From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

Clifford Barnes, George McKerr, A. M. Soto, Stephen Downes, Vyvyan Howard, Kurt Saetzler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.
LanguageEnglish
Pages1-12
JournalPLoS ONE
Volume9
Issue number4
Publication statusPublished - 1 Apr 2014

Fingerprint

Morphogenesis
Breast
Human Mammary Glands
Collagen
Gels
Epithelium
Epithelial Cells

Cite this

Barnes, C., McKerr, G., Soto, A. M., Downes, S., Howard, V., & Saetzler, K. (2014). From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time. PLoS ONE, 9(4), 1-12.
Barnes, Clifford ; McKerr, George ; Soto, A. M. ; Downes, Stephen ; Howard, Vyvyan ; Saetzler, Kurt. / From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time. In: PLoS ONE. 2014 ; Vol. 9, No. 4. pp. 1-12.
@article{c5da85faab894ac984783f146c10d3f1,
title = "From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time",
abstract = "Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.",
author = "Clifford Barnes and George McKerr and Soto, {A. M.} and Stephen Downes and Vyvyan Howard and Kurt Saetzler",
year = "2014",
month = "4",
day = "1",
language = "English",
volume = "9",
pages = "1--12",
journal = "PLoS ONE",
issn = "1932-6203",
number = "4",

}

Barnes, C, McKerr, G, Soto, AM, Downes, S, Howard, V & Saetzler, K 2014, 'From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time', PLoS ONE, vol. 9, no. 4, pp. 1-12.

From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time. / Barnes, Clifford; McKerr, George; Soto, A. M.; Downes, Stephen; Howard, Vyvyan; Saetzler, Kurt.

In: PLoS ONE, Vol. 9, No. 4, 01.04.2014, p. 1-12.

Research output: Contribution to journalArticle

TY - JOUR

T1 - From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time

AU - Barnes, Clifford

AU - McKerr, George

AU - Soto, A. M.

AU - Downes, Stephen

AU - Howard, Vyvyan

AU - Saetzler, Kurt

PY - 2014/4/1

Y1 - 2014/4/1

N2 - Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.

AB - Mammary gland morphogenesis involves ductal elongation, branching, and budding. All of these processes are mediated by stroma - epithelium interactions. Biomechanical factors, such as matrix stiffness, have been established as important factors in these interactions. For example, epithelial cells fail to form normal acinar structures in vitro in 3D gels that exceed the stiffness of a normal mammary gland. Additionally, heterogeneity in the spatial distribution of acini and ducts within individual collagen gels suggests that local organization of the matrix may guide morphogenesis. Here, we quantified the effects of both bulk material stiffness and local collagen fiber arrangement on epithelial morphogenesis.

M3 - Article

VL - 9

SP - 1

EP - 12

JO - PLoS ONE

T2 - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 4

ER -

Barnes C, McKerr G, Soto AM, Downes S, Howard V, Saetzler K. From Single Cells to Tissues: Interactions between the Matrix and Human Breast Cells in Real Time. PLoS ONE. 2014 Apr 1;9(4):1-12.