Hydrolysis of olive mill waste to enhance rhamnolipids and surfactin production

IM Ramírez, DA Vaz, Ibrahim M Banat, Roger Marchant, EJ Alameda, MG Román

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The aim of this work was to demonstrate the effectiveness of hydrolysis pretreatment of olive mill (OMW) waste before use as a carbon source in biosurfactant production by fermentation. Three hydrolysis methods were assessed: enzymatic hydrolysis, acid pretreatment plus enzymatic hydrolysis, and acid hydrolysis. Fermentation was carried out using two bacterial species: Pseudomonas aeruginosa and Bacillus subtilis. Our results showed that the enzymatic hydrolysis was the best pretreatment, yielding up to 29.5 and 13.7 mg/L of rhamnolipids and surfactins respectively. Glucose did not show significant differences in comparison to enzymatically hydrolysed OMW. At the best conditions found rhamnolipids and surfactins reached concentrations of 299 and 26.5 mg/L; values considerably higher than those obtained with non-hydrolysed OMW. In addition, enzymatic pretreatment seemed to partially reduce the inhibitory effects of OMW on surfactin production. Therefore, enzymatic hydrolysis proved to effectively increase the productivity of these biosurfactants using OMW as the sole carbon source.
LanguageEnglish
Pages1-6
JournalBioresource Technology
Volume205
Early online date13 Jan 2016
DOIs
Publication statusE-pub ahead of print - 13 Jan 2016

Fingerprint

Enzymatic hydrolysis
hydrolysis
Hydrolysis
mill
Fermentation
Carbon
Acids
Bacilli
fermentation
Glucose
Productivity
acid
carbon
rhamnolipid
glucose
productivity

Keywords

  • Hydrolysis
  • olive mill waste
  • rhamnolipids
  • surfactin
  • biosurfactanta

Cite this

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abstract = "The aim of this work was to demonstrate the effectiveness of hydrolysis pretreatment of olive mill (OMW) waste before use as a carbon source in biosurfactant production by fermentation. Three hydrolysis methods were assessed: enzymatic hydrolysis, acid pretreatment plus enzymatic hydrolysis, and acid hydrolysis. Fermentation was carried out using two bacterial species: Pseudomonas aeruginosa and Bacillus subtilis. Our results showed that the enzymatic hydrolysis was the best pretreatment, yielding up to 29.5 and 13.7 mg/L of rhamnolipids and surfactins respectively. Glucose did not show significant differences in comparison to enzymatically hydrolysed OMW. At the best conditions found rhamnolipids and surfactins reached concentrations of 299 and 26.5 mg/L; values considerably higher than those obtained with non-hydrolysed OMW. In addition, enzymatic pretreatment seemed to partially reduce the inhibitory effects of OMW on surfactin production. Therefore, enzymatic hydrolysis proved to effectively increase the productivity of these biosurfactants using OMW as the sole carbon source.",
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Hydrolysis of olive mill waste to enhance rhamnolipids and surfactin production. / Ramírez, IM; Vaz, DA; Banat, Ibrahim M; Marchant, Roger; Alameda, EJ; Román, MG.

In: Bioresource Technology, Vol. 205, 13.01.2016, p. 1-6.

Research output: Contribution to journalArticle

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AU - Ramírez, IM

AU - Vaz, DA

AU - Banat, Ibrahim M

AU - Marchant, Roger

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AU - Román, MG

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AB - The aim of this work was to demonstrate the effectiveness of hydrolysis pretreatment of olive mill (OMW) waste before use as a carbon source in biosurfactant production by fermentation. Three hydrolysis methods were assessed: enzymatic hydrolysis, acid pretreatment plus enzymatic hydrolysis, and acid hydrolysis. Fermentation was carried out using two bacterial species: Pseudomonas aeruginosa and Bacillus subtilis. Our results showed that the enzymatic hydrolysis was the best pretreatment, yielding up to 29.5 and 13.7 mg/L of rhamnolipids and surfactins respectively. Glucose did not show significant differences in comparison to enzymatically hydrolysed OMW. At the best conditions found rhamnolipids and surfactins reached concentrations of 299 and 26.5 mg/L; values considerably higher than those obtained with non-hydrolysed OMW. In addition, enzymatic pretreatment seemed to partially reduce the inhibitory effects of OMW on surfactin production. Therefore, enzymatic hydrolysis proved to effectively increase the productivity of these biosurfactants using OMW as the sole carbon source.

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