FMRFamide-related peptides in potato cyst nematodes

MJ Kimber, CC Fleming, AJ Bjourson, DW Halton, AG Maule

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

This study presents data demonstrating the presence of FMRFamide-related peptides (FaRPs) in potato cyst nematodes (PCN). Five transcripts of FaRP encoding genes, designated gpflp-1 to gpflp-5, were characterised using RACE. In terms of ORFs, gpflp-1 was 444 base pairs (bp) long and coded for four copies of the FaRP, PF3 (KSAYMRFamide) whilst gpflp-2 was 309 bp long and encoded one copy of the peptide, KNKFEFIRFamide. gpflp-3 (420 bp) Encoded two copies of KHEYLRFamide (AF2) and the genes gpflp-4 and gpflp-5 encoded a total of 11 FaRPs, most of which are novel to PCN. FMRFamide-related peptide (FaRP)-like immunoreactivity was observed in both PCN species, Globodera pallida and Globodera rostochiensis, using an antiserum raised against the invertebrate peptide, FMRFamide. Immunopositive neurones were found throughout the central nervous system in the ventral and dorsal nerve cords and the circumpharyngeal. and perianal nerve rings. Reactive neurones were also present peripherally, innervating the highly muscular pharynx with a nerve net and ring-like structures. Positive immunostaining was also observed in neurones running toward the stylet protractor muscles and/or the anterior sensory apparatus. This study implicates a role for FaRPs in feeding, host penetration and sensory function of PCN. This is the first study to characterise FaRP encoding genes from a plant-parasitic nematode using a targeted PCR based RACE approach and further underlines the importance and diversity of this neuropeptide group in the phylum Nematoda. (C) 2001 Elsevier Science B.V. All rights reserved.
LanguageEnglish
Pages199-208
JournalMolecular and Biochemical Parasitology
Volume116
Issue number2
Publication statusPublished - Sep 2001

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cyst nematodes
potatoes
peptides
nerve tissue
neurons
Globodera pallida
Globodera rostochiensis
genes
stylets
pharynx
neuropeptides
plant parasitic nematodes
central nervous system
antiserum
open reading frames
invertebrates
Nematoda
muscles

Cite this

Kimber, MJ., Fleming, CC., Bjourson, AJ., Halton, DW., & Maule, AG. (2001). FMRFamide-related peptides in potato cyst nematodes. Molecular and Biochemical Parasitology, 116(2), 199-208.
Kimber, MJ ; Fleming, CC ; Bjourson, AJ ; Halton, DW ; Maule, AG. / FMRFamide-related peptides in potato cyst nematodes. In: Molecular and Biochemical Parasitology. 2001 ; Vol. 116, No. 2. pp. 199-208.
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Kimber, MJ, Fleming, CC, Bjourson, AJ, Halton, DW & Maule, AG 2001, 'FMRFamide-related peptides in potato cyst nematodes', Molecular and Biochemical Parasitology, vol. 116, no. 2, pp. 199-208.

FMRFamide-related peptides in potato cyst nematodes. / Kimber, MJ; Fleming, CC; Bjourson, AJ; Halton, DW; Maule, AG.

In: Molecular and Biochemical Parasitology, Vol. 116, No. 2, 09.2001, p. 199-208.

Research output: Contribution to journalArticle

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AU - Kimber, MJ

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AU - Halton, DW

AU - Maule, AG

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N2 - This study presents data demonstrating the presence of FMRFamide-related peptides (FaRPs) in potato cyst nematodes (PCN). Five transcripts of FaRP encoding genes, designated gpflp-1 to gpflp-5, were characterised using RACE. In terms of ORFs, gpflp-1 was 444 base pairs (bp) long and coded for four copies of the FaRP, PF3 (KSAYMRFamide) whilst gpflp-2 was 309 bp long and encoded one copy of the peptide, KNKFEFIRFamide. gpflp-3 (420 bp) Encoded two copies of KHEYLRFamide (AF2) and the genes gpflp-4 and gpflp-5 encoded a total of 11 FaRPs, most of which are novel to PCN. FMRFamide-related peptide (FaRP)-like immunoreactivity was observed in both PCN species, Globodera pallida and Globodera rostochiensis, using an antiserum raised against the invertebrate peptide, FMRFamide. Immunopositive neurones were found throughout the central nervous system in the ventral and dorsal nerve cords and the circumpharyngeal. and perianal nerve rings. Reactive neurones were also present peripherally, innervating the highly muscular pharynx with a nerve net and ring-like structures. Positive immunostaining was also observed in neurones running toward the stylet protractor muscles and/or the anterior sensory apparatus. This study implicates a role for FaRPs in feeding, host penetration and sensory function of PCN. This is the first study to characterise FaRP encoding genes from a plant-parasitic nematode using a targeted PCR based RACE approach and further underlines the importance and diversity of this neuropeptide group in the phylum Nematoda. (C) 2001 Elsevier Science B.V. All rights reserved.

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M3 - Article

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EP - 208

JO - Molecular and Biochemical Parasitology

T2 - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

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Kimber MJ, Fleming CC, Bjourson AJ, Halton DW, Maule AG. FMRFamide-related peptides in potato cyst nematodes. Molecular and Biochemical Parasitology. 2001 Sep;116(2):199-208.