Improved antioxidative defence protects insulin-producing cells against homocysteine toxicity

SM Scullion, C Hahn, K Tyka, Peter Flatt, Neville McClenaghan, S Lenzen, E Gurgul-Convey

Research output: Contribution to journalArticle

Abstract

Homocysteine (HC) is considered to play an important role in the development of metabolic syndrome complications. Insulin-producing cells are prone to HC toxicity and this has been linked to oxidative stress. However, the exact mechanisms remain unknown. Therefore it was the aim of this study to determine the nature of reactive oxygen species responsible for HC toxicity.Chronic exposure of RINm5F and INS1E insulin-producing cells to HC decreased cell viability and glucose-induced insulin secretion in a concentration-dependent manner and led to a significant induction of hydrogen peroxide generation in the cytosolic, but not the mitochondrial compartment of the cell. Cytosolic overexpression of catalase, a hydrogen peroxide detoxifying enzyme, provided a significant protection against viability loss and hydrogen peroxide generation,while mitochondrial overexpression of catalase did not protect against HC toxicity. Overexpression of CuZnSOD, a cytosolic superoxide dismutating enzyme, also protected against HC toxicity. However, the best protection was achieved in the case of a combined overexpression of CuZnSOD and catalase. Incubation of cells in combination with alloxan resulted in a significant increase of HC toxicity and an increase of hydrogen peroxide generation. Overexpression ofCuZnSOD or catalase protected against the toxicity of HC plus alloxan, with a superior protection achieved again by combined overexpression. The results indicate that HC induces oxidative stress in insulin-producing cells by stimulation of superoxide radical and hydrogen peroxide generation in the cytoplasm. The low antioxidative defence status makes the insulin-producing cells very vulnerable to HC toxicity.
LanguageEnglish
Pages37-46
JournalChemico-Biological Interactions
Volume256
Early online date16 Jun 2016
DOIs
Publication statusE-pub ahead of print - 16 Jun 2016

Fingerprint

Homocysteine
Toxicity
Insulin
Hydrogen Peroxide
Catalase
Alloxan
Oxidative stress
Superoxides
Oxidative Stress
Cells
Enzymes
Reactive Oxygen Species
Cell Survival
Cytoplasm
Glucose

Keywords

  • Homocysteine
  • Insulin-producing cells
  • Antioxidant enzymes
  • Oxidative stress
  • Alloxan
  • Diabetes

Cite this

Scullion, SM ; Hahn, C ; Tyka, K ; Flatt, Peter ; McClenaghan, Neville ; Lenzen, S ; Gurgul-Convey, E. / Improved antioxidative defence protects insulin-producing cells against homocysteine toxicity. In: Chemico-Biological Interactions. 2016 ; Vol. 256. pp. 37-46.
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Improved antioxidative defence protects insulin-producing cells against homocysteine toxicity. / Scullion, SM; Hahn, C; Tyka, K; Flatt, Peter; McClenaghan, Neville; Lenzen, S; Gurgul-Convey, E.

In: Chemico-Biological Interactions, Vol. 256, 16.06.2016, p. 37-46.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Improved antioxidative defence protects insulin-producing cells against homocysteine toxicity

AU - Scullion, SM

AU - Hahn, C

AU - Tyka, K

AU - Flatt, Peter

AU - McClenaghan, Neville

AU - Lenzen, S

AU - Gurgul-Convey, E

N1 - Compliant in UIR; evidence uploaded to 'Other files'

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Y1 - 2016/6/16

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AB - Homocysteine (HC) is considered to play an important role in the development of metabolic syndrome complications. Insulin-producing cells are prone to HC toxicity and this has been linked to oxidative stress. However, the exact mechanisms remain unknown. Therefore it was the aim of this study to determine the nature of reactive oxygen species responsible for HC toxicity.Chronic exposure of RINm5F and INS1E insulin-producing cells to HC decreased cell viability and glucose-induced insulin secretion in a concentration-dependent manner and led to a significant induction of hydrogen peroxide generation in the cytosolic, but not the mitochondrial compartment of the cell. Cytosolic overexpression of catalase, a hydrogen peroxide detoxifying enzyme, provided a significant protection against viability loss and hydrogen peroxide generation,while mitochondrial overexpression of catalase did not protect against HC toxicity. Overexpression of CuZnSOD, a cytosolic superoxide dismutating enzyme, also protected against HC toxicity. However, the best protection was achieved in the case of a combined overexpression of CuZnSOD and catalase. Incubation of cells in combination with alloxan resulted in a significant increase of HC toxicity and an increase of hydrogen peroxide generation. Overexpression ofCuZnSOD or catalase protected against the toxicity of HC plus alloxan, with a superior protection achieved again by combined overexpression. The results indicate that HC induces oxidative stress in insulin-producing cells by stimulation of superoxide radical and hydrogen peroxide generation in the cytoplasm. The low antioxidative defence status makes the insulin-producing cells very vulnerable to HC toxicity.

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