Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films

TIT Okpalugo, H Murphy, AA Ogwu, GA Abbas, SC Ray, PD Maguire, JAD McLaughlin, RW McCullough

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40% N-dissociation and N-atomic fluxes of similar to 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films,semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I-D/I-G ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it evenfurther. In this study, lower Raman I-D/I-G ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering. (c) 2006 Wiley Periodicals. Inc.
LanguageEnglish
Pages222-229
JournalJournal of Biomedical Materials Research Part B: Applied Biomaterials
Volume78B
Issue number2
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Diamond
Carbon films
Diamonds
Carbon
Thin films
Nitrogen
Endothelial cells
Doping (additives)
Contact angle
Semiconducting films
Ions
Atoms
Diamond like carbon films
Plasma sources
Biocompatible Materials
Biocompatibility
Tissue engineering
Biomaterials
Photoluminescence
Adhesion

Keywords

  • biocompatibility/hard tissue
  • carbon coatings
  • cell-material interactions
  • endothelial cells
  • surface modification/characterization

Cite this

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title = "Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films",
abstract = "This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40{\%} N-dissociation and N-atomic fluxes of similar to 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films,semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I-D/I-G ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it evenfurther. In this study, lower Raman I-D/I-G ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering. (c) 2006 Wiley Periodicals. Inc.",
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Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films. / Okpalugo, TIT; Murphy, H; Ogwu, AA; Abbas, GA; Ray, SC; Maguire, PD; McLaughlin, JAD; McCullough, RW.

In: Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 78B, No. 2, 08.2006, p. 222-229.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films

AU - Okpalugo, TIT

AU - Murphy, H

AU - Ogwu, AA

AU - Abbas, GA

AU - Ray, SC

AU - Maguire, PD

AU - McLaughlin, JAD

AU - McCullough, RW

PY - 2006/8

Y1 - 2006/8

N2 - This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40% N-dissociation and N-atomic fluxes of similar to 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films,semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I-D/I-G ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it evenfurther. In this study, lower Raman I-D/I-G ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering. (c) 2006 Wiley Periodicals. Inc.

AB - This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40% N-dissociation and N-atomic fluxes of similar to 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films,semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I-D/I-G ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it evenfurther. In this study, lower Raman I-D/I-G ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering. (c) 2006 Wiley Periodicals. Inc.

KW - biocompatibility/hard tissue

KW - carbon coatings

KW - cell-material interactions

KW - endothelial cells

KW - surface modification/characterization

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DO - 10.1002/jbm.b.30459

M3 - Article

VL - 78B

SP - 222

EP - 229

JO - Journal of Biomedical Materials Research Part B: Applied Biomaterials

T2 - Journal of Biomedical Materials Research Part B: Applied Biomaterials

JF - Journal of Biomedical Materials Research Part B: Applied Biomaterials

SN - 1552-4973

IS - 2

ER -