In vitro Effect of Silver Nanoparticles on Blastocystis hominis

Mohamed Saad Younis; Eman Abd El Rahman Abououf; Ali El Saeed Ali; Sara Mohamed Abd Elhady; Rita Maher Wassef

doi:10.2147/IJN.S272532

In vitro Effect of Silver Nanoparticles on Blastocystis hominis

Mohamed Saad Younis, Eman Abd El Rahman Abououf, Ali El Saeed Ali, Sara Mohamed Abd Elhady, Rita Maher Wassef

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

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Abstract

Introduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis.

Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs.

Results: Blastocystis hominis count was reduced by 20.72%, 28.23%, and 18.92% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49%, 61.61%, and 40.78% after 2 hours and by 71.69%, 79.67%, and 62.65% after 3 hours of adding the drugs in the same order, respectively.

Conclusion: There was a statistically significant difference (P<0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.

Original language	English
Pages (from-to)	8167-8173
Number of pages	7
Journal	International Journal of Nanomedicine
Volume	15
Early online date	1 Sept 2020
DOIs	https://doi.org/10.2147/IJN.S272532
Publication status	Published (in print/issue) - 22 Oct 2020

Data Access Statement

All data are available from the corresponding author upon request.

Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Keywords

Blastocystis hominis
eosin brilliant cresyl blue stain
metronidazole
Silver nanoparticles

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2147/IJN.S272532

In vitro effect of Silver nanoparticlesFinal published version, 1.6 MBLicence: CC BY-NC

Cite this

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title = "In vitro Effect of Silver Nanoparticles on Blastocystis hominis",

abstract = "Introduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis. Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs. Results: Blastocystis hominis count was reduced by 20.72\%, 28.23\%, and 18.92\% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49\%, 61.61\%, and 40.78\% after 2 hours and by 71.69\%, 79.67\%, and 62.65\% after 3 hours of adding the drugs in the same order, respectively. Conclusion: There was a statistically significant difference (P<0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.",

keywords = "Blastocystis hominis, eosin brilliant cresyl blue stain, metronidazole, Silver nanoparticles",

author = "Younis, \{Mohamed Saad\} and Abououf, \{Eman Abd El Rahman\} and Ali, \{Ali El Saeed\} and \{Abd Elhady\}, \{Sara Mohamed\} and Wassef, \{Rita Maher\}",

year = "2020",

month = oct,

day = "22",

doi = "10.2147/IJN.S272532",

language = "English",

volume = "15",

pages = "8167--8173",

journal = "International Journal of Nanomedicine",

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T1 - In vitro Effect of Silver Nanoparticles on Blastocystis hominis

AU - Younis, Mohamed Saad

AU - Abououf, Eman Abd El Rahman

AU - Ali, Ali El Saeed

AU - Abd Elhady, Sara Mohamed

AU - Wassef, Rita Maher

PY - 2020/10/22

Y1 - 2020/10/22

N2 - Introduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis. Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs. Results: Blastocystis hominis count was reduced by 20.72%, 28.23%, and 18.92% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49%, 61.61%, and 40.78% after 2 hours and by 71.69%, 79.67%, and 62.65% after 3 hours of adding the drugs in the same order, respectively. Conclusion: There was a statistically significant difference (P<0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.

AB - Introduction: This study aims to assess the efficacy of silver nanoparticles (Ag Nps) alone and combined with metronidazole (Ag Nps + MTZ) as potential alternative therapeutic agents for Blastocystis hominis. Methods: The parasites were challenged with Ag Nps, Ag Nps + MTZ and MTZ. To assess the efficacy of drugs, counting of viable parasites was done after 1, 2, and 3 hours of adding the drugs. Results: Blastocystis hominis count was reduced by 20.72%, 28.23%, and 18.92% after one hour of adding Ag Nps, Ag Nps + MTZ, and MTZ, respectively. Cysts count was further reduced by 51.49%, 61.61%, and 40.78% after 2 hours and by 71.69%, 79.67%, and 62.65% after 3 hours of adding the drugs in the same order, respectively. Conclusion: There was a statistically significant difference (P<0.05) in the in vitro growth inhibition of the parasite over the different time intervals when using the tested drugs against the control drug.

KW - Blastocystis hominis

KW - eosin brilliant cresyl blue stain

KW - metronidazole

KW - Silver nanoparticles

UR - https://www.dovepress.com/in-vitro-effect-of-silver-nanoparticles-on-blastocystis-hominis-peer-reviewed-article-IJN

UR - https://www.scopus.com/pages/publications/85094593489

U2 - 10.2147/IJN.S272532

DO - 10.2147/IJN.S272532

M3 - Article

SN - 1176-9114

VL - 15

SP - 8167

EP - 8173

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

ER -

In vitro Effect of Silver Nanoparticles on Blastocystis hominis

Abstract

Data Access Statement

Funding

Keywords

UN SDGs

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