamide. C. longa AEs lacked insulin mimetic activity but enhanced insulin-stimulated glucose transport in 3T3-L1 adipocytes by 370% (P <0.001). Similar to aminoguanidine, C. longa AEs (1-50 mg/ml) effected concentration-dependent inhibition of protein glycation (24-70% inhibition, P <0.001) in vitro. In bioassays of enzymatic starch digestion, C. longa AEs lacked inhibitory effects on α-amylase and α-glucosidase, unlike acarbose, the classical reference drug. Conclusion: This study has revealed that water soluble bioactive principles in C. longa AEs stimulate basal- and potentiate glucose evoked-insulin secretion, enhance insulin action and inhibit insulin glycation, but not starch digestion. Future work assessing the use of C. longa AEs as dietary adjunct or as a source of active antidiabetic agents may provide new opportunities for the combinatorial treatment/prevention of diabetes.

KW - Curcuma longa Linn. (Zingiberaceae)

KW - insulin action

KW - insulin secretion

KW - peptide glycation

KW - starch digestion

U2 - 10.5455/jeim.170414

DO - 10.5455/jeim.170414

M3 - Article

VL - 4

SP - 187

EP - 193

JO - Journal of Experimental and Integrative Medicine

T2 - Journal of Experimental and Integrative Medicine

JF - Journal of Experimental and Integrative Medicine

SN - 1309-4572

IS - 3

ER -