Host defense against viral infection involves interferon mediated down-regulation of sterol biosynthesis

Mathieu Blanc, Wayne Hsieh, Kevin A Robertson, Steven Watterson, Guanghou Shui, Paul Lacaze, Mizanur Khondoker, Paul Dickinson, Garwin Sing, Sara Rodriguez-Martin, Peter Phelan, Thorsten Forster, Birgit Strobl, Matthias Muller, Rudolph Riemersma, Timothy Osborne, Markus R Wenk, Ana Angulo, Peter Ghazal

    Research output: Contribution to journalArticle

    115 Citations (Scopus)

    Abstract

    Little is known about the protective role of inflammatory processes in modulating lipid metabolism in infection. Here we report an intimate link between the innate immune response to infection and regulation of the sterol metabolic network characterized by down-regulation of sterol biosynthesis by an interferon regulatory loop mechanism. In time-series experiments profiling genome-wide lipid-associated gene expression of macrophages, we show a selective and coordinated negative regulation of the complete sterol pathway upon viral infection or cytokine treatment with IFNc or b but not TNF, IL1b, or IL6. Quantitative analysis at the protein level of selected sterol metabolic enzymes upon infection shows a similar level of suppression. Experimental testing of sterol metabolite levels using lipidomic-based measurements shows a reduction in metabolic output. On the basis of pharmacologic and RNAi inhibition of the sterol pathway we show augmented protection against viral infection, and in combination with metabolite rescue experiments, we identify the requirement of the mevalonate-isoprenoid branch of the sterol metabolic network in the protective response upon statin or IFNb treatment. Conditioned media experiments from infected cells support an involvement of secreted type 1 interferon(s) to be sufficient for reducing the sterol pathway upon infection. Moreover, we show that infection of primary macrophages containing a genetic knockout of the major type I interferon, IFNb, leads to only a partial suppression of the sterol pathway, while genetic knockout of the receptor for all type I interferon family members, ifnar1, or associated signaling component, tyk2, completely abolishes the reduction of the sterol biosynthetic activity upon infection. Levels of the proteolytically cleaved nuclear forms of SREBP2, a key transcriptional regulator of sterol biosynthesis, are reduced upon infection and IFNb treatment at both the protein and de novo transcription level. The reduction in srebf2 gene transcription upon infection and IFN treatment is also found to be strictly dependent on ifnar1. Altogether these results show that type 1 IFN signaling is both necessary and sufficient for reducing the sterol metabolic network activity upon infection, thereby linking the regulation of the sterol pathway with interferon anti-viral defense responses. These findings bring a new link between sterol metabolism and interferon antiviral response and support the idea of using host metabolic modifiers of innate immunity as a potential antiviral strategy.
    LanguageEnglish
    Pagese1000598
    JournalPLoS Biology
    Volume9
    Issue number3
    DOIs
    Publication statusPublished - 8 Mar 2011

    Fingerprint

    Sterols
    Virus Diseases
    Interferons
    Down-Regulation
    Infection
    Metabolic Networks and Pathways
    Interferon Type I
    Innate Immunity
    Antiviral Agents
    Macrophages
    Interferon alpha-beta Receptor
    Hydroxymethylglutaryl-CoA Reductase Inhibitors
    Mevalonic Acid
    Terpenes
    Conditioned Culture Medium
    RNA Interference
    Lipid Metabolism
    Interleukin-6
    Proteins

    Keywords

    • interferon
    • macrophage
    • cholesterol
    • sterol
    • pathway
    • systems biology

    Cite this

    Blanc, Mathieu ; Hsieh, Wayne ; Robertson, Kevin A ; Watterson, Steven ; Shui, Guanghou ; Lacaze, Paul ; Khondoker, Mizanur ; Dickinson, Paul ; Sing, Garwin ; Rodriguez-Martin, Sara ; Phelan, Peter ; Forster, Thorsten ; Strobl, Birgit ; Muller, Matthias ; Riemersma, Rudolph ; Osborne, Timothy ; Wenk, Markus R ; Angulo, Ana ; Ghazal, Peter. / Host defense against viral infection involves interferon mediated down-regulation of sterol biosynthesis. In: PLoS Biology. 2011 ; Vol. 9, No. 3. pp. e1000598.
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    Blanc, M, Hsieh, W, Robertson, KA, Watterson, S, Shui, G, Lacaze, P, Khondoker, M, Dickinson, P, Sing, G, Rodriguez-Martin, S, Phelan, P, Forster, T, Strobl, B, Muller, M, Riemersma, R, Osborne, T, Wenk, MR, Angulo, A & Ghazal, P 2011, 'Host defense against viral infection involves interferon mediated down-regulation of sterol biosynthesis', PLoS Biology, vol. 9, no. 3, pp. e1000598. https://doi.org/10.1371/journal.pbio.1000598

    Host defense against viral infection involves interferon mediated down-regulation of sterol biosynthesis. / Blanc, Mathieu; Hsieh, Wayne; Robertson, Kevin A; Watterson, Steven; Shui, Guanghou; Lacaze, Paul; Khondoker, Mizanur; Dickinson, Paul; Sing, Garwin; Rodriguez-Martin, Sara; Phelan, Peter; Forster, Thorsten; Strobl, Birgit; Muller, Matthias; Riemersma, Rudolph; Osborne, Timothy; Wenk, Markus R; Angulo, Ana; Ghazal, Peter.

    In: PLoS Biology, Vol. 9, No. 3, 08.03.2011, p. e1000598.

    Research output: Contribution to journalArticle

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    AU - Hsieh, Wayne

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    AU - Strobl, Birgit

    AU - Muller, Matthias

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    AB - Little is known about the protective role of inflammatory processes in modulating lipid metabolism in infection. Here we report an intimate link between the innate immune response to infection and regulation of the sterol metabolic network characterized by down-regulation of sterol biosynthesis by an interferon regulatory loop mechanism. In time-series experiments profiling genome-wide lipid-associated gene expression of macrophages, we show a selective and coordinated negative regulation of the complete sterol pathway upon viral infection or cytokine treatment with IFNc or b but not TNF, IL1b, or IL6. Quantitative analysis at the protein level of selected sterol metabolic enzymes upon infection shows a similar level of suppression. Experimental testing of sterol metabolite levels using lipidomic-based measurements shows a reduction in metabolic output. On the basis of pharmacologic and RNAi inhibition of the sterol pathway we show augmented protection against viral infection, and in combination with metabolite rescue experiments, we identify the requirement of the mevalonate-isoprenoid branch of the sterol metabolic network in the protective response upon statin or IFNb treatment. Conditioned media experiments from infected cells support an involvement of secreted type 1 interferon(s) to be sufficient for reducing the sterol pathway upon infection. Moreover, we show that infection of primary macrophages containing a genetic knockout of the major type I interferon, IFNb, leads to only a partial suppression of the sterol pathway, while genetic knockout of the receptor for all type I interferon family members, ifnar1, or associated signaling component, tyk2, completely abolishes the reduction of the sterol biosynthetic activity upon infection. Levels of the proteolytically cleaved nuclear forms of SREBP2, a key transcriptional regulator of sterol biosynthesis, are reduced upon infection and IFNb treatment at both the protein and de novo transcription level. The reduction in srebf2 gene transcription upon infection and IFN treatment is also found to be strictly dependent on ifnar1. Altogether these results show that type 1 IFN signaling is both necessary and sufficient for reducing the sterol metabolic network activity upon infection, thereby linking the regulation of the sterol pathway with interferon anti-viral defense responses. These findings bring a new link between sterol metabolism and interferon antiviral response and support the idea of using host metabolic modifiers of innate immunity as a potential antiviral strategy.

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