High-Sensitive Detection and Quantitative Analysis of Thyroid-Stimulating Hormone Using Gold-Nanoshell-Based Lateral Flow Immunoassay Device

Santosh Kumar Bikkarolla, Sara E McNamee, Paul Vance, James McLaughlin

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Abstract

Au nanoparticles (AuNPs) have been used as signal reporters in colorimetric lateral flow immunoassays (LFAs) for decades. However, it remains a major challenge to significantly improve the detection sensitivity of traditional LFAs due to the low brightness of AuNPs. As an alternative approach, we overcome this problem by utilizing 150 nm gold nanoshells (AuNSs) that were engineered by coating low-density silica nanoparticles with a thin layer of gold. AuNSs are dark green, have 14 times larger surface area, and are approximately 35 times brighter compared to AuNPs. In this study, we used detection of thyroid-stimulating hormone (TSH) in a proof-of-concept assay. The limit of detection (LOD) with AuNS-based LFA was 0.16 µIU/mL, which is 26 times more sensitive than the conventional colorimetric LFA that utilizes AuNP as a label. The dynamic range of the calibration curve was 0.16-9.5 µIU/mL, making it possible to diagnose both hyperthyroidism (<0.5 µIU/mL) and hypothyroidism (>5 µIU/mL) using AuNS-based LFA. Thus, the developed device has a strong potential for early screening and diagnosis of diseases related to the thyroid hormone.
Original languageEnglish
Article numbere182
Number of pages12
JournalBiosensors
Volume12
Issue number3
Early online date19 Mar 2022
DOIs
Publication statusE-pub ahead of print - 19 Mar 2022

Keywords

  • gold nanoparticles
  • thyroid-stimulating hormone
  • human plasma
  • lateral flow immunoassay
  • gold nanoshells
  • human serum
  • Gold
  • Immunoassay
  • Limit of Detection
  • Thyrotropin
  • Nanoshells
  • Metal Nanoparticles

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