Geobacillus debilis sp nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

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Abstract

Several aerobic, motile, rod-shaped, thermophilic, spore-forming Geobacillus bacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and Tf-T, showed low 16S rRNA gene sequence similarity to recognized members of the genus Geobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and Tf-T represent a single novel species, for which the name Geobacillus debilis sp. nov. is proposed, with type strain Tf-T (= DSM 16016(T) =NCIMB 13995(T)) and which belongs to a subgroup of the genus Geobacillus comprising Geobacillus toebii and Geobacillus caldoxylosilyticus. However, G. debilis showed closest affinities to Bacillus pallidus, which we propose should become Geobacillus pallidus comb. nov.
LanguageEnglish
Pages2197-2201
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume54
Issue numberPart 6
DOIs
Publication statusPublished - Nov 2004

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thermophilic bacterium
spore
phylogenetics
bacterium
gene
soil
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@article{4d73143c4e514d498c5814e2a77c5a33,
title = "Geobacillus debilis sp nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.",
abstract = "Several aerobic, motile, rod-shaped, thermophilic, spore-forming Geobacillus bacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and Tf-T, showed low 16S rRNA gene sequence similarity to recognized members of the genus Geobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and Tf-T represent a single novel species, for which the name Geobacillus debilis sp. nov. is proposed, with type strain Tf-T (= DSM 16016(T) =NCIMB 13995(T)) and which belongs to a subgroup of the genus Geobacillus comprising Geobacillus toebii and Geobacillus caldoxylosilyticus. However, G. debilis showed closest affinities to Bacillus pallidus, which we propose should become Geobacillus pallidus comb. nov.",
author = "Ibrahim Banat and Roger Marchant and TJ Rahman",
year = "2004",
month = "11",
doi = "10.1099/ijs.0.63231-0",
language = "English",
volume = "54",
pages = "2197--2201",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
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TY - JOUR

T1 - Geobacillus debilis sp nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov.

AU - Banat, Ibrahim

AU - Marchant, Roger

AU - Rahman, TJ

PY - 2004/11

Y1 - 2004/11

N2 - Several aerobic, motile, rod-shaped, thermophilic, spore-forming Geobacillus bacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and Tf-T, showed low 16S rRNA gene sequence similarity to recognized members of the genus Geobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and Tf-T represent a single novel species, for which the name Geobacillus debilis sp. nov. is proposed, with type strain Tf-T (= DSM 16016(T) =NCIMB 13995(T)) and which belongs to a subgroup of the genus Geobacillus comprising Geobacillus toebii and Geobacillus caldoxylosilyticus. However, G. debilis showed closest affinities to Bacillus pallidus, which we propose should become Geobacillus pallidus comb. nov.

AB - Several aerobic, motile, rod-shaped, thermophilic, spore-forming Geobacillus bacteria predominantly giving a Gram-positive staining reaction were isolated from a cool soil environment in Northern Ireland and taxonomically investigated. Two isolates, F10 and Tf-T, showed low 16S rRNA gene sequence similarity to recognized members of the genus Geobacillus. Phylogenetic tree investigation using neighbour-joining, maximum-likelihood and parsimony methods indicated that strains F10 and Tf-T represent a single novel species, for which the name Geobacillus debilis sp. nov. is proposed, with type strain Tf-T (= DSM 16016(T) =NCIMB 13995(T)) and which belongs to a subgroup of the genus Geobacillus comprising Geobacillus toebii and Geobacillus caldoxylosilyticus. However, G. debilis showed closest affinities to Bacillus pallidus, which we propose should become Geobacillus pallidus comb. nov.

U2 - 10.1099/ijs.0.63231-0

DO - 10.1099/ijs.0.63231-0

M3 - Article

VL - 54

SP - 2197

EP - 2201

JO - International Journal of Systematic and Evolutionary Microbiology

T2 - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - Part 6

ER -