Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland

Heather P. Thompson, James Dooley, John Kenny, Maurice McCoy, Colm Lowery, John E. Moore, Lihua Xiao

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Cryptosporidium spp. in diarrheic calves less than 30 days old from farms across Northern Ireland were examined over a year period by microscopic, genotyping, and subtyping techniques to characterize the transmission dynamics. Cryptosporidium oocysts were detected in 291 of 779 (37.4%) animals. The prevalence rates of rotavirus, coronavirus, and Escherichia coli K99+ were lower as seen in 242 of 806 (30.0%), 46/806 (5.7%), and 16/421 (3.8%) of animals, respectively. Of the 224 Cryptosporidium-positive specimens available for molecular analysis, Cryptosporidium parvum was identified in 213 (95.1%) specimens, Cryptosporidium bovis in eight (3.6%), and Cryptosporidium deer-like genotype in three (1.3%). Sequence analysis of the 60-kDa glycoprotein gene identified 16 IIa subtypes and a new subtype family, with 120 of the 216 (55.6%) positive specimens having the subtype IIaA18G3R1. Eight of the IIa subtypes were previously seen in humans in Northern Ireland. Several subtypes were temporally or geographically unique. The genetic diversity in calves in Northern Ireland was much greater than that reported from other areas. This work demonstrates the utility of genotyping and subtyping tools in characterizing the transmission of Cryptosporidium spp. in calves and humans.
LanguageEnglish
Pages619-624
JournalParasitology Research
Volume100
Issue number3
DOIs
Publication statusPublished - Feb 2007

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Northern Ireland
Cryptosporidium
calves
genotype
genotyping
Cryptosporidium bovis
Coronavirinae
Cryptosporidium parvum
Rotavirus
oocysts
deer
glycoproteins
animals
sequence analysis
Escherichia coli
farms
genetic variation
genes

Cite this

Thompson, Heather P. ; Dooley, James ; Kenny, John ; McCoy, Maurice ; Lowery, Colm ; Moore, John E. ; Xiao, Lihua. / Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland. In: Parasitology Research. 2007 ; Vol. 100, No. 3. pp. 619-624.
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abstract = "Cryptosporidium spp. in diarrheic calves less than 30 days old from farms across Northern Ireland were examined over a year period by microscopic, genotyping, and subtyping techniques to characterize the transmission dynamics. Cryptosporidium oocysts were detected in 291 of 779 (37.4{\%}) animals. The prevalence rates of rotavirus, coronavirus, and Escherichia coli K99+ were lower as seen in 242 of 806 (30.0{\%}), 46/806 (5.7{\%}), and 16/421 (3.8{\%}) of animals, respectively. Of the 224 Cryptosporidium-positive specimens available for molecular analysis, Cryptosporidium parvum was identified in 213 (95.1{\%}) specimens, Cryptosporidium bovis in eight (3.6{\%}), and Cryptosporidium deer-like genotype in three (1.3{\%}). Sequence analysis of the 60-kDa glycoprotein gene identified 16 IIa subtypes and a new subtype family, with 120 of the 216 (55.6{\%}) positive specimens having the subtype IIaA18G3R1. Eight of the IIa subtypes were previously seen in humans in Northern Ireland. Several subtypes were temporally or geographically unique. The genetic diversity in calves in Northern Ireland was much greater than that reported from other areas. This work demonstrates the utility of genotyping and subtyping tools in characterizing the transmission of Cryptosporidium spp. in calves and humans.",
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Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland. / Thompson, Heather P.; Dooley, James; Kenny, John; McCoy, Maurice; Lowery, Colm; Moore, John E.; Xiao, Lihua.

In: Parasitology Research, Vol. 100, No. 3, 02.2007, p. 619-624.

Research output: Contribution to journalArticle

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