Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome

Guillaume Borrel, Angela McCann, Jennifer Deane, Marta C Neto, Denise B Lynch, Jean- Francois Brugere, Paul W O' Toole

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The biological significance of Archaea in the human gut microbiota is largely unclear. We recently reported genomic and biochemical analyses of the Methanomassiliicoccales, a novel order of methanogenic Archaea dwelling in soil and the animal digestive tract. We now show that these Methanomassiliicoccales are present in published microbiome data sets from eight countries. They are represented by five Operational Taxonomic Units present in at least four cohorts and phylogenetically distributed into two clades. Genes for utilizing trimethylamine (TMA), a bacterial precursor to an atherosclerogenic human metabolite, were present in four of the six novel Methanomassiliicoccales genomes assembled from ELDERMET metagenomes. In addition to increased microbiota TMA production capacity in long-term residential care subjects, abundance of TMA-utilizing Methanomassiliicoccales correlated positively with bacterial gene count for TMA production and negatively with fecal TMA concentrations. The two large Methanomassiliicoccales clades have opposite correlations with host health status in the ELDERMET cohort and putative distinct genomic signatures for gut adaptation.
LanguageEnglish
Pages2059-2074
JournalISME Journal
Volume11
Early online date6 Jun 2017
DOIs
Publication statusE-pub ahead of print - 6 Jun 2017

Fingerprint

Metagenomics
Microbiota
Archaea
Genomics
Metagenome
Bacterial Genes
Bacterial Load
Long-Term Care
Health Status
Gastrointestinal Tract
Soil
Gastrointestinal Microbiome
trimethylamine
Genome
Genes

Keywords

  • Human gut microbiome
  • archaea
  • genomics
  • metagenomics

Cite this

Borrel, G., McCann, A., Deane, J., Neto, M. C., Lynch, D. B., Brugere, J. F., & O' Toole, P. W. (2017). Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome. ISME Journal, 11, 2059-2074. https://doi.org/10.1038/ismej.2017.72
Borrel, Guillaume ; McCann, Angela ; Deane, Jennifer ; Neto, Marta C ; Lynch, Denise B ; Brugere, Jean- Francois ; O' Toole, Paul W. / Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome. In: ISME Journal. 2017 ; Vol. 11. pp. 2059-2074.
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Borrel, G, McCann, A, Deane, J, Neto, MC, Lynch, DB, Brugere, JF & O' Toole, PW 2017, 'Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome', ISME Journal, vol. 11, pp. 2059-2074. https://doi.org/10.1038/ismej.2017.72

Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome. / Borrel, Guillaume; McCann, Angela; Deane, Jennifer; Neto, Marta C; Lynch, Denise B; Brugere, Jean- Francois; O' Toole, Paul W.

In: ISME Journal, Vol. 11, 06.06.2017, p. 2059-2074.

Research output: Contribution to journalArticle

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Borrel G, McCann A, Deane J, Neto MC, Lynch DB, Brugere JF et al. Genomics and metagenomics of trimethylamine-utilizing Archaea in the human gut microbiome. ISME Journal. 2017 Jun 6;11:2059-2074. https://doi.org/10.1038/ismej.2017.72