HorB (HP0127) is a Gastric Epithelial Cell Adhesin

William J Snelling, Anthony Moran, Kieran Ryan, Paul Scully, Kieran McGourty, Jakki Cooney, Heidi Annuk, Paul O'Toole

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background:  The Helicobacter pylori protein HorB (encoded by HP0127) is a member of a paralogous family that includes the adhesins BabA, AlpA, AlpB, and HopZ, which contribute to adhesion to gastric epithelial cells. Of the verified H. pylori porins, the HorB sequence is most similar to that of HopE, but the function of HorB is unknown. The aim of our study was to investigate the role of HorB in H. pylori gastric epithelial cell adhesion.

Materials and methods:  We disrupted the horB gene in H. pylori and measured the adhesion to gastric epithelial cells (AGS cells). We then assessed the effect that HorB disruption had on lipopolysaccharide (LPS) O‐chain production and Lewis x and Lewis y antigen expression. A HorB mutant in the mouse‐adapted strain H. pylori SS1 was created by marker exchange and mouse stomach colonization was quantified. Using reverse transcription polymerase chain reaction, human gastric biopsy material from H. pylori‐infected patients was then examined for expression of the horB gene.

Results:  Disruption of the horB gene reduced H. pylori adhesion by more than twofold. Adhesion in the horB knockout strain was restored to wild‐type levels by re‐introduction of HorB into the chromosome. Disruption of HorB reduced production of LPS O‐chains and lowered the level of expression of Lewis x and Lewis y antigens. Insertional mutagenesis of the horB gene in H. pylori SS1 reduced mouse stomach colonization threefold. Finally, expression of the horB gene was detected in human gastric biopsy material from H. pylori‐infected patients.

Conclusions:  From these data we conclude that HorB has a role in H. pylori adhesion during infection.
LanguageEnglish
Article numberPMID: 17492999
Pages200
Number of pages209
JournalHelicobacter
Volume12
Issue number3
DOIs
Publication statusPublished - 4 May 2007

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Helicobacter pylori
Stomach
Epithelial Cells
Lipopolysaccharides
Hope
Genes
Biopsy
Gene Expression
Antigens
Porins
Insertional Mutagenesis
Cell Adhesion
Reverse Transcription
Chromosomes
Polymerase Chain Reaction
Infection

Cite this

Snelling, W. J., Moran, A., Ryan, K., Scully, P., McGourty, K., Cooney, J., ... O'Toole, P. (2007). HorB (HP0127) is a Gastric Epithelial Cell Adhesin. Helicobacter, 12(3), 200. [PMID: 17492999]. https://doi.org/10.1111/j.1523-5378.2007.00499.x
Snelling, William J ; Moran, Anthony ; Ryan, Kieran ; Scully, Paul ; McGourty, Kieran ; Cooney, Jakki ; Annuk, Heidi ; O'Toole, Paul. / HorB (HP0127) is a Gastric Epithelial Cell Adhesin. In: Helicobacter. 2007 ; Vol. 12, No. 3. pp. 200.
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abstract = "Background:  The Helicobacter pylori protein HorB (encoded by HP0127) is a member of a paralogous family that includes the adhesins BabA, AlpA, AlpB, and HopZ, which contribute to adhesion to gastric epithelial cells. Of the verified H. pylori porins, the HorB sequence is most similar to that of HopE, but the function of HorB is unknown. The aim of our study was to investigate the role of HorB in H. pylori gastric epithelial cell adhesion.Materials and methods:  We disrupted the horB gene in H. pylori and measured the adhesion to gastric epithelial cells (AGS cells). We then assessed the effect that HorB disruption had on lipopolysaccharide (LPS) O‐chain production and Lewis x and Lewis y antigen expression. A HorB mutant in the mouse‐adapted strain H. pylori SS1 was created by marker exchange and mouse stomach colonization was quantified. Using reverse transcription polymerase chain reaction, human gastric biopsy material from H. pylori‐infected patients was then examined for expression of the horB gene.Results:  Disruption of the horB gene reduced H. pylori adhesion by more than twofold. Adhesion in the horB knockout strain was restored to wild‐type levels by re‐introduction of HorB into the chromosome. Disruption of HorB reduced production of LPS O‐chains and lowered the level of expression of Lewis x and Lewis y antigens. Insertional mutagenesis of the horB gene in H. pylori SS1 reduced mouse stomach colonization threefold. Finally, expression of the horB gene was detected in human gastric biopsy material from H. pylori‐infected patients.Conclusions:  From these data we conclude that HorB has a role in H. pylori adhesion during infection.",
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Snelling, WJ, Moran, A, Ryan, K, Scully, P, McGourty, K, Cooney, J, Annuk, H & O'Toole, P 2007, 'HorB (HP0127) is a Gastric Epithelial Cell Adhesin', Helicobacter, vol. 12, no. 3, PMID: 17492999, pp. 200. https://doi.org/10.1111/j.1523-5378.2007.00499.x

HorB (HP0127) is a Gastric Epithelial Cell Adhesin. / Snelling, William J; Moran, Anthony ; Ryan, Kieran; Scully, Paul; McGourty, Kieran ; Cooney, Jakki ; Annuk, Heidi ; O'Toole, Paul.

In: Helicobacter, Vol. 12, No. 3, PMID: 17492999, 04.05.2007, p. 200.

Research output: Contribution to journalArticle

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T1 - HorB (HP0127) is a Gastric Epithelial Cell Adhesin

AU - Snelling, William J

AU - Moran, Anthony

AU - Ryan, Kieran

AU - Scully, Paul

AU - McGourty, Kieran

AU - Cooney, Jakki

AU - Annuk, Heidi

AU - O'Toole, Paul

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N2 - Background:  The Helicobacter pylori protein HorB (encoded by HP0127) is a member of a paralogous family that includes the adhesins BabA, AlpA, AlpB, and HopZ, which contribute to adhesion to gastric epithelial cells. Of the verified H. pylori porins, the HorB sequence is most similar to that of HopE, but the function of HorB is unknown. The aim of our study was to investigate the role of HorB in H. pylori gastric epithelial cell adhesion.Materials and methods:  We disrupted the horB gene in H. pylori and measured the adhesion to gastric epithelial cells (AGS cells). We then assessed the effect that HorB disruption had on lipopolysaccharide (LPS) O‐chain production and Lewis x and Lewis y antigen expression. A HorB mutant in the mouse‐adapted strain H. pylori SS1 was created by marker exchange and mouse stomach colonization was quantified. Using reverse transcription polymerase chain reaction, human gastric biopsy material from H. pylori‐infected patients was then examined for expression of the horB gene.Results:  Disruption of the horB gene reduced H. pylori adhesion by more than twofold. Adhesion in the horB knockout strain was restored to wild‐type levels by re‐introduction of HorB into the chromosome. Disruption of HorB reduced production of LPS O‐chains and lowered the level of expression of Lewis x and Lewis y antigens. Insertional mutagenesis of the horB gene in H. pylori SS1 reduced mouse stomach colonization threefold. Finally, expression of the horB gene was detected in human gastric biopsy material from H. pylori‐infected patients.Conclusions:  From these data we conclude that HorB has a role in H. pylori adhesion during infection.

AB - Background:  The Helicobacter pylori protein HorB (encoded by HP0127) is a member of a paralogous family that includes the adhesins BabA, AlpA, AlpB, and HopZ, which contribute to adhesion to gastric epithelial cells. Of the verified H. pylori porins, the HorB sequence is most similar to that of HopE, but the function of HorB is unknown. The aim of our study was to investigate the role of HorB in H. pylori gastric epithelial cell adhesion.Materials and methods:  We disrupted the horB gene in H. pylori and measured the adhesion to gastric epithelial cells (AGS cells). We then assessed the effect that HorB disruption had on lipopolysaccharide (LPS) O‐chain production and Lewis x and Lewis y antigen expression. A HorB mutant in the mouse‐adapted strain H. pylori SS1 was created by marker exchange and mouse stomach colonization was quantified. Using reverse transcription polymerase chain reaction, human gastric biopsy material from H. pylori‐infected patients was then examined for expression of the horB gene.Results:  Disruption of the horB gene reduced H. pylori adhesion by more than twofold. Adhesion in the horB knockout strain was restored to wild‐type levels by re‐introduction of HorB into the chromosome. Disruption of HorB reduced production of LPS O‐chains and lowered the level of expression of Lewis x and Lewis y antigens. Insertional mutagenesis of the horB gene in H. pylori SS1 reduced mouse stomach colonization threefold. Finally, expression of the horB gene was detected in human gastric biopsy material from H. pylori‐infected patients.Conclusions:  From these data we conclude that HorB has a role in H. pylori adhesion during infection.

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DO - 10.1111/j.1523-5378.2007.00499.x

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SN - 1083-4389

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Snelling WJ, Moran A, Ryan K, Scully P, McGourty K, Cooney J et al. HorB (HP0127) is a Gastric Epithelial Cell Adhesin. Helicobacter. 2007 May 4;12(3):200. PMID: 17492999. https://doi.org/10.1111/j.1523-5378.2007.00499.x