HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

DT BURNS; M O'CALLAGHAN; Franklin Smyth; CJ AYLING

HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

DT BURNS, M O'CALLAGHAN, Franklin Smyth, CJ AYLING

Research output: Contribution to journal › Article › peer-review

Abstract

Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.

Original language	English
Pages (from-to)	53-56
Journal	Fresenius' Journal of Analytical Chemistry
Volume	340
Issue number	1
Publication status	Published - 1991

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@article{dacd4698bfb14083911d05a97e83357a,

title = "HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION",

abstract = "Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.",

author = "DT BURNS and M O'CALLAGHAN and Franklin Smyth and CJ AYLING",

year = "1991",

language = "English",

volume = "340",

pages = "53--56",

journal = "Fresenius' Journal of Analytical Chemistry",

issn = "0937-0633",

publisher = "Springer",

number = "1",

}

TY - JOUR

T1 - HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF AMPICILLIN AND CLOXACILLIN AND ITS APPLICATION TO AN INTRAMAMMARY VETERINARY PREPARATION

AU - BURNS, DT

AU - O'CALLAGHAN, M

AU - Smyth, Franklin

AU - AYLING, CJ

PY - 1991

Y1 - 1991

N2 - Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.

AB - Reverse phase high-performance liquid chromatography with ultra-violet detection has been used for the simultaneous identification and determination of ampicillin, cloxacillin and some of their degradation products following their extraction from an intramammary preparation used in veterinary medicine. Extraction of the intramammary base into petroleum ether (40-60-degrees-C) and partition of the penicillins into the mobile phase gave a mean recovery of 100.8% for ampicillin with coefficient of variation of 1.0 and 103.4% for cloxacillin with coefficient of variation of 1.2 (n = 6). Using optimised HPLC conditions ampicillin eluted in approximately 2 min and cloxacillin in approximately 8 min. The overall method was found to be stability indicating, since cloxacillin and ampicillin eluted independently of their degradation products.

M3 - Article

VL - 340

SP - 53

EP - 56

JO - Fresenius' Journal of Analytical Chemistry

JF - Fresenius' Journal of Analytical Chemistry

SN - 0937-0633

IS - 1

ER -