HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

DT BURNS, M O'CALLAGHAN, Franklin Smyth, CJ AYLING

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.
    Original languageEnglish
    Pages (from-to)53-56
    JournalFresenius' Journal of Analytical Chemistry
    Volume340
    Issue number1
    Publication statusPublished - 1991

    Fingerprint Dive into the research topics of 'HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION'. Together they form a unique fingerprint.

  • Cite this