Human Fetal Osteoblast Response on Poly(Methyl Methacrylate)/Polystyrene Demixed Thin Film Blends: Surface Chemistry Vs Topography Effects

Brian Meenan, Raechelle D'Sa, Peter Dickinson, Fiona Mccabe

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Recent advances in materials sciences have allowed for the development and fabrication of biomaterials that are capable of providing requisite cues to instigate cells to respond in a predictable fashion. We have developed a series of poly(methyl methacrylate)/polystyrene (PMMA/PS) polymer demixed thin films with nanotopographies ranging from nanoislands to nanopits to study the response of human fetal osteoblast cells (hFOBs). When PMMA was in excess in the blend composition, a nanoisland topography dominated, whereas a nanopit topography dominated when PS was in excess. PMMA was found to segregate to the top of the nanoisland morphology with PS preferring the substrate interface. To further ascertain the effects of surface chemistry vs topography, we plasma treated the polymer demixed films using an atmospheric pressure dielectric barrier discharge reactor to alter the surface chemistry. Our results have shown that hFOBs did not have an increased short-term cellular response on pristine polymer demixed surfaces. However, increasing the hydrophilicty/wettability of the surfaces by oxygen functionalization causes an increase in the cellular response. These results indicate that topography alone is not sufficient to induce a positive cellular response, but the underlying surface chemistry is also important in regulating cell function.
Original languageEnglish
Pages (from-to)14920-14931
Number of pages12
JournalACS Applied Materials & Interfaces
Volume8
Early online date29 Dec 2015
DOIs
Publication statusPublished - 22 Jun 2016

Keywords

  • surface topography
  • surface chemistry
  • polymer demixing
  • cellular response
  • human fetal osteoblasts
  • plasma surface modification

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