Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide

Ece Yarali; Ece Eksin; Hilal Torul; Abhijit Ganguly; Ugur Tamer; Pagona Papakonstantinou; Arzum Erdem

doi:10.1016/j.talanta.2022.123233

Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide

Ece Yarali, Ece Eksin, Hilal Torul, Abhijit Ganguly, Ugur Tamer, Pagona Papakonstantinou, Arzum Erdem

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Abstract

Paper-based electrodes modified with molybdenum disulfide (MoS2) in the form of bulk crystals or exfoliated nanosheets were developed and used as a biosensing platform for the impedimetric detection of miRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. For this purpose, MoS2 crystals or nanosheets were used for the modification of the working electrode area of paper-based platform for the first time in this study. The proposed assay offers sensitive and selective detection of microRNAs by electrochemical impedance spectroscopy (EIS) technique. The entire assay, both the electrode modification and the miRNA detection being completed in 30 min and a single sample droplet (5 μL) was enough to cover working electrode area which enabled analysis in low sample volumes. The limits of detection (LOD) for miRNA-21 and miRNA-155 were calculated both in buffer and fetal bovine serum media. It is found that the LOD is varying between 1 and 200 ng/mL. In comparison to nanosheets, a larger electroactive surface area was obtained with bulk MoS2 resulting in lower LOD values on miRNA detection. The paper-based electrodes showed high specificity towards their target sequences. Moreover, they effectively discriminated single base mismatched non-target sequences. The advantages of these MoS2 paper based electrodes include high sensitivity, and low-cost provide great potential for improved monitoring of miRNA biomarkers even in artificial serum media.

Original language	English
Article number	123233
Pages (from-to)	123233
Journal	Talanta
Volume	241
Early online date	16 Jan 2022
DOIs	https://doi.org/10.1016/j.talanta.2022.123233
Publication status	Published (in print/issue) - 1 May 2022

Bibliographical note

Funding Information:
This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council ( TUBITAK ; Project no. 215Z702 ), the British Council (Newton Fund, Institutional Links, Ref: 216182787 ) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 .

Funding Information:
This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702 ) and the British Council (Newton Fund, Institutional Links, Ref: 216182787 ). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E., master's students and PhD, respectively, acknowledge a project scholarship ( TUBITAK Project no. 215Z702 ). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project ( TUBITAK ; Project no. 215Z702 ). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 .

Funding Information:
This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702), the British Council (Newton Fund, Institutional Links, Ref: 216182787) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702) and the British Council (Newton Fund, Institutional Links, Ref: 216182787). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E. master's students and PhD, respectively, acknowledge a project scholarship (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.

Publisher Copyright:
© 2022 Elsevier B.V.

Copyright © 2022 Elsevier B.V. All rights reserved.

Keywords

Electrochemical impedance spectroscopy
microRNA
Molybdenum disulfide
Paper-based electrode
Electrodes
Limit of Detection
MicroRNAs/analysis
Biosensing Techniques/methods
Disulfides/chemistry
Biomarkers
Molybdenum/chemistry
Electrochemical Techniques/methods

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.talanta.2022.123233

revised_ miRNA TALANTA_Manuscript_Author Accepted Manuscript, 1.32 MBLicence: CC BY-NC-ND
Supplementary MaterialLicence: CC BY-NC-ND

Cite this

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title = "Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide",

abstract = "Paper-based electrodes modified with molybdenum disulfide (MoS2) in the form of bulk crystals or exfoliated nanosheets were developed and used as a biosensing platform for the impedimetric detection of miRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. For this purpose, MoS2 crystals or nanosheets were used for the modification of the working electrode area of paper-based platform for the first time in this study. The proposed assay offers sensitive and selective detection of microRNAs by electrochemical impedance spectroscopy (EIS) technique. The entire assay, both the electrode modification and the miRNA detection being completed in 30 min and a single sample droplet (5 μL) was enough to cover working electrode area which enabled analysis in low sample volumes. The limits of detection (LOD) for miRNA-21 and miRNA-155 were calculated both in buffer and fetal bovine serum media. It is found that the LOD is varying between 1 and 200 ng/mL. In comparison to nanosheets, a larger electroactive surface area was obtained with bulk MoS2 resulting in lower LOD values on miRNA detection. The paper-based electrodes showed high specificity towards their target sequences. Moreover, they effectively discriminated single base mismatched non-target sequences. The advantages of these MoS2 paper based electrodes include high sensitivity, and low-cost provide great potential for improved monitoring of miRNA biomarkers even in artificial serum media.",

keywords = "Electrochemical impedance spectroscopy, microRNA, Molybdenum disulfide, Paper-based electrode, Electrodes, Limit of Detection, MicroRNAs/analysis, Biosensing Techniques/methods, Disulfides/chemistry, Biomarkers, Molybdenum/chemistry, Electrochemical Techniques/methods",

author = "Ece Yarali and Ece Eksin and Hilal Torul and Abhijit Ganguly and Ugur Tamer and Pagona Papakonstantinou and Arzum Erdem",

note = "Funding Information: This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council ( TUBITAK ; Project no. 215Z702 ), the British Council (Newton Fund, Institutional Links, Ref: 216182787 ) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 . Funding Information: This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702 ) and the British Council (Newton Fund, Institutional Links, Ref: 216182787 ). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E., master's students and PhD, respectively, acknowledge a project scholarship ( TUBITAK Project no. 215Z702 ). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project ( TUBITAK ; Project no. 215Z702 ). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 . Funding Information: This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702), the British Council (Newton Fund, Institutional Links, Ref: 216182787) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702) and the British Council (Newton Fund, Institutional Links, Ref: 216182787). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E. master's students and PhD, respectively, acknowledge a project scholarship (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186. Publisher Copyright: {\textcopyright} 2022 Elsevier B.V. Copyright {\textcopyright} 2022 Elsevier B.V. All rights reserved.",

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AU - Eksin, Ece

AU - Torul, Hilal

AU - Ganguly, Abhijit

AU - Tamer, Ugur

AU - Papakonstantinou, Pagona

AU - Erdem, Arzum

N1 - Funding Information: This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council ( TUBITAK ; Project no. 215Z702 ), the British Council (Newton Fund, Institutional Links, Ref: 216182787 ) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 . Funding Information: This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702 ) and the British Council (Newton Fund, Institutional Links, Ref: 216182787 ). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E., master's students and PhD, respectively, acknowledge a project scholarship ( TUBITAK Project no. 215Z702 ). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project ( TUBITAK ; Project no. 215Z702 ). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186 . Funding Information: This project was supported by the Newton-Katip Celebi funding program, and received a financial support from the Turkish Scientific and Technological Research Council (TUBITAK; Project no. 215Z702), the British Council (Newton Fund, Institutional Links, Ref: 216182787) and Invest Northern Ireland under a Biodevices grant, Ref. RD0714186.This project was supported by the Newton-Katip Celebi funding program, and authors acknowledge financial support from the Turkish Scientific and Technological Research Council TUBITAK Project no. 215Z702) and the British Council (Newton Fund, Institutional Links, Ref: 216182787). A.E. would also like to express her gratitude to the Turkish Academy of Sciences (TUBA) as a principal member for its partial support. E.Y. and E.E. master's students and PhD, respectively, acknowledge a project scholarship (TUBITAK Project no. 215Z702). Authors also acknowledge to helpful discussion of Assoc. Prof. Yildiz Uludag as the project consultant during project (TUBITAK; Project no. 215Z702). P.P. acknowledges support from Invest Northern Ireland under a Biodevices grant, Ref. RD0714186. Publisher Copyright: © 2022 Elsevier B.V. Copyright © 2022 Elsevier B.V. All rights reserved.

PY - 2022/5/1

Y1 - 2022/5/1

N2 - Paper-based electrodes modified with molybdenum disulfide (MoS2) in the form of bulk crystals or exfoliated nanosheets were developed and used as a biosensing platform for the impedimetric detection of miRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. For this purpose, MoS2 crystals or nanosheets were used for the modification of the working electrode area of paper-based platform for the first time in this study. The proposed assay offers sensitive and selective detection of microRNAs by electrochemical impedance spectroscopy (EIS) technique. The entire assay, both the electrode modification and the miRNA detection being completed in 30 min and a single sample droplet (5 μL) was enough to cover working electrode area which enabled analysis in low sample volumes. The limits of detection (LOD) for miRNA-21 and miRNA-155 were calculated both in buffer and fetal bovine serum media. It is found that the LOD is varying between 1 and 200 ng/mL. In comparison to nanosheets, a larger electroactive surface area was obtained with bulk MoS2 resulting in lower LOD values on miRNA detection. The paper-based electrodes showed high specificity towards their target sequences. Moreover, they effectively discriminated single base mismatched non-target sequences. The advantages of these MoS2 paper based electrodes include high sensitivity, and low-cost provide great potential for improved monitoring of miRNA biomarkers even in artificial serum media.

AB - Paper-based electrodes modified with molybdenum disulfide (MoS2) in the form of bulk crystals or exfoliated nanosheets were developed and used as a biosensing platform for the impedimetric detection of miRNAs (miRNA-155 and miRNA-21) related to early diagnosis of lung cancer. For this purpose, MoS2 crystals or nanosheets were used for the modification of the working electrode area of paper-based platform for the first time in this study. The proposed assay offers sensitive and selective detection of microRNAs by electrochemical impedance spectroscopy (EIS) technique. The entire assay, both the electrode modification and the miRNA detection being completed in 30 min and a single sample droplet (5 μL) was enough to cover working electrode area which enabled analysis in low sample volumes. The limits of detection (LOD) for miRNA-21 and miRNA-155 were calculated both in buffer and fetal bovine serum media. It is found that the LOD is varying between 1 and 200 ng/mL. In comparison to nanosheets, a larger electroactive surface area was obtained with bulk MoS2 resulting in lower LOD values on miRNA detection. The paper-based electrodes showed high specificity towards their target sequences. Moreover, they effectively discriminated single base mismatched non-target sequences. The advantages of these MoS2 paper based electrodes include high sensitivity, and low-cost provide great potential for improved monitoring of miRNA biomarkers even in artificial serum media.

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KW - Electrochemical Techniques/methods

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DO - 10.1016/j.talanta.2022.123233

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SN - 0039-9140

VL - 241

SP - 123233

JO - Talanta

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Impedimetric detection of miRNA biomarkers using paper-based electrodes modified with bulk crystals or nanosheets of molybdenum disulfide

Abstract

Bibliographical note

Keywords

UN SDGs

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