Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

J. Michael Conlon; Agnes Sonnevend; Carlos Davidson; Anni Demandt; Thierry Jouenne

doi:10.1016/j.dci.2004.05.003

Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

J. Michael Conlon
, Agnes Sonnevend
, Carlos Davidson
, Anni Demandt
, Thierry Jouenne

School of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC ₅₀<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH₂) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

Original language	English
Pages (from-to)	83-90
Number of pages	8
Journal	Developmental and Comparative Immunology
Volume	29
Issue number	1
DOIs	https://doi.org/10.1016/j.dci.2004.05.003
Publication status	Published (in print/issue) - 2005

Funding

This work was supported by an Interdisciplinary Grant (03/12-8-03-01) and a Faculty Support Grant (NP/04/02) from the United Arab Emirates University. The authors thank Lowell Diller and Laura Burkholder, Department of Wildlife, Humboldt State University for help in collection of specimens.

Funders
United Arab Emirates University

Keywords

Antimicrobial peptide
Brevinin-1
Frog skin
HC, concentration producing 50% hemolysis
MIC, minimum inhibitory concentration
Phylogeny
Ranatuerin-2
Temporin

Access to Document

10.1016/j.dci.2004.05.003

Cite this

@article{26ec11bf36a845c4b98ef9f0bed705dd,

title = "Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora",

abstract = "Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50\% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.",

keywords = "Antimicrobial peptide, Brevinin-1, Frog skin, HC, concentration producing 50\% hemolysis, MIC, minimum inhibitory concentration, Phylogeny, Ranatuerin-2, Temporin",

author = "Conlon, \{J. Michael\} and Agnes Sonnevend and Carlos Davidson and Anni Demandt and Thierry Jouenne",

year = "2005",

doi = "10.1016/j.dci.2004.05.003",

language = "English",

volume = "29",

pages = "83--90",

journal = "Developmental and Comparative Immunology",

issn = "0145-305X",

publisher = "Elsevier Ltd",

number = "1",

}

TY - JOUR

T1 - Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

AU - Conlon, J. Michael

AU - Sonnevend, Agnes

AU - Davidson, Carlos

AU - Demandt, Anni

AU - Jouenne, Thierry

PY - 2005

Y1 - 2005

N2 - Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

AB - Antimicrobial peptides in the skin secretions of anurans constitute a component of the innate immunity that protects the organism against invading pathogens. Four peptides with antimicrobial activity were isolated in high yield from norepinephrine-stimulated skin secretions of the Northern red-legged frog Rana aurora aurora and their primary structures determined. Ranatuerin-2AUa (GILSSFKGVAKGVAKNLAGKLLDELKCKITGC) showed potent growth-inhibitory activity against a range of Gram-positive and Gram-negative bacteria (minimum inhibitory concentrations <20 μM) but low hemolytic activity against human erythrocytes (50% hemolysis at 290 μM). Brevinin-1AUa (FLPILAGLAAKLVPKVFCSITKKC) and brevinin-1AUb (FLPILAGLAANILPKVFCSITKKC) also showed potent antimicrobial activity but were strongly hemolytic (HC 50<10 μM). Temporin-1AUa (FLPIIGQLLSGLL.NH2) atypically lacked a basic amino acid residue and showed very weak antimicrobial and hemolytic activity. Its biological function remains to be established. The primary structures of the antimicrobial peptides are consistent with a close phylogenetic relationship between R. aurora, Rana boylii and Rana luteiventris.

KW - Antimicrobial peptide

KW - Brevinin-1

KW - Frog skin

KW - HC, concentration producing 50% hemolysis

KW - MIC, minimum inhibitory concentration

KW - Phylogeny

KW - Ranatuerin-2

KW - Temporin

UR - https://www.scopus.com/pages/publications/4344710178

U2 - 10.1016/j.dci.2004.05.003

DO - 10.1016/j.dci.2004.05.003

M3 - Article

C2 - 15325526

AN - SCOPUS:4344710178

SN - 0145-305X

VL - 29

SP - 83

EP - 90

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

IS - 1

ER -

Host-defense peptides isolated from the skin secretions of the Northern red-legged frog Rana aurora aurora

Abstract

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this