Host defense peptides from Lithobates forreri, Hylarana luctuosa, and Hylarana signata (Ranidae): Phylogenetic relationships inferred from primary structures of ranatuerin-2 and brevinin-2 peptides

J. Michael Conlon, Jolanta Kolodziejek, Milena Mechkarska, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, Hubert Vaudry, Per F. Nielsen, Norbert Nowotny, Jay D. King

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The primary structures of host-defense peptides present in frog skin secretions constitute useful molecular markers for establishing taxonomic classifications and investigating phylogenetic relationships between species within a particular genus. Peptidomic analysis has led to the characterization of multiple host-defense peptides in norepinephrine-stimulated skin secretions of three species of frogs from the family Ranidae: Lithobates forreri (Boulenger, 1883), Hylarana luctuosa (Peters, 1871), and Hylarana signata (Günther, 1872). The L. forreri secretions contain ranatuerin-2 (2 peptides), brevinin-1 (4 peptides), and temporin (1 peptide). The H. luctuosa secretions contain brevinin-2 (4 peptides), esculentin-1 (1 peptide), esculentin-2 (1 peptide), palustrin-2 (2 peptides), and temporin (2 peptides). The H. signata secretions contain brevinin-2 (4 peptides), brevinin-1 (5 peptides), palustrin-2 (1 peptide), and temporin (2 peptides). Cladistic analysis based upon the primary structures of 44 ranatuerin-2 peptides from 20 Lithobates species indicates a close phylogenetic relationship between L. forreri, Lithobates onca, and Lithobates yavapaiensis. A similar cladistic analysis based upon the primary structures of 27 brevinin-2 peptides from 8 Hylarana species provides support for a close phylogenetic relationship between H. signata and Hylarana picturata, while showing that the species are not conspecific, with H. luctuosa more distantly related.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume9
Issue number1
DOIs
Publication statusPublished (in print/issue) - Mar 2014

Bibliographical note

Funding Information:
This work was supported by a Faculty Support Grant and a NRF/University grant from U.A.E. University and by the Terry Fox Fund for Cancer Research . The authors thank Teya King for help in collecting the frog species and Laurey Steinke and Michele Fontaine (University of Nebraska Medical Center, Omaha, NE) for amino acid composition analysis.

Keywords

  • Antimicrobial peptide Lithobates
  • Brevinin-2
  • Hylarana
  • Phylogeny
  • Ranatuerin-2

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