Abstract
| Language | English |
|---|---|
| Pages | 53-56 |
| Journal | Fresenius' Journal of Analytical Chemistry |
| Volume | 340 |
| Issue number | 1 |
| Publication status | Published - 1991 |
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HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION. / BURNS, DT; O'CALLAGHAN, M; Smyth, Franklin; AYLING, CJ.
In: Fresenius' Journal of Analytical Chemistry, Vol. 340, No. 1, 1991, p. 53-56.Research output: Contribution to journal › Article
TY - JOUR
T1 - HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION
AU - BURNS, DT
AU - O'CALLAGHAN, M
AU - Smyth, Franklin
AU - AYLING, CJ
PY - 1991
Y1 - 1991
N2 - Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.
AB - Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.
M3 - Article
VL - 340
SP - 53
EP - 56
JO - Fresenius' Journal of Analytical Chemistry
T2 - Fresenius' Journal of Analytical Chemistry
JF - Fresenius' Journal of Analytical Chemistry
SN - 0937-0633
IS - 1
ER -