Granular gland transcriptomes in stimulated amphibian skin secretions

TB Chen, S Farragher, AJ Bjourson, DF Orr, P Rao, C Shaw

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Amphibian defensive skin secretions are complex, species-specific cocktails of biologically active molecules, including many uncharacterized peptides. The study of such secretions for novel peptide discovery is time-limited, as amphibians are in rapid global decline. While secretion proteome analysis is non-lethal, transcriptome analysis has until now required killing of specimens prior to skin dissection for cDNA library construction. Here we present the discovery that polyadenylated mRNAs encoding dermal granular gland peptides are present in defensive skin secretions, stabilized by endogenous nucleic acid-binding amphipathic peptides. Thus parallel secretory proteome and transcriptome analyses can be performed without killing the specimen in this model amphibian system - a finding that has important implications in conservation of biodiversity within this threatened vertebrate taxon and whose mechanistics may have broader implications in biomolecular science.
LanguageEnglish
Pages125-130
JournalBiochemical Journal
Volume371
Issue numberPart 1
Publication statusPublished - Apr 2003

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Amphibians
Transcriptome
Skin
Peptides
Gene Expression Profiling
Proteome
Biodiversity
Gene Library
Nucleic Acids
Vertebrates
Dissection
Messenger RNA

Cite this

Chen, TB., Farragher, S., Bjourson, AJ., Orr, DF., Rao, P., & Shaw, C. (2003). Granular gland transcriptomes in stimulated amphibian skin secretions. Biochemical Journal, 371(Part 1), 125-130.
Chen, TB ; Farragher, S ; Bjourson, AJ ; Orr, DF ; Rao, P ; Shaw, C. / Granular gland transcriptomes in stimulated amphibian skin secretions. In: Biochemical Journal. 2003 ; Vol. 371, No. Part 1. pp. 125-130.
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Chen, TB, Farragher, S, Bjourson, AJ, Orr, DF, Rao, P & Shaw, C 2003, 'Granular gland transcriptomes in stimulated amphibian skin secretions', Biochemical Journal, vol. 371, no. Part 1, pp. 125-130.

Granular gland transcriptomes in stimulated amphibian skin secretions. / Chen, TB; Farragher, S; Bjourson, AJ; Orr, DF; Rao, P; Shaw, C.

In: Biochemical Journal, Vol. 371, No. Part 1, 04.2003, p. 125-130.

Research output: Contribution to journalArticle

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Chen TB, Farragher S, Bjourson AJ, Orr DF, Rao P, Shaw C. Granular gland transcriptomes in stimulated amphibian skin secretions. Biochemical Journal. 2003 Apr;371(Part 1):125-130.