Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: Simplex optimisation of media supplements

S Gough, O Flynn, Catherine Hack, Roger Marchant

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentrations of the supplements were 0.576 gl(-1) magnesium sulphate, 0.288 gl(-1) potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.
LanguageEnglish
Pages187-190
JournalApplied Microbiology and Biotechnology
Volume46
Issue number2
Publication statusPublished - Sep 1996

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Kluyveromyces marxianus
molasses
ethanol production
fermentation
yeasts
potassium dihydrogen phosphate
ethanol
sucrose
mead
magnesium sulfate
linseed oil
system optimization
nitrogen
ammonium sulfate
magnesium
potassium
fatty acids

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title = "Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: Simplex optimisation of media supplements",
abstract = "The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4{\%} (v/v) was produced from unsupplemented molasses at a concentration of 23{\%} (v/v). The effect on ethanol production of increasing the sucrose concentration in 23{\%} (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentrations of the supplements were 0.576 gl(-1) magnesium sulphate, 0.288 gl(-1) potassium dihydrogen phosphate and 0.36{\%} (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.",
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journal = "Applied Microbiology and Biotechnology",
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Fermentation of molasses using a thermotolerant yeast, Kluyveromyces marxianus IMB3: Simplex optimisation of media supplements. / Gough, S; Flynn, O; Hack, Catherine; Marchant, Roger.

In: Applied Microbiology and Biotechnology, Vol. 46, No. 2, 09.1996, p. 187-190.

Research output: Contribution to journalArticle

TY - JOUR

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N2 - The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentrations of the supplements were 0.576 gl(-1) magnesium sulphate, 0.288 gl(-1) potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.

AB - The use of molasses as a substrate for ethanol production by the thermotolerant yeast Kluyveromyces marxianus var. marxianus was investigated at 45 degrees C. A maximum ethanol concentration of 7.4% (v/v) was produced from unsupplemented molasses at a concentration of 23% (v/v). The effect on ethanol production of increasing the sucrose concentration in 23% (v/v) molasses was determined. Increased sucrose concentration had a similar detrimental effect on the final ethanol produced as the increase in molasses concentration. This indicated that the effect may be due to increased osmotic activity as opposed to other components in the molasses. The optimum concentration of the supplements nitrogen, magnesium, potassium and fatty acid for maximum ethanol production rate was determined using the Nelder and Mead (Computer J 7:308-313, 1965) simplex optimisation method. The optimum concentrations of the supplements were 0.576 gl(-1) magnesium sulphate, 0.288 gl(-1) potassium dihydrogen phosphate and 0.36% (v/v) linseed oil. Added nitrogen in the form of ammonium sulphate did not affect the ethanol production rate.

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