Human immunodeficiency virus type 1 gag-specific mucosal immunity after oral immunization with papillomavirus pseudoviruses encoding gag

HT Zhang, R Fayad, XL Wang, Daniel Quinn, L Qiao

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The pseudoviruses are synthetic, nonreplicating viruses, yet they can produce antigens for a long time in the immune system. Here we show that oral immunization of mice by the use of papillomavirus pseudoviruses encoding Gag generated mucosal and systemic Gag-specific cytotoxic T lymphocytes that effectively lysed Gag-expressing target cells. Furthermore, the pseudoviruses generated Gag-specific gamma interferon-producing T cells and serum immunoglobulin G (IgG) and mucosal IgA. In contrast, oral immunization with plasmid DNA encoding HIV-1 Gag did not induce specific immune responses. Importantly, oral immunization with the pseudoviruses induced Gag-specific memory cytotoxic T lymphocytes and protected mice against a rectal mucosal challenge with a recombinant vaccinia virus expressing HIV-1 Gag. Thus, papillomavirus pseudoviruses encoding Gag are a promising mucosal vaccine against AIDS.
    LanguageEnglish
    Pages10249-10257
    JournalJournal of Virology
    Volume78
    Issue number19
    DOIs
    Publication statusPublished - Oct 2004

    Fingerprint

    Mucosal Immunity
    HIV-1
    Immunization
    Cytotoxic T-Lymphocytes
    HIV
    AIDS Vaccines
    Vaccinia virus
    Immunoglobulin A
    Interferon-gamma
    Immune System
    Plasmids
    Vaccines
    Immunoglobulin G
    Viruses
    T-Lymphocytes
    Antigens
    DNA
    Serum

    Cite this

    Zhang, HT ; Fayad, R ; Wang, XL ; Quinn, Daniel ; Qiao, L. / Human immunodeficiency virus type 1 gag-specific mucosal immunity after oral immunization with papillomavirus pseudoviruses encoding gag. In: Journal of Virology. 2004 ; Vol. 78, No. 19. pp. 10249-10257.
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    abstract = "Mucosal surfaces are the primary portals for human immunodeficiency virus (HIV) transmission. Because systemic immunization, in general, does not induce effective mucosal immune responses, a mucosal HIV vaccine is urgently needed. For this study, we developed papillomavirus pseudoviruses that express HIV-1 Gag. The pseudoviruses are synthetic, nonreplicating viruses, yet they can produce antigens for a long time in the immune system. Here we show that oral immunization of mice by the use of papillomavirus pseudoviruses encoding Gag generated mucosal and systemic Gag-specific cytotoxic T lymphocytes that effectively lysed Gag-expressing target cells. Furthermore, the pseudoviruses generated Gag-specific gamma interferon-producing T cells and serum immunoglobulin G (IgG) and mucosal IgA. In contrast, oral immunization with plasmid DNA encoding HIV-1 Gag did not induce specific immune responses. Importantly, oral immunization with the pseudoviruses induced Gag-specific memory cytotoxic T lymphocytes and protected mice against a rectal mucosal challenge with a recombinant vaccinia virus expressing HIV-1 Gag. Thus, papillomavirus pseudoviruses encoding Gag are a promising mucosal vaccine against AIDS.",
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    Human immunodeficiency virus type 1 gag-specific mucosal immunity after oral immunization with papillomavirus pseudoviruses encoding gag. / Zhang, HT; Fayad, R; Wang, XL; Quinn, Daniel; Qiao, L.

    In: Journal of Virology, Vol. 78, No. 19, 10.2004, p. 10249-10257.

    Research output: Contribution to journalArticle

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