Generation of [Ala1, Thr6]Bradykinin in the Plasma of a Snake, the Reticulated Python

J. Michael Conlon, Valentine A. Lance

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Incubation of heat-denatured plasma from the reticulated python, Python reticulatus with trypsin generated bradykinin-like immunoreactivity which was detected by radioimmunoassay using an antiserum raised against mammalian bradykinin. The primary structure of python bradykinin was established as Ala-Pro-Pre-Gly-Phe-Thr-Pro-Phe-Arg. This amino acid sequence contains two substitutions (Arg1 → Ala and Ser6 → Thr) compared with mammalian bradykinin. Treatment of python plasma with glass beads under conditions previously shown to generate [Thr6]bradykinin in the plasma of a turtle and an alligator did not generate bradykinin-like immunoreactivity. The data indicate that the plasma of a lepidosaur contains a kininogen but, unlike the plasma of chelonians and crocodilians, does not contain a prekallikrein activator related to Factor XII.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalGeneral and Comparative Endocrinology
Issue number2
Publication statusPublished (in print/issue) - May 1994


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