Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

Jacob Waitkus, Yu Chang, Li Liu, Srinivasu Valagerahally Puttaswamy, Taerin Chung, Adrian M. Molina Vargas, Stephen J. Dollery, Mitchell R. O'Connell, Haogang Cai, Gregory J. Tobin, Nikhil Bhalla, Ke Du

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A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.
Original languageEnglish
Article number2201261
Pages (from-to)1-9
Number of pages9
JournalAdvanced Materials Interfaces
Issue number1
Early online date27 Nov 2022
Publication statusPublished (in print/issue) - 5 Jan 2023

Bibliographical note

Funding Information:
This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM142763, Burroughs Wellcome Fund 1019955 (to K.D.), and R35GM133462 (to M.R.O.). The authors would like to thank Sunghwan Bae and Mary Nguyen for the schematic design.

Publisher Copyright:
© 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.


  • Research Article
  • Research Articles
  • CRISPR‐Cas13a
  • finite difference time domain
  • gold nanomushroom
  • gold nanoparticle
  • localized surface plasmon resonance (LSPR)
  • microfluidics
  • SARS‐CoV‐2
  • SARS-CoV-2
  • CRISPR-Cas13a


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