Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responseston-dodecane

Marta Barbato, Alberto Scoma, Francesca Mapelli, Rebecca DeSmet, Ibrahim M Banat, Daniele Daffonchio, Nico Boon, Sara Borin

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Autochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.
LanguageEnglish
JournalFrontiers in Microbiology
VolumeDec.
DOIs
Publication statusPublished - 21 Dec 2016

Fingerprint

Alcanivorax
transcriptomics
hydrocarbons
alkanes
Alcanivorax dieselolei
Alcanivorax borkumensis
biosurfactants
genes
carbon
surface tension
oil spills
bioremediation
hydrophobicity
marine environment
surface water
transcription (genetics)
microorganisms
genomics
oils
metabolism

Keywords

  • Alcanivorax
  • transcriptomics
  • alkanes
  • bioremediation
  • functional redundancy

Cite this

Barbato, Marta ; Scoma, Alberto ; Mapelli, Francesca ; DeSmet, Rebecca ; Banat, Ibrahim M ; Daffonchio, Daniele ; Boon, Nico ; Borin, Sara. / Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responseston-dodecane. In: Frontiers in Microbiology. 2016 ; Vol. Dec.
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abstract = "Autochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28{\%}), downregulated (37{\%}) and equally regulated (36{\%}) genes, while SK2 transcription was upregulated for most of the genes (81{\%}) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.",
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Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responseston-dodecane. / Barbato, Marta; Scoma, Alberto; Mapelli, Francesca; DeSmet, Rebecca; Banat, Ibrahim M; Daffonchio, Daniele; Boon, Nico; Borin, Sara.

In: Frontiers in Microbiology, Vol. Dec., 21.12.2016.

Research output: Contribution to journalArticle

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T1 - Hydrocarbonoclastic Alcanivorax Isolates Exhibit Different Physiological and Expression Responseston-dodecane

AU - Barbato, Marta

AU - Scoma, Alberto

AU - Mapelli, Francesca

AU - DeSmet, Rebecca

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AU - Borin, Sara

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AB - Autochthonous microorganisms inhabiting hydrocarbon polluted marine environments play a fundamental role in natural attenuation and constitute promising resources for bioremediation approaches. Alcanivorax spp. members are ubiquitous in contaminated surface waters and are the first to flourish on a wide range of alkanes after an oil-spill. Following oil contamination, a transient community of different Alcanivorax spp. develop, but whether they use a similar physiological, cellular and transcriptomic response to hydrocarbon substrates is unknown. In order to identify which cellular mechanisms are implicated in alkane degradation, we investigated the response of two isolates belonging to different Alcanivorax species, A. dieselolei KS 293 and A. borkumensis SK2 growing on n-dodecane (C12) or on pyruvate. Both strains were equally able to grow on C12 but they activated different strategies to exploit it as carbon and energy source. The membrane morphology and hydrophobicity of SK2 changed remarkably, from neat and hydrophilic on pyruvate to indented and hydrophobic on C12, while no changes were observed in KS 293. In addition, SK2 accumulated a massive amount of intracellular grains when growing on pyruvate, which might constitute a carbon reservoir. Furthermore, SK2 significantly decreased medium surface tension with respect to KS 293 when growing on C12, as a putative result of higher production of biosurfactants. The transcriptomic responses of the two isolates were also highly different. KS 293 changes were relatively balanced when growing on C12 with respect to pyruvate, giving almost the same amount of upregulated (28%), downregulated (37%) and equally regulated (36%) genes, while SK2 transcription was upregulated for most of the genes (81%) when growing on pyruvate when compared to C12. While both strains, having similar genomic background in genes related to hydrocarbon metabolism, retained the same capability to grow on C12, they nevertheless presented very different physiological, cellular and transcriptomic landscapes.

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