Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S

Efstathios S. Giotis, Arunachalam Muthaiyan, Ian S. Blair, Brian J. Wilkinson, DA McDowell

    Research output: Non-textual formWeb publication/site

    32 Citations (Scopus)

    Abstract

    Abstract: Background: Information regarding the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes is very limited. Treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the AlTR is at least partially protein-dependent. In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively.Results: In this study, L. monocytogenes was shown to exhibit a significant AlTR following a 1-h exposure to mild alkali (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Adaptive intracellular gene expression involved genes that are associated with virulence, the general stress response, cell division, and changes in cell wall structure and included many genes with unknown functions. The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. Interestingly, several alkali induced genes/proteins can provide a cross protective overlap to other types of stresses.Conclusion: Alkali pH provides therefore L. monocytogenes with nonspecific multiple-stress resistance that may be vital for survival in the human gastrointestinal tract as well as within food processing systems where alkali conditions prevail. This study showed strong evidence that the AlTR in L. monocytogenes functions as to minimize excess alkalisation and energy expenditures while mobilizing available carbon sources.
    LanguageEnglish
    DOIs
    Publication statusPublished - Jun 2008

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    alkalis
    proteomics
    Listeria monocytogenes
    genomics
    complementary DNA
    cells
    alkali treatment
    alkalinization
    protein synthesis inhibitors
    gene expression
    genes
    two-dimensional gel electrophoresis
    post-translational modification
    chloramphenicol
    lethal genes
    food processing
    energy expenditure
    stress tolerance
    gel electrophoresis
    gastrointestinal system

    Cite this

    Giotis, E. S. (Author), Muthaiyan, A. (Author), Blair, I. S. (Author), Wilkinson, B. J. (Author), & McDowell, DA. (Author). (2008). Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S. Web publication/site https://doi.org/10.1186/1471-2180-8-102
    Giotis, Efstathios S. (Author) ; Muthaiyan, Arunachalam (Author) ; Blair, Ian S. (Author) ; Wilkinson, Brian J. (Author) ; McDowell, DA (Author). / Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S. [Web publication/site].
    @misc{2a5293df7d554d8c93d7c26f6c602c09,
    title = "Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S",
    abstract = "Abstract: Background: Information regarding the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes is very limited. Treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the AlTR is at least partially protein-dependent. In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively.Results: In this study, L. monocytogenes was shown to exhibit a significant AlTR following a 1-h exposure to mild alkali (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Adaptive intracellular gene expression involved genes that are associated with virulence, the general stress response, cell division, and changes in cell wall structure and included many genes with unknown functions. The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. Interestingly, several alkali induced genes/proteins can provide a cross protective overlap to other types of stresses.Conclusion: Alkali pH provides therefore L. monocytogenes with nonspecific multiple-stress resistance that may be vital for survival in the human gastrointestinal tract as well as within food processing systems where alkali conditions prevail. This study showed strong evidence that the AlTR in L. monocytogenes functions as to minimize excess alkalisation and energy expenditures while mobilizing available carbon sources.",
    author = "Giotis, {Efstathios S.} and Arunachalam Muthaiyan and Blair, {Ian S.} and Wilkinson, {Brian J.} and DA McDowell",
    year = "2008",
    month = "6",
    doi = "10.1186/1471-2180-8-102",
    language = "English",

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    Giotis, ES, Muthaiyan, A, Blair, IS, Wilkinson, BJ & McDowell, DA, Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S, 2008, Web publication/site. https://doi.org/10.1186/1471-2180-8-102
    Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S. Giotis, Efstathios S. (Author); Muthaiyan, Arunachalam (Author); Blair, Ian S. (Author); Wilkinson, Brian J. (Author); McDowell, DA (Author). 2008.

    Research output: Non-textual formWeb publication/site

    TY - ADVS

    T1 - Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S

    AU - Giotis, Efstathios S.

    AU - Muthaiyan, Arunachalam

    AU - Blair, Ian S.

    AU - Wilkinson, Brian J.

    AU - McDowell, DA

    PY - 2008/6

    Y1 - 2008/6

    N2 - Abstract: Background: Information regarding the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes is very limited. Treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the AlTR is at least partially protein-dependent. In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively.Results: In this study, L. monocytogenes was shown to exhibit a significant AlTR following a 1-h exposure to mild alkali (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Adaptive intracellular gene expression involved genes that are associated with virulence, the general stress response, cell division, and changes in cell wall structure and included many genes with unknown functions. The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. Interestingly, several alkali induced genes/proteins can provide a cross protective overlap to other types of stresses.Conclusion: Alkali pH provides therefore L. monocytogenes with nonspecific multiple-stress resistance that may be vital for survival in the human gastrointestinal tract as well as within food processing systems where alkali conditions prevail. This study showed strong evidence that the AlTR in L. monocytogenes functions as to minimize excess alkalisation and energy expenditures while mobilizing available carbon sources.

    AB - Abstract: Background: Information regarding the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes is very limited. Treatment of alkali-adapted cells with the protein synthesis inhibitor chloramphenicol has revealed that the AlTR is at least partially protein-dependent. In order to gain a more comprehensive perspective on the physiology and regulation of the AlTR, we compared differential gene expression and protein content of cells adapted at pH 9.5 and un-adapted cells (pH 7.0) using complementary DNA (cDNA) microarray and two-dimensional (2D) gel electrophoresis, (combined with mass spectrometry) respectively.Results: In this study, L. monocytogenes was shown to exhibit a significant AlTR following a 1-h exposure to mild alkali (pH 9.5), which is capable of protecting cells from subsequent lethal alkali stress (pH 12.0). Adaptive intracellular gene expression involved genes that are associated with virulence, the general stress response, cell division, and changes in cell wall structure and included many genes with unknown functions. The observed variability between results of cDNA arrays and 2D gel electrophoresis may be accounted for by posttranslational modifications. Interestingly, several alkali induced genes/proteins can provide a cross protective overlap to other types of stresses.Conclusion: Alkali pH provides therefore L. monocytogenes with nonspecific multiple-stress resistance that may be vital for survival in the human gastrointestinal tract as well as within food processing systems where alkali conditions prevail. This study showed strong evidence that the AlTR in L. monocytogenes functions as to minimize excess alkalisation and energy expenditures while mobilizing available carbon sources.

    U2 - 10.1186/1471-2180-8-102

    DO - 10.1186/1471-2180-8-102

    M3 - Web publication/site

    ER -

    Giotis ES (Author), Muthaiyan A (Author), Blair IS (Author), Wilkinson BJ (Author), McDowell DA (Author). Genomic and proteomic analysis of the Alkali-Tolerance Response (AlTR) in Listeria monocytogenes 10403S 2008. https://doi.org/10.1186/1471-2180-8-102