Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction

A Erdem, M Muti, P Papakonstantinou, E Canavar, H Karadeniz, G Congur, S Sharma

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD % (n = 3) was calculated as 10.47% and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA
LanguageEnglish
Pages2129-2135
JournalANALYST
Volume137
DOIs
Publication statusPublished - 5 Mar 2012

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Mitomycin
Oxides
Monitoring
DNA
Sensors
Voltammetry
Electrochemical impedance spectroscopy
Pharmaceutical Preparations
Immobilized Nucleic Acids
Thymus
Electrochemical sensors
Electrodes
Guanine
Nucleic Acids
Charge transfer
Kinetics

Cite this

Erdem, A ; Muti, M ; Papakonstantinou, P ; Canavar, E ; Karadeniz, H ; Congur, G ; Sharma, S. / Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction. In: ANALYST. 2012 ; Vol. 137. pp. 2129-2135.
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abstract = "We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD {\%} (n = 3) was calculated as 10.47{\%} and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA",
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Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction. / Erdem, A; Muti, M; Papakonstantinou, P; Canavar, E; Karadeniz, H; Congur, G; Sharma, S.

In: ANALYST, Vol. 137, 05.03.2012, p. 2129-2135.

Research output: Contribution to journalArticle

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T1 - Graphene oxide integrated sensor for electrochemical monitoring ofmitomycin C–DNA interaction

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AU - Muti, M

AU - Papakonstantinou, P

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AU - Congur, G

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AB - We present a graphene oxide (GO) integrated disposable electrochemical sensor for the enhanced detection of nucleic acids and the sensitive monitoring of the surface-confined interactions between the anticancer drug mitomycin C (MC) and DNA. Interfacial interactions between immobilized calf thymus double-stranded (dsDNA) and anticancer drug MC were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. Based on three repetitive voltammetric measurements of 120 μg mL(-1) DNA immobilized on GO-modified electrodes, the RSD % (n = 3) was calculated as 10.47% and the detection limit (DL) for dsDNA was found to be 9.06 μg mL(-1). EIS studies revealed that the binding of the drug MC to dsDNA leads to a gradual decrease of its negative charge. As a consequence of this interaction, the negative redox species were allowed to approach the electrode, and thus increase the charge transfer kinetics. On the other hand, DPV studies exploited the decrease of the guanine signal due to drug binding as the basis for specifically probing the biointeraction process between MC and dsDNA

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