H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS

K SVENSSON, Colum Walsh, R FUNDELE, R OHLSSON

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

It has been proposed that either the Igf-2 gene or the H19 gene - but not both - can be expressed from a given chromosome. Igf-2 is known to be biallelically expressed in the choroid plexus and leptomeninges, however, raising the question of whether H19 is down-regulated or absent there. We found by in situ hybridization that H19 is indeed expressed in the choroid plexus and leptomeninges of the developing mouse foetus. Comparison with the expression pattern of Igf-2 showed that the genes are coexpressed in all areas, with the exception of the choroid plexus epithelium. To evaluate whether H19 is also biallelically expressed in these tissues, we microdissected embryos from interspecific crosses and performed RNAse protection analysis on the isolated RNA. This revealed that H19 maintains its imprint in the choroid plexus/leptomeninges, being transcribed from the maternal allele at a level comparable to that in normal liver. We discuss the significance of these results for current models of Igf-2 and H19 imprinting.
LanguageEnglish
Pages31-37
JournalMechanisms of Development
Volume51
Issue number1
DOIs
Publication statusPublished - May 1995

Fingerprint

Choroid Plexus
Fetus
Genes
In Situ Hybridization
Embryonic Structures
Epithelium
Chromosomes
Alleles
Mothers
RNA
Liver

Cite this

SVENSSON, K ; Walsh, Colum ; FUNDELE, R ; OHLSSON, R. / H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS. In: Mechanisms of Development. 1995 ; Vol. 51, No. 1. pp. 31-37.
@article{e44b304043be46d281117028d89e210e,
title = "H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS",
abstract = "It has been proposed that either the Igf-2 gene or the H19 gene - but not both - can be expressed from a given chromosome. Igf-2 is known to be biallelically expressed in the choroid plexus and leptomeninges, however, raising the question of whether H19 is down-regulated or absent there. We found by in situ hybridization that H19 is indeed expressed in the choroid plexus and leptomeninges of the developing mouse foetus. Comparison with the expression pattern of Igf-2 showed that the genes are coexpressed in all areas, with the exception of the choroid plexus epithelium. To evaluate whether H19 is also biallelically expressed in these tissues, we microdissected embryos from interspecific crosses and performed RNAse protection analysis on the isolated RNA. This revealed that H19 maintains its imprint in the choroid plexus/leptomeninges, being transcribed from the maternal allele at a level comparable to that in normal liver. We discuss the significance of these results for current models of Igf-2 and H19 imprinting.",
author = "K SVENSSON and Colum Walsh and R FUNDELE and R OHLSSON",
year = "1995",
month = "5",
doi = "10.1016/0925-4773(94)00345-N",
language = "English",
volume = "51",
pages = "31--37",
journal = "Mechanisms of Development",
issn = "0925-4773",
publisher = "Elsevier",
number = "1",

}

H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS. / SVENSSON, K; Walsh, Colum; FUNDELE, R; OHLSSON, R.

In: Mechanisms of Development, Vol. 51, No. 1, 05.1995, p. 31-37.

Research output: Contribution to journalArticle

TY - JOUR

T1 - H19 IS IMPRINTED IN THE CHOROID-PLEXUS AND LEPTOMENINGES OF THE MOUSE FETUS

AU - SVENSSON, K

AU - Walsh, Colum

AU - FUNDELE, R

AU - OHLSSON, R

PY - 1995/5

Y1 - 1995/5

N2 - It has been proposed that either the Igf-2 gene or the H19 gene - but not both - can be expressed from a given chromosome. Igf-2 is known to be biallelically expressed in the choroid plexus and leptomeninges, however, raising the question of whether H19 is down-regulated or absent there. We found by in situ hybridization that H19 is indeed expressed in the choroid plexus and leptomeninges of the developing mouse foetus. Comparison with the expression pattern of Igf-2 showed that the genes are coexpressed in all areas, with the exception of the choroid plexus epithelium. To evaluate whether H19 is also biallelically expressed in these tissues, we microdissected embryos from interspecific crosses and performed RNAse protection analysis on the isolated RNA. This revealed that H19 maintains its imprint in the choroid plexus/leptomeninges, being transcribed from the maternal allele at a level comparable to that in normal liver. We discuss the significance of these results for current models of Igf-2 and H19 imprinting.

AB - It has been proposed that either the Igf-2 gene or the H19 gene - but not both - can be expressed from a given chromosome. Igf-2 is known to be biallelically expressed in the choroid plexus and leptomeninges, however, raising the question of whether H19 is down-regulated or absent there. We found by in situ hybridization that H19 is indeed expressed in the choroid plexus and leptomeninges of the developing mouse foetus. Comparison with the expression pattern of Igf-2 showed that the genes are coexpressed in all areas, with the exception of the choroid plexus epithelium. To evaluate whether H19 is also biallelically expressed in these tissues, we microdissected embryos from interspecific crosses and performed RNAse protection analysis on the isolated RNA. This revealed that H19 maintains its imprint in the choroid plexus/leptomeninges, being transcribed from the maternal allele at a level comparable to that in normal liver. We discuss the significance of these results for current models of Igf-2 and H19 imprinting.

U2 - 10.1016/0925-4773(94)00345-N

DO - 10.1016/0925-4773(94)00345-N

M3 - Article

VL - 51

SP - 31

EP - 37

JO - Mechanisms of Development

T2 - Mechanisms of Development

JF - Mechanisms of Development

SN - 0925-4773

IS - 1

ER -