In vitro cleavage of the carbon-phosphorus bond of phosphonopyruvate by cell extracts of an environmental Burkholderia cepacia isolate

Nigel Ternan, JP Quinn

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Cell-free extracts of Burkholderia cepacia strain Pal6 catalysed the degradation of 3-phosphonopyruvate to pyruvate and inorganic phosphate; the products were detected in equimolar quantities. The stable in vitro activity responsible was distinct from both phosphonoactealdehyde hydrolase and phosphonoacetate hydrolase and from phosphoenolpyruvate phosphomutase and appears to represent a novel mode of carbon-phosphorus bond cleavage. (C) 1998 Academic Press.
LanguageEnglish
Pages378-381
JournalBiochemical and Biophysical Research Communications
Volume248
Issue number2
DOIs
Publication statusPublished - Jul 1998

Fingerprint

Burkholderia cepacia
Hydrolases
Cell Extracts
Pyruvic Acid
Phosphorus
Carbon
Phosphates
In Vitro Techniques
phosphoenolpyruvate mutase
phosphonoacetate hydrolase
3-phosphonopyruvate

Cite this

@article{6e42187691e54c4a98c0348f5d442cdc,
title = "In vitro cleavage of the carbon-phosphorus bond of phosphonopyruvate by cell extracts of an environmental Burkholderia cepacia isolate",
abstract = "Cell-free extracts of Burkholderia cepacia strain Pal6 catalysed the degradation of 3-phosphonopyruvate to pyruvate and inorganic phosphate; the products were detected in equimolar quantities. The stable in vitro activity responsible was distinct from both phosphonoactealdehyde hydrolase and phosphonoacetate hydrolase and from phosphoenolpyruvate phosphomutase and appears to represent a novel mode of carbon-phosphorus bond cleavage. (C) 1998 Academic Press.",
author = "Nigel Ternan and JP Quinn",
year = "1998",
month = "7",
doi = "10.1006/bbrc.1998.8962",
language = "English",
volume = "248",
pages = "378--381",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - In vitro cleavage of the carbon-phosphorus bond of phosphonopyruvate by cell extracts of an environmental Burkholderia cepacia isolate

AU - Ternan, Nigel

AU - Quinn, JP

PY - 1998/7

Y1 - 1998/7

N2 - Cell-free extracts of Burkholderia cepacia strain Pal6 catalysed the degradation of 3-phosphonopyruvate to pyruvate and inorganic phosphate; the products were detected in equimolar quantities. The stable in vitro activity responsible was distinct from both phosphonoactealdehyde hydrolase and phosphonoacetate hydrolase and from phosphoenolpyruvate phosphomutase and appears to represent a novel mode of carbon-phosphorus bond cleavage. (C) 1998 Academic Press.

AB - Cell-free extracts of Burkholderia cepacia strain Pal6 catalysed the degradation of 3-phosphonopyruvate to pyruvate and inorganic phosphate; the products were detected in equimolar quantities. The stable in vitro activity responsible was distinct from both phosphonoactealdehyde hydrolase and phosphonoacetate hydrolase and from phosphoenolpyruvate phosphomutase and appears to represent a novel mode of carbon-phosphorus bond cleavage. (C) 1998 Academic Press.

U2 - 10.1006/bbrc.1998.8962

DO - 10.1006/bbrc.1998.8962

M3 - Article

VL - 248

SP - 378

EP - 381

JO - Biochemical and Biophysical Research Communications

T2 - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -