In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices

MJ Davies, Christopher Mitchell, MAL Maley, MD Grounds, AR Harvey, GW Plant, DJ Wood, Y Hong, TV Chirila

Research output: Contribution to journalArticle

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Abstract

The kinetics of controlled release of basic fibroblast growth factor (bFGF) from polymers (sutures, polycarbonate, Hydron, and Elvax), biopolymers (alginate), and biomatrices (lens capsules), and conditions for storage of bFGF (temperature, plastic type, heparin) were evaluated in vitro. Tissue culture proliferation bioassays with 3T3 fibroblasts, showed that only lens capsules with bFGF had a sustained release of bFGF for up to three weeks. The other materials released all of the `bound' bFGF with two hours or produced an inflammatory response in vivo. Therefore, the lens tissue had the most potential for controlled long-term delivery of bFGF in vivo. These studies emphasise the importance of in vitro analysis of release kinetics of growth factors from a range of materials as a basis for potential in vivo applications.
LanguageEnglish
Pages31-56
JournalJournal of Biomaterials Applications
Volume12
Issue number1
Publication statusPublished - Jul 1997

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Fibroblast Growth Factor 2
Polymers
Lenses
polycarbonate
Capsules
Biopolymers
Biological Assay
Sutures
Plastics
Heparin
In Vitro Techniques
Intercellular Signaling Peptides and Proteins
Fibroblasts
Temperature

Cite this

Davies, MJ ; Mitchell, Christopher ; Maley, MAL ; Grounds, MD ; Harvey, AR ; Plant, GW ; Wood, DJ ; Hong, Y ; Chirila, TV. / In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices. In: Journal of Biomaterials Applications. 1997 ; Vol. 12, No. 1. pp. 31-56.
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Davies, MJ, Mitchell, C, Maley, MAL, Grounds, MD, Harvey, AR, Plant, GW, Wood, DJ, Hong, Y & Chirila, TV 1997, 'In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices', Journal of Biomaterials Applications, vol. 12, no. 1, pp. 31-56.

In vitro assessment of the biological activity of basic fibroblast growth factor released from various polymers and biomatrices. / Davies, MJ; Mitchell, Christopher; Maley, MAL; Grounds, MD; Harvey, AR; Plant, GW; Wood, DJ; Hong, Y; Chirila, TV.

In: Journal of Biomaterials Applications, Vol. 12, No. 1, 07.1997, p. 31-56.

Research output: Contribution to journalArticle

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