Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

Jacob Waitkus; Yu Chang; Li Liu; Srinivasu Valagerahally Puttaswamy; Taerin Chung; Adrian M. Molina Vargas; Stephen J. Dollery; Mitchell R. O'Connell; Haogang Cai; Gregory J. Tobin; Nikhil Bhalla; Ke Du

doi:10.1002/admi.202201261

Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

Jacob Waitkus, Yu Chang, Li Liu, Srinivasu Valagerahally Puttaswamy, Taerin Chung, Adrian M. Molina Vargas, Stephen J. Dollery, Mitchell R. O'Connell, Haogang Cai, Gregory J. Tobin, Nikhil Bhalla, Ke Du

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Abstract

A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.

Original language	English
Article number	2201261
Pages (from-to)	1-9
Number of pages	9
Journal	Advanced Materials Interfaces
Volume	10
Issue number	1
Early online date	27 Nov 2022
DOIs	https://doi.org/10.1002/admi.202201261
Publication status	Published (in print/issue) - 5 Jan 2023

Bibliographical note

Funding Information:
This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM142763, Burroughs Wellcome Fund 1019955 (to K.D.), and R35GM133462 (to M.R.O.). The authors would like to thank Sunghwan Bae and Mary Nguyen for the schematic design.

Publisher Copyright:
© 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.

Keywords

Research Article
Research Articles
CRISPR‐Cas13a
finite difference time domain
gold nanomushroom
gold nanoparticle
localized surface plasmon resonance (LSPR)
microfluidics
SARS‐CoV‐2
SARS-CoV-2
CRISPR-Cas13a

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Waitkus, J., Chang, Y., Liu, L., Puttaswamy, S. V., Chung, T., Molina Vargas, A. M., Dollery, S. J., O'Connell, M. R., Cai, H., Tobin, G. J., Bhalla, N., & Du, K. (2023). Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing. Advanced Materials Interfaces, 10(1), 1-9. Article 2201261. https://doi.org/10.1002/admi.202201261

@article{84ced3b36ae746d390d053acc29fdb9f,

title = "Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing",

abstract = "A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.",

keywords = "Research Article, Research Articles, CRISPR‐Cas13a, finite difference time domain, gold nanomushroom, gold nanoparticle, localized surface plasmon resonance (LSPR), microfluidics, SARS‐CoV‐2, SARS-CoV-2, CRISPR-Cas13a",

author = "Jacob Waitkus and Yu Chang and Li Liu and Puttaswamy, {Srinivasu Valagerahally} and Taerin Chung and {Molina Vargas}, {Adrian M.} and Dollery, {Stephen J.} and O'Connell, {Mitchell R.} and Haogang Cai and Tobin, {Gregory J.} and Nikhil Bhalla and Ke Du",

note = "Funding Information: This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM142763, Burroughs Wellcome Fund 1019955 (to K.D.), and R35GM133462 (to M.R.O.). The authors would like to thank Sunghwan Bae and Mary Nguyen for the schematic design. Publisher Copyright: {\textcopyright} 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.",

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doi = "10.1002/admi.202201261",

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Waitkus, J, Chang, Y, Liu, L, Puttaswamy, SV, Chung, T, Molina Vargas, AM, Dollery, SJ, O'Connell, MR, Cai, H, Tobin, GJ, Bhalla, N & Du, K 2023, 'Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing', Advanced Materials Interfaces, vol. 10, no. 1, 2201261, pp. 1-9. https://doi.org/10.1002/admi.202201261

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T1 - Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

AU - Waitkus, Jacob

AU - Chang, Yu

AU - Liu, Li

AU - Puttaswamy, Srinivasu Valagerahally

AU - Chung, Taerin

AU - Molina Vargas, Adrian M.

AU - Dollery, Stephen J.

AU - O'Connell, Mitchell R.

AU - Cai, Haogang

AU - Tobin, Gregory J.

AU - Bhalla, Nikhil

AU - Du, Ke

N1 - Funding Information: This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM142763, Burroughs Wellcome Fund 1019955 (to K.D.), and R35GM133462 (to M.R.O.). The authors would like to thank Sunghwan Bae and Mary Nguyen for the schematic design. Publisher Copyright: © 2022 The Authors. Advanced Materials Interfaces published by Wiley-VCH GmbH.

PY - 2023/1/5

Y1 - 2023/1/5

N2 - A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.

AB - A novel localized surface plasmon resonance (LSPR) system based on the coupling of gold nanomushrooms (AuNMs) and gold nanoparticles (AuNPs) is developed to enable a significant plasmonic resonant shift. The AuNP size, surface chemistry, and concentration are characterized to maximize the LSPR effect. A 31 nm redshift is achieved when the AuNMs are saturated by the AuNPs. This giant redshift also increases the full width of the spectrum and is explained by the 3D finite‐difference time‐domain (FDTD) calculation. In addition, this LSPR substrate is packaged in a microfluidic cell and integrated with a CRISPR‐Cas13a RNA detection assay for the detection of the SARS‐CoV‐2 RNA targets. Once activated by the target, the AuNPs are cleaved from linker probes and randomly deposited on the AuNM substrate, demonstrating a large redshift. The novel LSPR chip using AuNP as an indicator is simple, specific, isothermal, and label‐free; and thus, provides a new opportunity to achieve the next generation multiplexing and sensitive molecular diagnostic system.

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KW - Research Articles

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KW - gold nanomushroom

KW - gold nanoparticle

KW - localized surface plasmon resonance (LSPR)

KW - microfluidics

KW - SARS‐CoV‐2

KW - SARS-CoV-2

KW - CRISPR-Cas13a

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ER -

Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On‐Chip CRISPR‐Cas13a Sensing

Abstract

Bibliographical note

Keywords

UN SDGs

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