GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE

A TENGHOLM, Neville McClenaghan, E GRAPENGIESSER, E GYLFE, B HELLMAN

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Increase of the glucose concentration from 3 to 11 mM resulted in a triphasic release of insulin from perifused ob/ob-mouse beta-cells. A slight inhibition was followed after 2 min by a marked peak and a less pronounced sustained response. At the lower glucose concentration glycine had only marginal effects. However, in the presence of 11 mM glucose, 1-10 mM glycine triggered an immediate and dose-dependent response with an initial peak of insulin release followed by sustained stimulation. In individual beta-cells, rise of the glucose concentration from 3 to 11 mM induced initial lowering of the cytoplasmic Ca2+ concentration ([Ca2+]i) followed by large amplitude oscillations from a level of 50-90 nM to peak values exceeding 300 nM. Already at a concentration of 1 mM, glycine transformed the oscillatory pattern into a sustained level with increase of time-average [Ca2+]i. This elevation became more pronounced in the presence of 10 mM glycine. The effects of glycine on insulin release and [Ca2+]i required extracellular Na+ and were reproduced with the N-methyl analogue sarcosine. It is suggested that glycine potentiation of secretion reflects the elevation of time-average [Ca2+]i both by increased entry and reduced elimination of the cation from the cytoplasm.
LanguageEnglish
Pages243-247
JournalBIOCHIMICA ET BIOPHYSICA ACTA
Volume1137
Issue number3
Publication statusPublished - Nov 1992

Fingerprint

Glycine
Insulin
Glucose
Sarcosine
Cations
Cytoplasm

Cite this

TENGHOLM, A., McClenaghan, N., GRAPENGIESSER, E., GYLFE, E., & HELLMAN, B. (1992). GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE. BIOCHIMICA ET BIOPHYSICA ACTA, 1137(3), 243-247.
TENGHOLM, A ; McClenaghan, Neville ; GRAPENGIESSER, E ; GYLFE, E ; HELLMAN, B. / GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE. In: BIOCHIMICA ET BIOPHYSICA ACTA. 1992 ; Vol. 1137, No. 3. pp. 243-247.
@article{915c0bb422b94016bb6c18eb1360ea68,
title = "GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE",
abstract = "Increase of the glucose concentration from 3 to 11 mM resulted in a triphasic release of insulin from perifused ob/ob-mouse beta-cells. A slight inhibition was followed after 2 min by a marked peak and a less pronounced sustained response. At the lower glucose concentration glycine had only marginal effects. However, in the presence of 11 mM glucose, 1-10 mM glycine triggered an immediate and dose-dependent response with an initial peak of insulin release followed by sustained stimulation. In individual beta-cells, rise of the glucose concentration from 3 to 11 mM induced initial lowering of the cytoplasmic Ca2+ concentration ([Ca2+]i) followed by large amplitude oscillations from a level of 50-90 nM to peak values exceeding 300 nM. Already at a concentration of 1 mM, glycine transformed the oscillatory pattern into a sustained level with increase of time-average [Ca2+]i. This elevation became more pronounced in the presence of 10 mM glycine. The effects of glycine on insulin release and [Ca2+]i required extracellular Na+ and were reproduced with the N-methyl analogue sarcosine. It is suggested that glycine potentiation of secretion reflects the elevation of time-average [Ca2+]i both by increased entry and reduced elimination of the cation from the cytoplasm.",
author = "A TENGHOLM and Neville McClenaghan and E GRAPENGIESSER and E GYLFE and B HELLMAN",
year = "1992",
month = "11",
language = "English",
volume = "1137",
pages = "243--247",
journal = "BBA - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "3",

}

TENGHOLM, A, McClenaghan, N, GRAPENGIESSER, E, GYLFE, E & HELLMAN, B 1992, 'GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE', BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1137, no. 3, pp. 243-247.

GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE. / TENGHOLM, A; McClenaghan, Neville; GRAPENGIESSER, E; GYLFE, E; HELLMAN, B.

In: BIOCHIMICA ET BIOPHYSICA ACTA, Vol. 1137, No. 3, 11.1992, p. 243-247.

Research output: Contribution to journalArticle

TY - JOUR

T1 - GLYCINE TRANSFORMATION OF CA-2+ OSCILLATIONS INTO A SUSTAINED INCREASE PARALLELS POTENTIATION OF INSULIN RELEASE

AU - TENGHOLM, A

AU - McClenaghan, Neville

AU - GRAPENGIESSER, E

AU - GYLFE, E

AU - HELLMAN, B

PY - 1992/11

Y1 - 1992/11

N2 - Increase of the glucose concentration from 3 to 11 mM resulted in a triphasic release of insulin from perifused ob/ob-mouse beta-cells. A slight inhibition was followed after 2 min by a marked peak and a less pronounced sustained response. At the lower glucose concentration glycine had only marginal effects. However, in the presence of 11 mM glucose, 1-10 mM glycine triggered an immediate and dose-dependent response with an initial peak of insulin release followed by sustained stimulation. In individual beta-cells, rise of the glucose concentration from 3 to 11 mM induced initial lowering of the cytoplasmic Ca2+ concentration ([Ca2+]i) followed by large amplitude oscillations from a level of 50-90 nM to peak values exceeding 300 nM. Already at a concentration of 1 mM, glycine transformed the oscillatory pattern into a sustained level with increase of time-average [Ca2+]i. This elevation became more pronounced in the presence of 10 mM glycine. The effects of glycine on insulin release and [Ca2+]i required extracellular Na+ and were reproduced with the N-methyl analogue sarcosine. It is suggested that glycine potentiation of secretion reflects the elevation of time-average [Ca2+]i both by increased entry and reduced elimination of the cation from the cytoplasm.

AB - Increase of the glucose concentration from 3 to 11 mM resulted in a triphasic release of insulin from perifused ob/ob-mouse beta-cells. A slight inhibition was followed after 2 min by a marked peak and a less pronounced sustained response. At the lower glucose concentration glycine had only marginal effects. However, in the presence of 11 mM glucose, 1-10 mM glycine triggered an immediate and dose-dependent response with an initial peak of insulin release followed by sustained stimulation. In individual beta-cells, rise of the glucose concentration from 3 to 11 mM induced initial lowering of the cytoplasmic Ca2+ concentration ([Ca2+]i) followed by large amplitude oscillations from a level of 50-90 nM to peak values exceeding 300 nM. Already at a concentration of 1 mM, glycine transformed the oscillatory pattern into a sustained level with increase of time-average [Ca2+]i. This elevation became more pronounced in the presence of 10 mM glycine. The effects of glycine on insulin release and [Ca2+]i required extracellular Na+ and were reproduced with the N-methyl analogue sarcosine. It is suggested that glycine potentiation of secretion reflects the elevation of time-average [Ca2+]i both by increased entry and reduced elimination of the cation from the cytoplasm.

M3 - Article

VL - 1137

SP - 243

EP - 247

JO - BBA - General Subjects

T2 - BBA - General Subjects

JF - BBA - General Subjects

SN - 0304-4165

IS - 3

ER -