Flow microcalorimetric study of immobilized enzyme kinetics using the urea-immobilized urease system

Martin Trewhella, Richard Owusu, Arthur Finch

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The use of flow microcalorimetry to study the kinetics of urea hydrolysis catalyzed by urease bound to porous glass (CPG) particles is described. The present approach involves measurement of the average, rather than initial, rate of reaction. The external and internal effectiveness factors and the intrinsic Michaelis constants for immobilized urease have been determined
Original languageEnglish
Pages (from-to)282-287
JournalBBA - Biochimica et Biophysica Acta
Volume830
Issue number3
DOIs
Publication statusPublished - 25 Aug 1985

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