Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material

O Elezi, Y Kourkoutas, AA Koutinas, M Kanellaki, E Bezirtzoglou, YA Barnett, Poonam Singh - Nee Nigam

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Improvements in yield and productivity in lactic acid fermentation by Lactobaccilus brevis cells immobilized on delignified cellulosic (DC) material are reported. The system proved to be more efficient in comparison with the work reported by other workers. Yields of 80 and 100% conversion using glucose were obtained at 30 degreesC in 1 day of fermentation time. Lactic acid fermentation using whey as substrate was obtained at 30 degreesC in 1-1.5 days, resulting in 70% yield, whereas the remaining lactose in whey was converted to alcohol byproduct, leading to a 90% lactose exploitation and 100% conversion. Cell immobilization of L. brevis on DC material was proved by its reuses in repeated batch fermentations and through electron microscopy. A series of 10 repeated batch fermentations without any loss in cell activity showed a tendency for high operational stability. The presence of DC material resulted in a drastic drop of the fermentation time from 48 to 13 h.
LanguageEnglish
Pages5285-5289
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number18
DOIs
Publication statusPublished - Aug 2003

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cellulosic materials
Lactobacillus brevis
immobilized cells
food additives
lactic acid
lactic fermentation
batch fermentation
whey
lactose
fermentation
byproducts
electron microscopy
alcohols
cells
glucose

Cite this

Elezi, O ; Kourkoutas, Y ; Koutinas, AA ; Kanellaki, M ; Bezirtzoglou, E ; Barnett, YA ; Singh - Nee Nigam, Poonam. / Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material. In: Journal of Agricultural and Food Chemistry. 2003 ; Vol. 51, No. 18. pp. 5285-5289.
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abstract = "Improvements in yield and productivity in lactic acid fermentation by Lactobaccilus brevis cells immobilized on delignified cellulosic (DC) material are reported. The system proved to be more efficient in comparison with the work reported by other workers. Yields of 80 and 100{\%} conversion using glucose were obtained at 30 degreesC in 1 day of fermentation time. Lactic acid fermentation using whey as substrate was obtained at 30 degreesC in 1-1.5 days, resulting in 70{\%} yield, whereas the remaining lactose in whey was converted to alcohol byproduct, leading to a 90{\%} lactose exploitation and 100{\%} conversion. Cell immobilization of L. brevis on DC material was proved by its reuses in repeated batch fermentations and through electron microscopy. A series of 10 repeated batch fermentations without any loss in cell activity showed a tendency for high operational stability. The presence of DC material resulted in a drastic drop of the fermentation time from 48 to 13 h.",
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Food additive lactic acid production by immobilized cells of Lactobacillus brevis on delignified cellulosic material. / Elezi, O; Kourkoutas, Y; Koutinas, AA; Kanellaki, M; Bezirtzoglou, E; Barnett, YA; Singh - Nee Nigam, Poonam.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 18, 08.2003, p. 5285-5289.

Research output: Contribution to journalArticle

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