Impact of a polyphenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight/obese population: a randomised controlled trial.

FR Baldrick, Kevin McFadden, Maria Ibars, Chris Sung, Tanya Moffat, Kate Megarry, Keith Thomas, P Mitchell, JULIE WALLACE, L. Kirsty Pourshahidi, Nigel G Ternan, Giulia Corona, Jeremy Spencer, Parveen Yahoob, Sarah Hotchkiss, Ross Campbell, Jose manuel Moreno-Rojas, Francisco Julián Cuevas, Gema Pereira-Caro, Ian Rowland & 1 others Chris IR Gill

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Epidemiological evidence suggests a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenols
Objective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract from on DNA damage, oxidative stress, and inflammation in vivo.
Design: A randomised double-blind placebo-controlled crossover trial was conducted in 80 participants aged 30-65 years with a BMI ≥25kg/m2. The participants consumed either a 400 mg capsule containing 100 mg of seaweed (poly)phenol and 300 mg maltodextrin or a 400 mg maltodextrin placebo control capsule daily for an 8-week period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples following seaweed consumption was determined by UHPLC-HR-MS.
Results: Consumption of the seaweed (poly)phenols resulted in a modest decrease DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimers of phloroglucinol sulfate, C-O-C dimers of phloroglucinol and diphlorethol sulfate.
Conclusion: To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population, the subgroup analysis should be considered exploratory as it was not preplanned; therefore, are not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies.
LanguageEnglish
Pages688-700
JournalAmerican Journal of Clinical Nutrition
Volume108
Issue number4
Early online date1 Oct 2018
DOIs
Publication statusPublished - 12 Oct 2018

Fingerprint

Ascophyllum
Phaeophyta
Seaweed
Polyphenols
DNA Damage
Randomized Controlled Trials
Antioxidants
Phloroglucinol
Population
Sulfates
Biological Availability
Phenols
Glucuronides
Phenol
Capsules
Biomarkers
Placebos
Pyrogallol
Cytokines
Metabolomics

Keywords

  • Seaweed
  • bioavailability
  • Oxidative stress
  • DNA damage
  • Inflammation
  • Phenolic compounds

Cite this

Baldrick, FR ; McFadden, Kevin ; Ibars, Maria ; Sung, Chris ; Moffat, Tanya ; Megarry, Kate ; Thomas, Keith ; Mitchell, P ; WALLACE, JULIE ; Pourshahidi, L. Kirsty ; Ternan, Nigel G ; Corona, Giulia ; Spencer, Jeremy ; Yahoob, Parveen ; Hotchkiss, Sarah ; Campbell, Ross ; Moreno-Rojas, Jose manuel ; Cuevas, Francisco Julián ; Pereira-Caro, Gema ; Rowland, Ian ; Gill, Chris IR. / Impact of a polyphenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight/obese population: a randomised controlled trial. In: American Journal of Clinical Nutrition. 2018 ; Vol. 108, No. 4. pp. 688-700.
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abstract = "Background: Epidemiological evidence suggests a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenolsObjective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract from on DNA damage, oxidative stress, and inflammation in vivo.Design: A randomised double-blind placebo-controlled crossover trial was conducted in 80 participants aged 30-65 years with a BMI ≥25kg/m2. The participants consumed either a 400 mg capsule containing 100 mg of seaweed (poly)phenol and 300 mg maltodextrin or a 400 mg maltodextrin placebo control capsule daily for an 8-week period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples following seaweed consumption was determined by UHPLC-HR-MS. Results: Consumption of the seaweed (poly)phenols resulted in a modest decrease DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimers of phloroglucinol sulfate, C-O-C dimers of phloroglucinol and diphlorethol sulfate. Conclusion: To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population, the subgroup analysis should be considered exploratory as it was not preplanned; therefore, are not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies.",
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Baldrick, FR, McFadden, K, Ibars, M, Sung, C, Moffat, T, Megarry, K, Thomas, K, Mitchell, P, WALLACE, JULIE, Pourshahidi, LK, Ternan, NG, Corona, G, Spencer, J, Yahoob, P, Hotchkiss, S, Campbell, R, Moreno-Rojas, JM, Cuevas, FJ, Pereira-Caro, G, Rowland, I & Gill, CIR 2018, 'Impact of a polyphenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight/obese population: a randomised controlled trial.', American Journal of Clinical Nutrition, vol. 108, no. 4, pp. 688-700. https://doi.org/10.1093/ajcn/nqy147

Impact of a polyphenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight/obese population: a randomised controlled trial. / Baldrick, FR; McFadden, Kevin; Ibars, Maria; Sung, Chris; Moffat, Tanya ; Megarry, Kate ; Thomas, Keith; Mitchell, P; WALLACE, JULIE; Pourshahidi, L. Kirsty; Ternan, Nigel G; Corona, Giulia; Spencer, Jeremy; Yahoob, Parveen; Hotchkiss, Sarah; Campbell, Ross; Moreno-Rojas, Jose manuel; Cuevas, Francisco Julián; Pereira-Caro, Gema; Rowland, Ian ; Gill, Chris IR.

In: American Journal of Clinical Nutrition, Vol. 108, No. 4, 12.10.2018, p. 688-700.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Impact of a polyphenol-rich extract from the brown algae Ascophyllum nodosum on DNA damage and antioxidant activity in an overweight/obese population: a randomised controlled trial.

AU - Baldrick, FR

AU - McFadden, Kevin

AU - Ibars, Maria

AU - Sung, Chris

AU - Moffat, Tanya

AU - Megarry, Kate

AU - Thomas, Keith

AU - Mitchell, P

AU - WALLACE, JULIE

AU - Pourshahidi, L. Kirsty

AU - Ternan, Nigel G

AU - Corona, Giulia

AU - Spencer, Jeremy

AU - Yahoob, Parveen

AU - Hotchkiss, Sarah

AU - Campbell, Ross

AU - Moreno-Rojas, Jose manuel

AU - Cuevas, Francisco Julián

AU - Pereira-Caro, Gema

AU - Rowland, Ian

AU - Gill, Chris IR

PY - 2018/10/12

Y1 - 2018/10/12

N2 - Background: Epidemiological evidence suggests a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenolsObjective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract from on DNA damage, oxidative stress, and inflammation in vivo.Design: A randomised double-blind placebo-controlled crossover trial was conducted in 80 participants aged 30-65 years with a BMI ≥25kg/m2. The participants consumed either a 400 mg capsule containing 100 mg of seaweed (poly)phenol and 300 mg maltodextrin or a 400 mg maltodextrin placebo control capsule daily for an 8-week period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples following seaweed consumption was determined by UHPLC-HR-MS. Results: Consumption of the seaweed (poly)phenols resulted in a modest decrease DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimers of phloroglucinol sulfate, C-O-C dimers of phloroglucinol and diphlorethol sulfate. Conclusion: To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population, the subgroup analysis should be considered exploratory as it was not preplanned; therefore, are not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies.

AB - Background: Epidemiological evidence suggests a diet rich in (poly)phenols has beneficial effects on many chronic diseases. Brown seaweed is a rich source of (poly)phenolsObjective: The aim of this study was to investigate the bioavailability and effect of a brown seaweed (Ascophyllum nodosum) (poly)phenol extract from on DNA damage, oxidative stress, and inflammation in vivo.Design: A randomised double-blind placebo-controlled crossover trial was conducted in 80 participants aged 30-65 years with a BMI ≥25kg/m2. The participants consumed either a 400 mg capsule containing 100 mg of seaweed (poly)phenol and 300 mg maltodextrin or a 400 mg maltodextrin placebo control capsule daily for an 8-week period. Bioactivity was assessed with a panel of blood-based markers including lymphocyte DNA damage, plasma oxidant capacity, C-reactive protein and inflammatory cytokines. To explore the bioavailability of seaweed phenolics, an untargeted metabolomics analysis of urine and plasma samples following seaweed consumption was determined by UHPLC-HR-MS. Results: Consumption of the seaweed (poly)phenols resulted in a modest decrease DNA damage but only in a subset of the total population who were obese. There were no significant changes in CRP, antioxidant status, inflammatory cytokines. We identified phlorotannin metabolites that are considered potential biomarkers of seaweed consumption including pyrogallol/phloroglucinol-sulfate, hydroxytrifurahol A-glucuronide, dioxinodehydroeckol-glucuronide, diphlorethol sulfates, C-O-C dimers of phloroglucinol sulfate, C-O-C dimers of phloroglucinol and diphlorethol sulfate. Conclusion: To the best of our knowledge, this work represents the first comprehensive study investigating the bioactivity and bioavailability of seaweed (poly)phenolics in human participants. We identified several potential biomarkers of seaweed consumption. Intriguingly, the modest improvements in DNA damage were observed only in the obese subset of the total population, the subgroup analysis should be considered exploratory as it was not preplanned; therefore, are not powered adequately. Elucidation of the biology underpinning this observation will require participant stratification according to weight in future studies.

KW - Seaweed

KW - bioavailability

KW - Oxidative stress

KW - DNA damage

KW - Inflammation

KW - Phenolic compounds

U2 - 10.1093/ajcn/nqy147

DO - 10.1093/ajcn/nqy147

M3 - Article

VL - 108

SP - 688

EP - 700

JO - American Journal of Clinical Nutrition

T2 - American Journal of Clinical Nutrition

JF - American Journal of Clinical Nutrition

SN - 0002-9165

IS - 4

ER -