Almost 80% of chronic wounds have a bacterial biofilm present. These wound biofilms are caused by a range of organisms and are often polymicrobial. Pseudomonas aeruginosa is one of the most common causative organisms in wound infections and readily forms biofilms in wounds. To coordinate this, P. aeruginosa uses a process known as quorum sensing. Structural homologues of the quorum sensing signalling molecules have been used to disrupt this communication and prevent biofilm formation by Pseudomonas. However, these compounds have not yet reached clinical use. Here, we report the production and characterisation of a lyophilised PVA aerogel for use in delivering furanones to wound biofilms. PVA aerogels successfully release a model antimicrobial and two naturally occurring furanones in an aqueous environment. Furanone loaded aerogels inhibited biofilm formation in P. aeruginosa by up to 98.80%. Further, furanone loaded aerogels successfully reduced total biomass of preformed biofilms. Treatment with a sotolon loaded aerogel yielded a 5.16 log reduction in viable biofilm bound cells in a novel model of chronic wound biofilm, equivalent to the current wound therapy Aquacel AG. These results highlight the potential utility of aerogels in drug delivery to infected wounds and supports the use of biofilm inhibitory compounds as wound therapeutics.
|Number of pages||10|
|Early online date||5 May 2023|
|Publication status||Published online - 5 May 2023|
Bibliographical noteFunding Information:
RRMC is supported by a Biotechnology and Biological Sciences Research Council New Investigator Award BB/V007823/1. RRMC and CP are supported by the Academy of Medical Sciences/the Wellcome Trust/the Government Department of Business, Energy and Industrial Strategy/the British Heart Foundation/Diabetes UK Springboard Award [SBF006\1040].
RRMC is supported by a Biotechnology and Biological Sciences Research Council New Investigator Award BB/V007823/1 . RRMC and CP are supported by the Academy of Medical Sciences /the Wellcome Trust /the Government Department of Business, Energy and Industrial Strategy /the British Heart Foundation / Diabetes UK Springboard Award [ SBF006\1040 ].
© 2023 The Authors
- wound model
- biofilm inhibition
- pseudomonas aeruginosa