Ganoderma lucidum polysaccharide loaded sodium alginate micro-particles prepared via electrospraying in controlled deposition environments

Zhi Cheng Yao, Li Jie Jin, Zeeshan Ahmad, Jie Huang, Ming Wei Chang, Jing Song Li

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ganoderma lucidum polysaccharide (GLP) is a functional food source deployed in preventative medicine. However, applications utilizing GLP are limited due to oxidative and acidic environmental damage. Advances in preserving GLP structure (and therefore function), in situ, will diversify their applications within biomedical fields (drug and antibacterial active delivery via the enteral route). In this study, GLP loaded sodium alginate (NaAlg) micro-particles (size range 225–355 μm) were generated using the electrospray (ES) process. The loading capacity and encapsulation efficiency of GLP for composite particles (collected at different temperatures) were ∼23% and 71%, respectively. The collection substrate (CaCl2, 1–20 w/v%) concentration was explored and preliminary findings indicated a 10 w/v% solution to be optimal. The process was further modified by manipulating the collection environment temperature (∼25 to 50 °C). Based on this, NaAlg/GLP micro-particles were engineered with variable surface morphologies (porous and crinkled), without effecting the chemical composition of either material (GLP and NaAlg). In-vitro release studies demonstrated pH responsive release rates. Modest release of GLP from micro-particles in simulated gastric fluid (pH ∼1.7) was observed, while rapid release was exhibited under simulated intestinal conditions (pH ∼7.4). Release of GLP from NaAlg beads was the greatest from samples prepared at elevated environmental temperatures. These findings demonstrate a facile route to fabricate GLP-NaAlg loaded micro-particles with various shapes, surface topographies and release characteristics via a one-step ES process.

LanguageEnglish
Pages148-158
Number of pages11
JournalInternational Journal of Pharmaceutics
Volume524
Issue number1-2
Early online date28 Mar 2017
DOIs
Publication statusPublished - 30 May 2017

Fingerprint

Reishi
Controlled Environment
Polysaccharides
Temperature
alginic acid
Functional Food
Preventive Medicine
Particle Size
Small Intestine
Stomach

Keywords

  • Cross-linking
  • Encapsulation
  • Food
  • Oral
  • Temperature regulation

Cite this

Yao, Zhi Cheng ; Jin, Li Jie ; Ahmad, Zeeshan ; Huang, Jie ; Chang, Ming Wei ; Li, Jing Song. / Ganoderma lucidum polysaccharide loaded sodium alginate micro-particles prepared via electrospraying in controlled deposition environments. 2017 ; Vol. 524, No. 1-2. pp. 148-158.
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abstract = "Ganoderma lucidum polysaccharide (GLP) is a functional food source deployed in preventative medicine. However, applications utilizing GLP are limited due to oxidative and acidic environmental damage. Advances in preserving GLP structure (and therefore function), in situ, will diversify their applications within biomedical fields (drug and antibacterial active delivery via the enteral route). In this study, GLP loaded sodium alginate (NaAlg) micro-particles (size range 225–355 μm) were generated using the electrospray (ES) process. The loading capacity and encapsulation efficiency of GLP for composite particles (collected at different temperatures) were ∼23{\%} and 71{\%}, respectively. The collection substrate (CaCl2, 1–20 w/v{\%}) concentration was explored and preliminary findings indicated a 10 w/v{\%} solution to be optimal. The process was further modified by manipulating the collection environment temperature (∼25 to 50 °C). Based on this, NaAlg/GLP micro-particles were engineered with variable surface morphologies (porous and crinkled), without effecting the chemical composition of either material (GLP and NaAlg). In-vitro release studies demonstrated pH responsive release rates. Modest release of GLP from micro-particles in simulated gastric fluid (pH ∼1.7) was observed, while rapid release was exhibited under simulated intestinal conditions (pH ∼7.4). Release of GLP from NaAlg beads was the greatest from samples prepared at elevated environmental temperatures. These findings demonstrate a facile route to fabricate GLP-NaAlg loaded micro-particles with various shapes, surface topographies and release characteristics via a one-step ES process.",
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Ganoderma lucidum polysaccharide loaded sodium alginate micro-particles prepared via electrospraying in controlled deposition environments. / Yao, Zhi Cheng; Jin, Li Jie; Ahmad, Zeeshan; Huang, Jie; Chang, Ming Wei; Li, Jing Song.

Vol. 524, No. 1-2, 30.05.2017, p. 148-158.

Research output: Contribution to journalArticle

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AU - Yao, Zhi Cheng

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AU - Ahmad, Zeeshan

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AU - Chang, Ming Wei

AU - Li, Jing Song

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