Gastrointestinal modifications and bioavailability of brown seaweed phlorotannins and effects on inflammatory markers

Giulia Corona, Yang Ji, Prapaporn Anegboonlap, Sarah Hotchkiss, C Gill, Parveen Yaqoob, Jeremy P. E. Spencer, Ian Rowland

Research output: Contribution to journalArticle

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Abstract

Brown seaweeds such as Ascophyllum nodosum are a rich source of phlorotannins (oligomers and polymers of phloroglucinol units), a class of polyphenols that are unique to Phaeophyceae. At present, there is no information on the bioavailability of seaweed polyphenols and limited evidence on their bioactivity in vivo. Consequently, we investigated the gastrointestinal modifications in vitro of seaweed phlorotannins from A. nodosum and their bioavailability and effect on inflammatory markers in healthy participants. In vitro, some phlorotannin oligomers were identified after digestion and colonic fermentation. In addition, seven metabolites corresponding to in vitro-absorbed metabolites were identified. Urine and plasma samples contained a variety of metabolites attributed to both unconjugated and conjugated metabolites (glucuronides and/or sulphates). In both urine and plasma, the majority of the metabolites were found in samples collected at late time points (6–24 h), suggesting colonic metabolism of high-molecular-weight phlorotannins, with three phlorotannin oligomers (hydroxytrifuhalol A, 7-hydroxyeckol, C-O-C dimer of phloroglucinol) identified in urine samples. A significant increase of the cytokine IL-8 was also observed. Our study shows for the first time that seaweed phlorotannins are metabolised and absorbed, predominantly in the large intestine, and there is a large inter-individual variation in their metabolic profile. Three phlorotannin oligomers present in the capsule are excreted in urine. Our study is the first investigation of the metabolism and bioavailability of seaweed phlorotannins and the role of colonic biotransformation. In addition, IL-8 is a possible target for phlorotannin bioactivity.

Original languageEnglish
Pages (from-to)1240-1253
Number of pages14
JournalBritish Journal of Nutrition
Volume115
Issue number7
Early online date16 Feb 2016
DOIs
Publication statusPublished - Apr 2016

Fingerprint

Seaweed
Biological Availability
Phloroglucinol
Urine
Polyphenols
Interleukin-8
Ascophyllum
Phaeophyta
Metabolome
Large Intestine
Glucuronides
Biotransformation
Sulfates
Fermentation
Capsules
Digestion
Healthy Volunteers
Polymers
Molecular Weight
Cytokines

Keywords

  • Human subjects
  • Metabolism
  • Polyphenols
  • Phlorotannins
  • Brown seaweed
  • Bioavailability

Cite this

Corona, Giulia ; Ji, Yang ; Anegboonlap, Prapaporn ; Hotchkiss, Sarah ; Gill, C ; Yaqoob, Parveen ; Spencer, Jeremy P. E. ; Rowland, Ian. / Gastrointestinal modifications and bioavailability of brown seaweed phlorotannins and effects on inflammatory markers. In: British Journal of Nutrition. 2016 ; Vol. 115, No. 7. pp. 1240-1253.
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Gastrointestinal modifications and bioavailability of brown seaweed phlorotannins and effects on inflammatory markers. / Corona, Giulia; Ji, Yang; Anegboonlap, Prapaporn; Hotchkiss, Sarah; Gill, C; Yaqoob, Parveen; Spencer, Jeremy P. E.; Rowland, Ian.

In: British Journal of Nutrition, Vol. 115, No. 7, 04.2016, p. 1240-1253.

Research output: Contribution to journalArticle

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AU - Corona, Giulia

AU - Ji, Yang

AU - Anegboonlap, Prapaporn

AU - Hotchkiss, Sarah

AU - Gill, C

AU - Yaqoob, Parveen

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AB - Brown seaweeds such as Ascophyllum nodosum are a rich source of phlorotannins (oligomers and polymers of phloroglucinol units), a class of polyphenols that are unique to Phaeophyceae. At present, there is no information on the bioavailability of seaweed polyphenols and limited evidence on their bioactivity in vivo. Consequently, we investigated the gastrointestinal modifications in vitro of seaweed phlorotannins from A. nodosum and their bioavailability and effect on inflammatory markers in healthy participants. In vitro, some phlorotannin oligomers were identified after digestion and colonic fermentation. In addition, seven metabolites corresponding to in vitro-absorbed metabolites were identified. Urine and plasma samples contained a variety of metabolites attributed to both unconjugated and conjugated metabolites (glucuronides and/or sulphates). In both urine and plasma, the majority of the metabolites were found in samples collected at late time points (6–24 h), suggesting colonic metabolism of high-molecular-weight phlorotannins, with three phlorotannin oligomers (hydroxytrifuhalol A, 7-hydroxyeckol, C-O-C dimer of phloroglucinol) identified in urine samples. A significant increase of the cytokine IL-8 was also observed. Our study shows for the first time that seaweed phlorotannins are metabolised and absorbed, predominantly in the large intestine, and there is a large inter-individual variation in their metabolic profile. Three phlorotannin oligomers present in the capsule are excreted in urine. Our study is the first investigation of the metabolism and bioavailability of seaweed phlorotannins and the role of colonic biotransformation. In addition, IL-8 is a possible target for phlorotannin bioactivity.

KW - Human subjects

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