GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA

N BARRON, R MARCHANT, L MCHALE, AP MCHALE

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Abstract

The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.
LanguageEnglish
Pages625-630
JournalBiotechnology Letters
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 1994

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Kluyveromyces marxianus
cellobiose
beta-glucosidase
ethanol
ethanol production
organisms
culture filtrates
yeasts
sugars
cells

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title = "GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA",
abstract = "The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.",
author = "N BARRON and R MARCHANT and L MCHALE and AP MCHALE",
year = "1994",
month = "6",
doi = "10.1007/BF00128612",
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journal = "Biotechnology Letters",
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GROWTH OF A THERMOTOLERANT ETHANOL-PRODUCING STRAIN OF KLUYVEROMYCES-MARXIANUS ON CELLOBIOSE CONTAINING MEDIA. / BARRON, N; MARCHANT, R; MCHALE, L; MCHALE, AP.

In: Biotechnology Letters, Vol. 16, No. 6, 06.1994, p. 625-630.

Research output: Contribution to journalArticle

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AU - BARRON, N

AU - MARCHANT, R

AU - MCHALE, L

AU - MCHALE, AP

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AB - The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was shown to be capable of limited growth on cellobiose containing media at 45 degrees C. Growth, sugar utilization and ethanol production were shown to increase in the presence of exogenously added thermostable fungal beta-glucosidase. During active growth of the organism on cellobiose-containing media, beta-glucosidase activity was detected in cell lysate preparations with only minor amounts of activity present in the extracellular culture filtrate. The results suggest that limitations in ethanol production by this organism during growth on cellobiose containing media may be overcome by addition of exogenously added beta-glucosidase which results in increased substrate access to the biocatalytic unit.

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