Gold-coated plant virus as computed tomography imaging contrast agent

Alaa A. A. Aljabali, Mazhar S. Al Zoubi, Khalid M. Al-Batanyeh, Ali Al-Radaideh, Mohammad A. Obeid, Abeer Al Sharabi, Walhan Alshaer, Bayan AbuFares, Tasnim Al-Zanatil, Murtaza M. Tambuwala, Naveed Akbar, David J. Evans

Research output: Contribution to journalArticle

Abstract

Biologically derived nanoparticles, such as proteins and viruses, provide unique platforms that allow the incorporation of novel properties due to their surface composition, their amenability for chemical and genetic engineering, monodispersity and biodegradability. Chemical modification of the virus surface, both the interior and the exterior, imparts new functionalities, that have potential applications in nanomedicine. In this study, we developed novel virus-based nanomaterials as a contrast agent for computed tomography (CT) imaging in vitro. The gold-coated cowpea mosaic virus (Au-CPMV) particles were generated by the electrostatic adsorption of positively charged electrolyte on the virus capsid with the subsequent incubation and reduction of anionic gold complexes to generate Au-CPMV particles. Au-CPMV particles as a CT contrast agent offer a fast scan time (less than 2 minutes) method, low cost, biocompatibility and a high-resolution image with ~ 150 Hounsfield Units (HU). The Au-CPMV surface was further modified allowing the incorporation of targeting molecules of specific cell types for imaging diseased cells.
LanguageEnglish
Pages1983-1993
Number of pages11
JournalBeilstein Journal of Nanotechnology
Volume10
DOIs
Publication statusPublished - 7 Oct 2019

Fingerprint

viruses
Viruses
Gold
Contrast Media
Tomography
tomography
gold
Imaging techniques
chemical engineering
Medical nanotechnology
biodegradability
Genetic engineering
Biodegradability
Chemical modification
biocompatibility
Chemical engineering
Image resolution
cells
Biocompatibility
Nanostructured materials

Keywords

  • biomedical imaging; computed tomography (CT); gold; nanotechnology; viruses; targeting
  • gold
  • viruses
  • targeting
  • computed tomography (CT)
  • nanotechnology
  • biomedical imaging

Cite this

Aljabali, A. A. A., Al Zoubi, M. S., Al-Batanyeh, K. M., Al-Radaideh, A., Obeid, M. A., Al Sharabi, A., ... Evans, D. J. (2019). Gold-coated plant virus as computed tomography imaging contrast agent. Beilstein Journal of Nanotechnology, 10, 1983-1993. https://doi.org/10.3762/bjnano.10.195
Aljabali, Alaa A. A. ; Al Zoubi, Mazhar S. ; Al-Batanyeh, Khalid M. ; Al-Radaideh, Ali ; Obeid, Mohammad A. ; Al Sharabi, Abeer ; Alshaer, Walhan ; AbuFares, Bayan ; Al-Zanatil, Tasnim ; Tambuwala, Murtaza M. ; Akbar, Naveed ; Evans, David J. / Gold-coated plant virus as computed tomography imaging contrast agent. In: Beilstein Journal of Nanotechnology. 2019 ; Vol. 10. pp. 1983-1993.
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Aljabali, AAA, Al Zoubi, MS, Al-Batanyeh, KM, Al-Radaideh, A, Obeid, MA, Al Sharabi, A, Alshaer, W, AbuFares, B, Al-Zanatil, T, Tambuwala, MM, Akbar, N & Evans, DJ 2019, 'Gold-coated plant virus as computed tomography imaging contrast agent', Beilstein Journal of Nanotechnology, vol. 10, pp. 1983-1993. https://doi.org/10.3762/bjnano.10.195

Gold-coated plant virus as computed tomography imaging contrast agent. / Aljabali, Alaa A. A.; Al Zoubi, Mazhar S.; Al-Batanyeh, Khalid M.; Al-Radaideh, Ali; Obeid, Mohammad A.; Al Sharabi, Abeer; Alshaer, Walhan; AbuFares, Bayan; Al-Zanatil, Tasnim; Tambuwala, Murtaza M.; Akbar, Naveed; Evans, David J.

In: Beilstein Journal of Nanotechnology, Vol. 10, 07.10.2019, p. 1983-1993.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gold-coated plant virus as computed tomography imaging contrast agent

AU - Aljabali, Alaa A. A.

AU - Al Zoubi, Mazhar S.

AU - Al-Batanyeh, Khalid M.

AU - Al-Radaideh, Ali

AU - Obeid, Mohammad A.

AU - Al Sharabi, Abeer

AU - Alshaer, Walhan

AU - AbuFares, Bayan

AU - Al-Zanatil, Tasnim

AU - Tambuwala, Murtaza M.

AU - Akbar, Naveed

AU - Evans, David J.

PY - 2019/10/7

Y1 - 2019/10/7

N2 - Biologically derived nanoparticles, such as proteins and viruses, provide unique platforms that allow the incorporation of novel properties due to their surface composition, their amenability for chemical and genetic engineering, monodispersity and biodegradability. Chemical modification of the virus surface, both the interior and the exterior, imparts new functionalities, that have potential applications in nanomedicine. In this study, we developed novel virus-based nanomaterials as a contrast agent for computed tomography (CT) imaging in vitro. The gold-coated cowpea mosaic virus (Au-CPMV) particles were generated by the electrostatic adsorption of positively charged electrolyte on the virus capsid with the subsequent incubation and reduction of anionic gold complexes to generate Au-CPMV particles. Au-CPMV particles as a CT contrast agent offer a fast scan time (less than 2 minutes) method, low cost, biocompatibility and a high-resolution image with ~ 150 Hounsfield Units (HU). The Au-CPMV surface was further modified allowing the incorporation of targeting molecules of specific cell types for imaging diseased cells.

AB - Biologically derived nanoparticles, such as proteins and viruses, provide unique platforms that allow the incorporation of novel properties due to their surface composition, their amenability for chemical and genetic engineering, monodispersity and biodegradability. Chemical modification of the virus surface, both the interior and the exterior, imparts new functionalities, that have potential applications in nanomedicine. In this study, we developed novel virus-based nanomaterials as a contrast agent for computed tomography (CT) imaging in vitro. The gold-coated cowpea mosaic virus (Au-CPMV) particles were generated by the electrostatic adsorption of positively charged electrolyte on the virus capsid with the subsequent incubation and reduction of anionic gold complexes to generate Au-CPMV particles. Au-CPMV particles as a CT contrast agent offer a fast scan time (less than 2 minutes) method, low cost, biocompatibility and a high-resolution image with ~ 150 Hounsfield Units (HU). The Au-CPMV surface was further modified allowing the incorporation of targeting molecules of specific cell types for imaging diseased cells.

KW - biomedical imaging; computed tomography (CT); gold; nanotechnology; viruses; targeting

KW - gold

KW - viruses

KW - targeting

KW - computed tomography (CT)

KW - nanotechnology

KW - biomedical imaging

U2 - 10.3762/bjnano.10.195

DO - 10.3762/bjnano.10.195

M3 - Article

VL - 10

SP - 1983

EP - 1993

JO - Beilstein Journal of Nanotechnology

T2 - Beilstein Journal of Nanotechnology

JF - Beilstein Journal of Nanotechnology

SN - 2190-4286

ER -

Aljabali AAA, Al Zoubi MS, Al-Batanyeh KM, Al-Radaideh A, Obeid MA, Al Sharabi A et al. Gold-coated plant virus as computed tomography imaging contrast agent. Beilstein Journal of Nanotechnology. 2019 Oct 7;10:1983-1993. https://doi.org/10.3762/bjnano.10.195