Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma

Vibha K. Tamboli, Nikhil Bhalla, Pawan Jolly, Chris R. Bowen, John T. Taylor, Jenna L. Bowen, Chris J. Allender, Pedro Estrela

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The study reports the use of extended gate field-effect transistors (FET) for the label-free and sensitive detection of prostate cancer (PCa) biomarkers in human plasma. The approach integrates for the first time hybrid synthetic receptors comprising of highly selective aptamer-lined pockets (apta-MIP) with FETs for sensitive detection of prostate specific antigen (PSA) at clinically relevant concentrations. The hybrid synthetic receptors were constructed by immobilizing an aptamer-PSA complex on gold and subjecting it to 13 cycles of dopamine electropolymerization. The polymerization resulted in the creation of highly selective polymeric cavities that retained the ability to recognize PSA post removal of the protein. The hybrid synthetic receptors were subsequently used in an extended gate FET setup for electrochemical detection of PSA. The sensor was reported to have a limit of detection of 0.1 pg/mL with a linear detection range from 0.1 pg/mL to 1 ng/ mL PSA. Detection of 1-10 pg/mL PSA was also achieved in diluted human plasma. The present apta-MIP sensor developed in conjunction with FET devices demonstrates the potential for clinical application of synthetic hybrid receptors for the detection of clinically relevant biomarkers in complex samples.

LanguageEnglish
Pages11486-11490
Number of pages5
JournalAnalytical Chemistry
Volume88
Issue number23
Early online date17 Nov 2016
DOIs
Publication statusPublished - 6 Dec 2016

Fingerprint

Artificial Receptors
Plasma (human)
MOSFET devices
Prostate-Specific Antigen
Gates (transistor)
Field effect transistors
Electropolymerization
Sensors
Biomarkers
Tumor Biomarkers
Gold
Labels
Dopamine
Polymerization

Cite this

Tamboli, Vibha K. ; Bhalla, Nikhil ; Jolly, Pawan ; Bowen, Chris R. ; Taylor, John T. ; Bowen, Jenna L. ; Allender, Chris J. ; Estrela, Pedro. / Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma. In: Analytical Chemistry. 2016 ; Vol. 88, No. 23. pp. 11486-11490.
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Tamboli, VK, Bhalla, N, Jolly, P, Bowen, CR, Taylor, JT, Bowen, JL, Allender, CJ & Estrela, P 2016, 'Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma', Analytical Chemistry, vol. 88, no. 23, pp. 11486-11490. https://doi.org/10.1021/acs.analchem.6b02619

Hybrid synthetic receptors on MOSFET devices for detection of prostate specific antigen in human plasma. / Tamboli, Vibha K.; Bhalla, Nikhil; Jolly, Pawan; Bowen, Chris R.; Taylor, John T.; Bowen, Jenna L.; Allender, Chris J.; Estrela, Pedro.

In: Analytical Chemistry, Vol. 88, No. 23, 06.12.2016, p. 11486-11490.

Research output: Contribution to journalArticle

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