In Vitro Antioxidant Activity and Antigenotoxic Effects of Avenanthramides and Related Compounds

Alison M. Lee-Manion, Ruth K. Price, JJ Strain, Lena H. Dimberg, Kerstin Sunnerheim, RW Welch

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Avenanthramides are substituted N-cinnamoylanthranilic acids, with hydroxycinnamic acid and anthranilic acid moieties. These alkaloid phenols, which are unique to oats, may confer health benefits via antioxidant or other mechanisms. Synthetic avenanthramides, hydroxycinnamic acids, Tranilast, and ascorbic acid were evaluated for antioxidant activity using two assays, DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant potential), and for antigenotoxicity using the Comet assay with stressed human adenocarcinoma colon cells. Of all the compounds tested, N-(3',4'-dihydroxy-(E)-cinnamoyl)-5-hydroxyanthranilic acid (2c), an abundant oat avenanthramide, generally had the highest activity in all three assays. The drug Tranilast showed antigenotoxic effects, but not antioxidant activity, suggesting that antigenotoxicity is not dependent on antioxidant effects. Overall, results show that avenanthramides exert antioxidant and antigenotoxic activities that are comparable to those of ascorbic acid and which have the potential to exert beneficial physiological effects.
Original languageEnglish
Pages (from-to)10619-10624
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number22
DOIs
Publication statusPublished - Nov 2009

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antioxidant activity
antioxidants
coumaric acids
oats
hydroxyanthranilic acid
assays
ascorbic acid
anthranilic acid
adenocarcinoma
phenols
colon
alkaloids
drugs
avenanthramides
acids
cells

Cite this

Lee-Manion, Alison M. ; Price, Ruth K. ; Strain, JJ ; Dimberg, Lena H. ; Sunnerheim, Kerstin ; Welch, RW. / In Vitro Antioxidant Activity and Antigenotoxic Effects of Avenanthramides and Related Compounds. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 22. pp. 10619-10624.
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In Vitro Antioxidant Activity and Antigenotoxic Effects of Avenanthramides and Related Compounds. / Lee-Manion, Alison M.; Price, Ruth K.; Strain, JJ; Dimberg, Lena H.; Sunnerheim, Kerstin; Welch, RW.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 22, 11.2009, p. 10619-10624.

Research output: Contribution to journalArticle

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AB - Avenanthramides are substituted N-cinnamoylanthranilic acids, with hydroxycinnamic acid and anthranilic acid moieties. These alkaloid phenols, which are unique to oats, may confer health benefits via antioxidant or other mechanisms. Synthetic avenanthramides, hydroxycinnamic acids, Tranilast, and ascorbic acid were evaluated for antioxidant activity using two assays, DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant potential), and for antigenotoxicity using the Comet assay with stressed human adenocarcinoma colon cells. Of all the compounds tested, N-(3',4'-dihydroxy-(E)-cinnamoyl)-5-hydroxyanthranilic acid (2c), an abundant oat avenanthramide, generally had the highest activity in all three assays. The drug Tranilast showed antigenotoxic effects, but not antioxidant activity, suggesting that antigenotoxicity is not dependent on antioxidant effects. Overall, results show that avenanthramides exert antioxidant and antigenotoxic activities that are comparable to those of ascorbic acid and which have the potential to exert beneficial physiological effects.

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