TY - JOUR
T1 - Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system
AU - Trewhella, Martin
AU - Owusu, Richard
AU - Finch, Arthur
PY - 1985/8/25
Y1 - 1985/8/25
N2 - The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined
AB - The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined
U2 - 10.1016/0167-4838(85)90284-5
DO - 10.1016/0167-4838(85)90284-5
M3 - Article
VL - 830
SP - 282
EP - 287
JO - BBA - Biochimica et Biophysica Acta
JF - BBA - Biochimica et Biophysica Acta
IS - 3
ER -