Identification and cloning of four riboswitches from Burkholderia pseudomallei strain K96243

Noor Munyati-Othman, Ahmad Luqman Abdul Fatah, Mohd Al Akmarul Fizree Bin Md Piji, E. Ramlan, Mohd Firdaus Raih

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Structured RNAs referred as riboswitches have been predicted to be present in the genome sequence of Burkholderia pseudomallei strain K96243. Four of the riboswitches were identified and analyzed through BLASTN, Rfam search and multiple sequence alignment. The RNA aptamers belong to the following riboswitch classifications: glycine riboswitch, cobalamin riboswitch, S-adenosyl-(L)-homocysteine (SAH) riboswitch and flavin mononucleotide (FMN) riboswitch. The conserved nucleotides for each aptamer were identified and were marked on the secondary structure generated by RNAfold. These riboswitches were successfully amplified and cloned for further study.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
EditorsMohd Shahrul Mohd Nadzir, Humaida Banu Samsudin, Marlia Mohd Hanafiah, Asmat Ahmad, Nurul Izzaty Hassan, Izfa Riza Hazmi, Hani Kartini Agustar, Zahari Ibarahim, Noor Baa'yah Ibrahim, Sakhinah Abu Bakar, Mohd Salmi Md Noorani, Nurul Huda Abd Karim, Saiful Irwan Zubairi, Nazlina Ibrahim
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Conference

Conference2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/04/1516/04/15

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