Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material

O Elezi, Y Kourkoutas, AA Koutinas, M Kanellaki, E Bezirtzoglou, YA Barnett, Poonam Singh - Nee Nigam

Research output: Contribution to journalArticle

23 Citations (Scopus)


Improvements in yield and productivity in lactic acid fermentation by Lactobaccilus brevis cells immobilized on delignified cellulosic (DC) material are reported. The system proved to be more efficient in comparison with the work reported by other workers. Yields of 80 and 100% conversion using glucose were obtained at 30 degreesC in 1 day of fermentation time. Lactic acid fermentation using whey as substrate was obtained at 30 degreesC in 1-1.5 days, resulting in 70% yield, whereas the remaining lactose in whey was converted to alcohol byproduct, leading to a 90% lactose exploitation and 100% conversion. Cell immobilization of L. brevis on DC material was proved by its reuses in repeated batch fermentations and through electron microscopy. A series of 10 repeated batch fermentations without any loss in cell activity showed a tendency for high operational stability. The presence of DC material resulted in a drastic drop of the fermentation time from 48 to 13 h.
Original languageEnglish
Pages (from-to)5285-5289
JournalJournal of Agricultural and Food Chemistry
Issue number18
Publication statusPublished - Aug 2003


Cite this