Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

Martin Trewhella; Richard Owusu; Arthur Finch

doi:10.1016/0167-4838(85)90284-5

Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

Martin Trewhella
, Richard Owusu
, Arthur Finch

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined

Original language	English
Pages (from-to)	282-287
Journal	BBA - Biochimica et Biophysica Acta
Volume	830
Issue number	3
DOIs	https://doi.org/10.1016/0167-4838(85)90284-5
Publication status	Published (in print/issue) - 25 Aug 1985

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10.1016/0167-4838(85)90284-5

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@article{1f94ba7216f24cea86c291e3a6cd229e,

title = "Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system",

abstract = "The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined",

author = "Martin Trewhella and Richard Owusu and Arthur Finch",

year = "1985",

month = aug,

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doi = "10.1016/0167-4838(85)90284-5",

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T1 - Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

AU - Trewhella, Martin

AU - Owusu, Richard

AU - Finch, Arthur

PY - 1985/8/25

Y1 - 1985/8/25

N2 - The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined

AB - The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined

U2 - 10.1016/0167-4838(85)90284-5

DO - 10.1016/0167-4838(85)90284-5

M3 - Article

VL - 830

SP - 282

EP - 287

JO - BBA - Biochimica et Biophysica Acta

JF - BBA - Biochimica et Biophysica Acta

IS - 3

ER -

Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

Abstract

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