GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA

N BARRON; R MARCHANT; L MCHALE; AP MCHALE

doi:10.1007/BF00128612

GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA

N BARRON, R MARCHANT, L MCHALE, AP MCHALE

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.

Original language	English
Pages (from-to)	625-630
Journal	Biotechnology Letters
Volume	16
Issue number	6
DOIs	https://doi.org/10.1007/BF00128612
Publication status	Published (in print/issue) - Jun 1994

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title = "GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA",

abstract = "The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.",

author = "N BARRON and R MARCHANT and L MCHALE and AP MCHALE",

year = "1994",

month = jun,

doi = "10.1007/BF00128612",

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pages = "625--630",

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AU - BARRON, N

AU - MARCHANT, R

AU - MCHALE, L

AU - MCHALE, AP

PY - 1994/6

Y1 - 1994/6

N2 - The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.

AB - The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.

U2 - 10.1007/BF00128612

DO - 10.1007/BF00128612

M3 - Article

SN - 1573-6776

VL - 16

SP - 625

EP - 630

JO - Biotechnology Letters

JF - Biotechnology Letters

IS - 6

ER -

GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA

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