From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

Clifford Barnes, George McKerr, A. M. Soto, Stephen Downes, Vyvyan Howard, Kurt Saetzler

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.
Original languageEnglish
Pages (from-to)1-12
JournalPLoS ONE
Volume9
Issue number4
Publication statusPublished - 1 Apr 2014

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