HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

DT BURNS, M O'CALLAGHAN, Franklin Smyth, CJ AYLING

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    Abstract

    Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.
    LanguageEnglish
    Pages53-56
    JournalFresenius' Journal of Analytical Chemistry
    Volume340
    Issue number1
    Publication statusPublished - 1991

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    Cloxacillin
    High performance liquid chromatography
    Ampicillin
    Veterinary medicine
    Degradation
    Penicillins
    Recovery

    Cite this

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    title = "HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION",
    abstract = "Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8{\%} for ampicillin with coefficient of variation of 1.0 and 103.4{\%} for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.",
    author = "DT BURNS and M O'CALLAGHAN and Franklin Smyth and CJ AYLING",
    year = "1991",
    language = "English",
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    AU - O'CALLAGHAN, M

    AU - Smyth, Franklin

    AU - AYLING, CJ

    PY - 1991

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    AB - Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.

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